Research on Fundus Image Mosaic Method Based on Genetic Algorithm

Retinal image mosaic is the key to detect common diseases, and the existing image mosaic methods are difficult to solve the problems of low contrast of fundus images and geometric distortion between images in different fields of view. To solve the problem of noise in retinal fundus images, an image mosaic algorithm based on the genetic algorithm was proposed. Firstly, a series of morphological pretreatment was performed on the fundus images. Then, the vascular network is extracted by obtaining the maximum entropy of the image to determine the threshold value. The similarity of the image to be spliced is a feature, and the genetic algorithm is used to solve the optimal parameters to achieve the maximum similarity. By smoothing the image, a clear image with minimum noise is obtained. Experimental results show that the proposed algorithm can effectively realize the image mosaic of the fundus. The method proposed in this paper can provide support for high-precision automatic stitching of multiple single-mode color fundus images.

SSR based molecular profiling of elite cultivars of basmati rice (Oryza sativa L.)

The experiment was conducted to assess the available genetic variability amongst Indian Basmati rice and identify co-dominant and reproducible robust simple sequence repeat markers for drought resistance and their utilization in marker assisted selection for developing drought resistant / tolerant aromatic rice cultivars in various parts of India as well as in world. DNA was isolated from fresh and young leaf tissues of 35 cultivars of basmati rice using the CTAB procedure of Doyle and Doyle with slightly modifications. The DNA was further quantified by spectrophotometer at 260 nm and 280 nm. The quality and quantity of DNA were checked by agarose gel electrophoresis. Out of 60 SSR markers, 18 were found to be polymorphic and the rest 42 primers were monomorphic. One of the main purposes of SSR markers in genomic study is the characterization of genetic resources to help gene bank management. The informative (18) SSR markers were capable to discriminate the entire cultivars of basmati rice used in this research work. A total of 52 alleles at 18 loci could be scored. The allelic richness per locus diversified from 2 to 5 with an average of 2.89 alleles per locus. The amplitude of Polymorphism Information Content (PIC) value is 0.066 (RM 1068) to 0.730 (RM 1059) with an average of 0.505. The Jaccard’s similarity coefficient ranged from 0.79 to 0.95. Polymorphic finding content showed a positive correlation (r =0.69) with number of alleles at the SSR locus. However it is recommended that SSR markers can be efficiently utilized for this purpose. The maximum similarity coefficient was observed between Jeerakasab and Kalanamak with a coefficient value of 95% and the minimum similarity was found between Jeerakasab and Pusa basmati-1 with a coefficient value of 79%.

Brevibacillus composti sp. nov., isolated from hyperthermophilic compost

Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China. Growth was observed at 30–60 °C (optimum, 50 °C) and pH 6.0–9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%). Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus . The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The main polar lipids included phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%). Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus , for which the name Brevibacillus composti sp. nov. is proposed. The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).

Decadal comparison (1950–2020) of benthic marine flora from Central and Southern Vietnam

The benthic flora of Central and Southern Vietnam and its historical changes between 1950 and 2020 were studied. Three collections were compared: collection A (1950–1970), collection B (1980–2000) and collection С (2000–2020). In all collections (1950–2020), a total of 702 macroalgal taxa and their forms have been found, including 386 red (55%), 142 brown (20%) and 174 green (25%) algal taxa. A comparison of the collections has shown significant changes in species compositions of the marine flora during the period (the maximum similarity of the benthic flora of the three compared collections was about 63%). In each taxonomic group of macrophytes, there was a proportional replacement of some species by others. It is suggested that the revealed decadal changes in the marine flora of Central and Southern Vietnam are natural, periodic (seasonal, annual or perennial) and regulated by internal factors of the ecosystem.

