Intra-isolate heterogeneity of the ITS region of rDNA in Pythium helicoides

2007 ◽  
Vol 111 (4) ◽  
pp. 416-423 ◽  
Author(s):  
Koji Kageyama ◽  
Masako Senda ◽  
Takahiro Asano ◽  
Haruhisa Suga ◽  
Kiyoshi Ishiguro
Keyword(s):  
Its Region ◽  
Pythium Helicoides

Localization of cell-surface and basement-membrane heparan-sulfate proteoglycans using the malaria circumsporozoite protein

1994 ◽  
Vol 52 ◽  
pp. 294-295
Author(s):  
U. Frevert ◽  
S. Sinnis ◽  
C. Cerami ◽  
V. Nussenzweig
Keyword(s):  
Heparan Sulfate ◽  
Protein A ◽  
Kidney Tissue ◽  
Plasmodium Species ◽  
Its Region ◽  
Basement Membranes ◽  
Heparan Sulfate Proteoglycans ◽  
Infected Mosquito ◽  
Complement Components ◽  
Region Ii

Malaria sporozoites, which invade hepatocytes within minutes after transmission by an infected mosquito, are covered with the circumsporozoite (CS) protein, which in all Plasmodium species contains the conserved region II-plus. This region is also found as a cell-adhesive motif in a variety of host proteins like thrombospondin, properdin and the terminal complement components.The CS protein with its region II-plus specifically binds to heparan sulfate proteoglycans (HSPG) on the basolateral surface of hepatocytes in the space of Disse (FIG. 1), to certain basolateral cell membranes and basement membranes of the kidney (FIG. 2) as well as to heparin in the granules of connective tissue mast cells. The distribution of the HSPG receptors for the CS protein was examined by incubation of Lowicryl K4M or LR White sections of liver and kidney tissue with the recombinant CS ligand, whose binding sites were detected with a monoclonal anti-CS antibody and protein A gold.

Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region

1999 ◽  
Vol 37 (2) ◽  
pp. 105-114 ◽  
Author(s):  
Y. GRAser ◽  
M. EL Fari ◽  
R. Vilgalys ◽  
A. F. A. Kuijpers ◽  
G. S. DE Hoog ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Its Region ◽  
The Family

409 The likely numbers to come: trends in hospitalisation for heart failure in the ‘sample’ town of trieste and its region

2003 ◽  
Vol 2 (1) ◽  
pp. 84
Author(s):  
D CHERSEVANI ◽  
A DILENARDA ◽  
P GOLIANI ◽  
M GRELLA ◽  
F BRUN ◽  
...  
Keyword(s):  
Heart Failure ◽  
Its Region ◽  
To Come

Molecular detection of Murshidia linstowi in a free-ranging dead elephant calf

2020 ◽  
Vol 12 (3) ◽  
pp. 15359-15363
Author(s):  
Sourabh Ranjan Hota ◽  
Sonali Sahoo ◽  
Manojita Dash ◽  
Avishek Pahari ◽  
Bijayendranath Mohanty ◽  
...  
Keyword(s):  
Average Length ◽  
Its Region ◽  
Buccal Capsule ◽  
Nematode Species ◽  
Phylogenetic Study ◽  
Gastrointestinal Helminths ◽  
Pcr Product ◽  
Maximum Similarity ◽  
Host Parasite ◽  
Free Ranging

Gastrointestinal helminths are ubiquitous in both domestic and wild animals. Infections are often sub-clinical except in circumstances of destabilization of host-parasite equilibrium by innate or environmental factors. The present case deals with microscopic and molecular diagnosis of Murshidia linstowi recovered from an elephant. A post-mortem examination of a free-ranging juvenile male elephant calf that had died of electrocution in Athagarh Wildlife Division revealed the presence of slender, whitish nematodes in the stomach. No gross lesions were noticed either in the site of predilection or any other internal organs. The average length of the parasites was 3.8cm.  These parasites were collected for further gross as well as microscopic examination following routine parasitological techniques. Temporary mounts prepared after cleaning the nematodes in lactophenol were observed under a microscope. Morphological features such as a well-developed mouth collar, large and globular buccal capsule with fine tubercles, cone shaped oesophageal funnel, short bursa having indistinctly divided lobes and closely apposed ventral rays and stout spicules with club shaped tips bent dorsally corroborated with that of M.linstowi (male). Amplification of the rDNA from the internal transcribed spacer (ITS) region using universal nematode primers NC2 and NC5 revealed a product size of 870bp. The PCR product was subjected to sequencing followed by NCBI-BLAST which revealed 98% homology with M. linstowi. A phylogenetic study showed a maximum similarity with M.linstowi recovered from elephants in Kenya. This particular nematode species belonging to the family Strongylidae and sub-family Cyathostominae appears to be the first documented report in India.

