Evaluating adhesion and alignment of dental pulp stem cells to a spider silk substrate for tissue engineering applications

2017 ◽  
Vol 81 ◽  
pp. 104-112 ◽  
Author(s):  
Katherine Hafner ◽  
Dallas Montag ◽  
Hannah Maeser ◽  
Congyue Peng ◽  
William R. Marcotte ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Pulp ◽  
Spider Silk ◽  
Dental Pulp Stem Cells ◽  
Engineering Applications

scholarly journals METTL3-mediated M6a Modification Regulates Cell Cycle Progression of Dental Pulp Stem Cells

2021 ◽  
Author(s):  
Haiyun Luo ◽  
Wenjing Liu ◽  
Yanli Zhang ◽  
Xiao Jiang ◽  
Shiqing Wu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Flow Cytometry ◽  
Differentially Expressed Genes ◽  
Dental Pulp ◽  
Cell Cycle Control ◽  
Dental Pulp Stem Cells ◽  
Differentially Expressed ◽  
Expression Level

Abstract Background: Dental pulp stem cells (DPSCs) exhibited self-renewal, pluripotency capacity and served as promising cells source in endodontic regeneration and tissue engineering. Meanwhile, the regenerative capacity of DPSCs is limited and reduced in long lifespan. N6-methyladenosine (m6A) is the most prevalent, reversible internal modification in RNAs. The methyltransferases complex and demethylases mediated m6A methylation and cooperated to impact various biological processes associated with stem cell fate determination. However, the biological effect of m6A methylation in DPSCs remained unclear. Methods: Cell surface markers and differentiation potential of primary DPSCs were identified and m6A immunoprecipitation with deep sequencing (m6A RIP-seq) was used to uncover characteristics of m6A modifications in DPSCs transcriptome. Expression level of m6A-related genes were evaluated in immature/mature pulp tissues and cells. Lentiviral vectors were constructed to knockdown or overexpress methyltransferase like 3 (METTL3). Cell morphology, viability, senescence and apoptosis were further analyzed by β-galactosidase, TUNEL staining and flow cytometry. Bioinformatic analysis combing m6A RIP and shMETTL3 RNA-seq was used to functionally enrich overlapped genes and screen target of METTL3. Cell cycle distributions were assayed by flow cytometry and m6A RIP-qPCR was used to confirm METTL3 mediated m6A methylation in DPSCs. Results: Here, m6A peaks distribution, binding area and motif in DPSCs were first revealed by m6A RIP-seq. We also found a relative high expression level of METTL3 in immature DPSCs with superior regenerative potential and METTL3 knockdown induced cell apoptosis and senescence. Furthermore, Conjoint analysis of m6A RIP and RNA-sequencing showed differentially expressed genes affected by METTL3 depletion was mainly enriched in cell cycle, mitosis and alteration of METTL3 expression resulted in cell cycle arrest which indicated METTL3 make essential effect in cell cycle control. To further investigate underlying mechanisms, we explored proteins interaction network of differentially expressed genes and Polo-like Kinase 1 (PLK1), a critical cycle modulator was identified as target of METTL3-mediated m6A methylation in DPSCs. Conclusions: These results revealed m6A methylated hallmarks in DPSCs and a regulatory role of METTL3 in cell cycle control. Our study shed light on therapeutic approaches in vital pulp therapy and serve new insight in stem cells based tissue engineering.

scholarly journals Dental Pulp Stem Cells and Tissue Engineering Strategies for Clinical Application on Odontoiatric Field

10.5772/24871 ◽  
2011 ◽  
Author(s):  
Zavan Barbara ◽  
Bressan Eriberto ◽  
Sivolella Stefano ◽  
Brunello Giulia ◽  
Gardin Chiara ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Clinical Application ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells

scholarly journals Dental pulp stem cells express tendon markers under mechanical loading and are a potential cell source for tissue engineering of tendon-like tissue

