The influence of adherent cell morphology on hydrodynamic recruitment of leukocytes

Tantalum is one of the most important biomaterials used for surgical implant devices. However, little knowledge exists about how nanoscale-textured tantalum surfaces affect cell morphology. Mammalian (Vero) cell morphology on tantalum-coated comb structures was studied using high-resolution scanning electron microscopy and fluorescence microscopy. These structures contained parallel lines and trenches with equal widths in the range of 0.18 to 100 μm. Results showed that as much as 77% of adherent cell nuclei oriented within 10° of the line axes when deposited on comb structures with widths smaller than 10 μm. However, less than 20% of cells exhibited the same alignment performance on blanket tantalum films or structures with line widths larger than 50 μm. Two types of line-width-dependent cell morphology were observed. When line widths were smaller than 0.5 μm, nanometer-scale pseudopodia bridged across trench gaps without contacting the bottom surfaces. In contrast, pseudopodia structures covered the entire trench sidewalls and the trench bottom surfaces of comb structures with line-widths larger than 0.5 μm. Furthermore, results showed that when a single cell simultaneously adhered to multiple surface structures, the portion of the cell contacting each surface reflected the type of morphology observed for cells individually contacting the surfaces.

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In Vitro Cell Adhesion and Detachment Behavior for Various Biomaterial Substrates

MRS Proceedings ◽

10.1557/proc-252-193 ◽

1991 ◽

Vol 252 ◽

Author(s):

Mary Beth Schmidt ◽

John B. Brunski

Keyword(s):

Cell Adhesion ◽

Cell Morphology ◽

Flow Chamber ◽

Adherent Cell ◽

Cell Detachment ◽

3T3 Fibroblasts ◽

Quantitative Examination ◽

Substrate Surfaces ◽

Cp Ti

ABSTRACTCell-biomaterial interactions for different material compositions were evaluated through quantitative examination of in vitro cell spreading behavior and shear-induced cell detachment. Biomaterial substrates consisted of identically prepared, well characterized bulk samples of implant grade cp Ti, Ti-6AI-4V, and Synamel. 3T3 fibroblasts were seeded on the substrate surfaces and incubated for 2 hours. Adherent cell morphology was then examined using scanning electron microscopy and digital image analysis. The cell detachment response was measured using a parallel plate flow chamber. When categorized according to observed cell morphology, projected cell areas were found to be lognormally distributed for all biomaterials and ln(cell area) was significantly greater for Synamel, while no difference existed between cp Ti and Ti-6AI-4V. In addition, the average post-shear fraction of adherent cells was significantly greater for Synamel substrates, as compared to cp Ti and Ti-6AI-4V substrates. The results from this study indicate that in vitro cell adhesion and detachment behavior are measurably influenced by biomaterial substrate composition.

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Author response for "Differential temporal and spatial post‐injury alterations in cerebral cell morphology and viability"

10.1002/cne.24955/v3/response1 ◽

2020 ◽

Author(s):

Zareen Amtul ◽

Abdullah N. Najdat ◽

David J. Hill ◽

Edith J. Arany

Keyword(s):

Cell Morphology ◽

Author Response ◽

Post Injury ◽

Temporal And Spatial

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Cell morphology, volume, and surface area determinations from multilevel contouring within HVEM micrographs of serial sections and whole-cell mounts

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100127426 ◽

1987 ◽

Vol 45 ◽

pp. 588-589

Author(s):

M. Marko ◽

A. Leith ◽

D. Parsons

Keyword(s):

Surface Area ◽

Electron Micrographs ◽

Cell Morphology ◽

Individual Variation ◽

Serial Section ◽

Stereo Pair ◽

Complex Structures ◽

Serial Sections ◽

Surface Areas ◽

Computer Based

The use of serial sections and computer-based 3-D reconstruction techniques affords an opportunity not only to visualize the shape and distribution of the structures being studied, but also to determine their volumes and surface areas. Up until now, this has been done using serial ultrathin sections.The serial-section approach differs from the stereo logical methods of Weibel in that it is based on the Information from a set of single, complete cells (or organelles) rather than on a random 2-dimensional sampling of a population of cells. Because of this, it can more easily provide absolute values of volume and surface area, especially for highly-complex structures. It also allows study of individual variation among the cells, and study of structures which occur only infrequently.We have developed a system for 3-D reconstruction of objects from stereo-pair electron micrographs of thick specimens.

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