Chestnuts in Charters

2018 ◽  
Author(s):  
Ross Balzaretti
Keyword(s):  
Comparative Method ◽  
Its Region ◽  
Medieval Period ◽  
Social Differences ◽  
Early Medieval Period ◽  
Specialized Production ◽  
System P ◽  
Macro Scale ◽  
Two Cities ◽  
Economic Specialization

This chapter responds to a point which Chris Wickham raised in his recent review of my book on Dark Age Liguria: did chestnut cultivation show any economic specialization in this region in the early medieval period? Chestnuts figured a great deal in that book, which drew briefly on the surviving charter documentation for the region. In this chapter a more detailed analysis of charters from the tenth and eleventh centuries develops an answer to the question of specialized production with a comparative study in which the Genoese evidence is set alongside similar charter evidence from Milan and its region, where chestnuts were also cultivated for food. The Genoa–Milan comparison puts into practice Wickham’s advocacy of comparative method at the micro as well as at the macro scale, for regions where comparison has not historically been the norm. The comparison suggests that chestnuts were more important to the Genoese than the Milanese economy, in part for local climatic reasons but also, perhaps, because of fundamental political and social differences between these two cities. It will be shown that some charters show that the production of chestnuts was to some degree specialized, how it was specialized and what the consequences of that specialization were for each economic system.

scholarly journals The Patterns and Puzzles of Genetic Diversity of Endangered Freshwater Mussel Unio crassus Philipsson, 1788 Populations from Vistula and Neman Drainages (Eastern Central Europe)

Life ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 119
Author(s):  
Adrianna Kilikowska ◽  
Monika Mioduchowska ◽  
Anna Wysocka ◽  
Agnieszka Kaczmarczyk-Ziemba ◽  
Joanna Rychlińska ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Spatial Scales ◽  
Freshwater Mussel ◽  
Its Region ◽  
Strong Relationship ◽  
Mantel Test ◽  
Freshwater Ecosystems ◽  
Hierarchical Organization ◽  
Anthropogenic Factors

Mussels of the family Unionidae are important components of freshwater ecosystems. Alarmingly, the International Union for Conservation of Nature and Natural Resources Red List of Threatened Species identifies almost 200 unionid species as extinct, endangered, or threatened. Their decline is the result of human impact on freshwater habitats, and the decrease of host fish populations. The Thick Shelled River Mussel Unio crassus Philipsson, 1788 is one of the examples that has been reported to show a dramatic decline of populations. Hierarchical organization of riverine systems is supposed to reflect the genetic structure of populations inhabiting them. The main goal of this study was an assessment of the U. crassus genetic diversity in river ecosystems using hierarchical analysis. Different molecular markers, the nuclear ribosomal internal transcribed spacer ITS region, and mitochondrial DNA genes (cox1 and ndh1), were used to examine the distribution of U. crassus among-population genetic variation at multiple spatial scales (within rivers, among rivers within drainages, and between drainages of the Neman and Vistula rivers). We found high genetic structure between both drainages suggesting that in the case of the analyzed U. crassus populations we were dealing with at least two different genetic units. Only about 4% of the mtDNA variation was due to differences among populations within drainages. However, comparison of population differentiation within drainages for mtDNA also showed some genetic structure among populations within the Vistula drainage. Only one haplotype was shared among all Polish populations whereas the remainder were unique for each population despite the hydrological connection. Interestingly, some haplotypes were present in both drainages. In the case of U. crassus populations under study, the Mantel test revealed a relatively strong relationship between genetic and geographical distances. However, in detail, the pattern of genetic diversity seems to be much more complicated. Therefore, we suggest that the observed pattern of U. crassus genetic diversity distribution is shaped by both historical and current factors i.e. different routes of post glacial colonization and history of drainage systems, historical gene flow, and more recent habitat fragmentation due to anthropogenic factors.