2016 ◽  
Vol 8 (4) ◽  
pp. 213-222 ◽  
Author(s):  
Yu-Ying Chen ◽  
Sheng-Teng He ◽  
Fu-Hua Yan ◽  
Peng-Fei Zhou ◽  
Kai Luo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mechanical Loading ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Cell Source

scholarly journals Bladder Smooth Muscle Cells Differentiation from Dental Pulp Stem Cells: Future Potential for Bladder Tissue Engineering

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Bing Song ◽  
Wenkai Jiang ◽  
Amr Alraies ◽  
Qian Liu ◽  
Vijay Gudla ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Dental Pulp ◽  
Transforming Growth Factor ◽  
Dental Pulp Stem Cells ◽  
Specific Marker ◽  
Bladder Tissue ◽  
Growth Environment ◽  
Gene And Protein Expression

Dental pulp stem cells (DPSCs) are multipotent cells capable of differentiating into multiple cell lines, thus providing an alternative source of cell for tissue engineering. Smooth muscle cell (SMC) regeneration is a crucial step in tissue engineering of the urinary bladder. It is known that DPSCs have the potential to differentiate into a smooth muscle phenotype in vitro with differentiation agents. However, most of these studies are focused on the vascular SMCs. The optimal approaches to induce human DPSCs to differentiate into bladder SMCs are still under investigation. We demonstrate in this study the ability of human DPSCs to differentiate into bladder SMCs in a growth environment containing bladder SMCs-conditioned medium with the addition of the transforming growth factor beta 1 (TGF-β1). After 14 days of exposure to this medium, the gene and protein expression of SMC-specific marker (α-SMA, desmin, and calponin) increased over time. In particular, myosin was present in differentiated cells after 11 days of induction, which indicated that the cells differentiated into the mature SMCs. These data suggested that human DPSCs could be used as an alternative and less invasive source of stem cells for smooth muscle regeneration, a technology that has applications for bladder tissue engineering.

Tissue Engineering of Necrotic Dental Pulp of Immature Teeth with Apical Periodontitis in Dogs: Radiographic and Histological Evaluation

2018 ◽  
Vol 42 (5) ◽  
pp. 373-382 ◽  
Author(s):  
Eman A El Ashiry ◽  
Najlaa M Alamoudi ◽  
Mahmoud K El Ashiry ◽  
Hagar A Bastawy ◽  
Douaa A El Derwi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factors ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Apical Periodontitis ◽  
Permanent Teeth ◽  
Significant Difference ◽  
Group A ◽  
Group B

Aim: To evaluate tissue engineering technology to regenerate pulp-dentin like tissues in pulp canals of immature necrotic permanent teeth with apical periodontitis in dogs. Study design: The study was performed on 36 teeth in 12 dogs. The experiment was carried out using split mouth design. In each dog 3 teeth were selected for implementing the study procedure. Apical periodontitis was induced in Group A and B teeth. Group (A): immature upper left 2nd permanent incisors that were transplanted with a construct of autologous dental pulp stem cells with growth factors seeded in a chitosn hydrogel scaffold. Group (B): immature upper right 2nd permanent incisor that received only growth factors with scaffold. A third tooth in each dog was selected randomly for isolation of dental pulp stem cells (DPSCs). Both groups were closed with a double coronal seal of white MTA (Mineral trioxide aggregate) and glass ionomer cement. Both groups were monitored radiographically for 4 months and histologically after sacrificing the animals. Results: There was no statistically significant difference in radiographic findings between group (A) and group (B) for healing of radiolucencies, while there was statistically significant difference between group (A) and group (B) regarding radicular thickening, root lengthening and apical closure. Histologically, group (A) teeth showed regeneration of pulp- dentin like tissue while group (B) teeth did not show any tissue regeneration. Conclusion: Dental pulp stem cells and growth factors incorporated in chitosan hydrogel are able to regenerate pulp- dentine like tissue and help in complete root maturation of non-vital immature permanent teeth with apical periodontitis in dogs.

Dental pulp stem cells immobilized in alginate microspheres for applications in bone tissue engineering

2013 ◽  
Vol 47 (7) ◽  
pp. 687-697 ◽  
Author(s):  
M. M. Kanafi ◽  
A. Ramesh ◽  
P. K. Gupta ◽  
R. R. Bhonde
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells
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