scholarly journals Cryptococcus rajasthanensis sp. nov., an anamorphic yeast species related to Cryptococcus laurentii, isolated from Rajasthan, India

2007 ◽  
Vol 57 (2) ◽  
pp. 414-418 ◽  
Author(s):  
Puja Saluja ◽  
G. S. Prasad
Keyword(s):  
Large Subunit ◽  
Its Region ◽  
Molecular Data ◽  
Lsu Rdna ◽  
Cryptococcus Laurentii ◽  
The Novel ◽  
D2 Domain ◽  
Its Regions ◽  
Anamorphic Yeast ◽  
Physiological Tests

Two novel anamorphic yeast strains (S-15LT and 3-C1) were isolated from the inflorescences of plants collected in two different towns in Rajasthan State, India. Sequencing of the D1/D2 domains of the large-subunit (LSU) rDNA and the internal transcribed spacer (ITS) regions suggested they are strains of the same species. Phenotypic characteristics such as the absence of fermentation, the absence of sexual structures and ballistoconidia, the assimilation of myo-inositol and d-glucuronate, and positive Diazonium blue B and urease reactions indicated that these strains belong to the genus Cryptococcus. The novel strains differed from Cryptococcus laurentii in six physiological tests and differed from other related species in more than six tests. A phylogenetic analysis of the sequences of the D1/D2 domains of the LSU rDNA and the ITS regions placed these strains in the Bulleromyces clade within the order Tremellales, with C. laurentii as their closest described relative. The novel strains showed 1.6 and 7.5 % divergence in the D1/D2 domain of the LSU rDNA and ITS regions, respectively, with respect to C. laurentii. The divergence from other species was more than 3 % for the D1/D2 domain and more than 9 % for the ITS region. On the basis of the phenotypic and molecular data, strains S-15LT and 3-C1 represent a novel species within the genus Cryptococcus, for which the name Cryptococcus rajasthanensis sp. nov. is proposed. The type strain is S-15LT (=MTCC 7075T=CBS 10406T).

scholarly journals Biodiversity of non-Saccharomyces yeasts associated with spontaneous fermentation of Cabernet Sauvignon wines from Shangri-La wine region, China

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yue Zhao ◽  
Qingyang Sun ◽  
Shusheng Zhu ◽  
Fei Du ◽  
Ruzhi Mao ◽  
...  
Keyword(s):  
Yeast Species ◽  
Its Region ◽  
Cabernet Sauvignon ◽  
Jinsha River ◽  
Spontaneous Fermentation ◽  
Lancang River ◽  
5.8S Rrna ◽  
Colonial Morphology ◽  
26S Rrna ◽  
Wine Region

AbstractShangri-La is a wine region that has the highest altitude vineyards in China. This is the first study investigated the biodiversity of non-Saccharomyces yeasts associated with spontaneous fermentation of Cabernet Sauvignon wines produced from two sub-regions (Lancang River and Jinsha River) of Shangri-La. The culturable yeasts were preliminarily classified based on their colonial morphology on the Wallerstein Laboratory nutrient agar plates. Yeast species were identified by the sequencing of the 26S rRNA D1/D2 region and the 5.8S rRNA ITS region. Twenty-five non-Saccharomyces yeast species belonging to sixteen genera were isolated and identified in Shangri-La wine region. Candida, Hanseniaspora, Pichia, and Starmerella were found in both sub-regions, but the Lancang River showed more diverse yeast species than the Jinsha River. Shangri-La not only exhibited high diversity of non-Saccharomyces yeasts, and furthermore, seven species of non-Saccharomyces yeasts were exclusively found in this region, including B. bruxellensis, D. hansenii, M. guilliermondii, S. vini, S. diversa, T. delbrueckii and W. anomalus, which might play an important role in distinctive regional wine characteristics. This study provide a relatively comprehensive analysis of indigenous non-Saccharomyces yeasts associated with Cabernet Sauvignon from Shangri-La, and has significance for exploring ‘microbial terroir’ of wine regions in China.

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