Creative Opportunities of the Language in Online Communication

The paper reports the findings of the study of the implementation of the creative function of language in Internet communication. This research perspective allows to consistently describe the functioning of language online and to explain the visible and controversially viewed by academics (as innovative or as destructive) processes in the language system, occurring under the influence of the Internet environment. The revision of traditional standards of communication when communicating via Internet occurs, first of all, due to extralinguistic factors (primarily, digital transformation) and, secondly, due to the Internet interlocutors’ (both the speaker and the listener) attitude towards creative use of the language. The new extralinguistic conditions of language (multimedia, non-linear nature, its modules, hypertextuality, etc.) primarily release its creative potential as the novelty is inherently creative and requires the updating of traditional, familiar, and already studied forms of communication. The influence of the creative function of the language can be traced both at the micro levels of the language system (especially at the phonetic and lexical levels) and at the macro level of the whole text (the emergence of new genres). At the phonetic level, influenced by the creative function, the language of the Internet acquires a special spoken-written character. Moreover, the imitation of orality in Internet communication is aimed not only at achieving maximum similarity of online communication to offline conversation, but also at the language play, emotionalism, updating the graphic code with emoji (for example, the use of emoji as ending punctuation marks, i.e., instead of period, exclamation mark, etc.). At the lexical level, the creative function of the language is evident in the emergence of proper Russian neologisms and borrowings for more accurate, sometimes more succinct denotation of the new reality. At the textual level, the study, e.g., of the model Internet genre of multimedia history, the identification of its special narrative structure and the multimedia nature of presenting the information suggests the creative potential of new genres in the online communication.

Spatial-temporal distribution of birds in the northern macroslope of the Kyrgyz mountain ridge (Tien Shan)

Based on the results of our analysis, a classification of bird species of the Kyrgyz mountain ridge (Northern Tien Shan) was compiled according to their preference for habitats and occurrence throughout the year. The program of factorial classification used for this unites species according to the maximum similarity in their distribution and stay in an unspecified number of clusters. In total, 4 supertypes and 12 types of preferences have been identified along the Kyrgyz ridge. In the supertype of birds that prefer undeveloped areas, 6 types were distinguished, namely, the species choosing: 1 – high mountains, 2 – high and medium mountains, 3 – medium mountains, 4 – medium mountains and foothills, 5 – foothills, and 6 – found at all heights. The supertype of birds preferring built-up areas is represented by one type only: the species preferring foothill sheaths and villages, and the supertype of birds preferring rivers and their banks is represented by four types, namely, the species preferring: high-mountain rivers in the summer and autumn; high-altitude and mid-mountain rivers in the spring and summer; mid-mountain rivers in the autumn; and foothill rivers in the first half of the summer. The supertype uniting species found in all habitats is represented by one type of preference, namely, found throughout the whole year. It is shown that of the 154 recorded bird species, most were found in undeveloped habitats, and 6 and 12 times less were in built-up areas and rivers, including their banks. In undeveloped areas, most bird species prefer high- and mid-mountainous landscapes and half as many prefer foothill landscapes. In residential landscapes, most species tend to the foothills, and among rivers, they prefer high-altitude and mid-mountain areas. At to the seasons, the maximum number of species was recorded in the spring-summer and summer periods.

Complete genome sequence of Chryseobacterium sp. ZHDP1 with protease activity

This study reported a complete genome of Chryseobacterium sp. ZHDP1 isolated from the soils of a seafood market. The ZHDP1 genome with a size of 4,917,748 bp and a GC content of 35.95% possessed 4,478 coding genes, 5 rRNA genes, 26 sRNA genes, and 89 tRNA genes. The 16S rRNA gene sequence of ZHDP1 had a maximum similarity of 99.07% with that of C. gambrini 5-1St1a. The maximum values of average nucleotide identity and DNA-DNA hybridization of ZHDP1 genome were 91.39 and 47.8, respectively, which were lower than the thresholds for a new genome. Different protease genes were annotated in the genome of ZHDP1, and the protease activity was also detected in the fermentation broth of ZHDP1. Furthermore, the activity of protease in the fermentation broth was optimized through temperature, pH, and metal irons, and the results showed that 60°C and pH 7.0 were the optimum conditions and Fe3+ could positively increase the protease activity of ZHDP1. This study provides the first insight into the novel genomic information of Chryseobacterium sp. ZHDP1 and its protein-degrading ability, thereby broadening our knowledge of the industrial potentials in genus Chryseobacterium strains.

Automated Brain Tumor Prediction System using Natural Language Processing (NLP)

In this paper, we proposed an Automated Brain Tumor Prediction System which predicts Brain Tumor through symptoms in several diseases using Natural Language Processing (NLP). Term Frequency Inverse Document Frequency (TF-IDF) is used for calculating term weighting of terms on different disease’s symptoms. Cosine Similarity Measure and Euclidean Distance are used for calculating angular and linear distance respectively between diseases and symptoms for getting ranking of the Brain Tumor in the ranked diseases. A novel mathematical strategy is used here for predicting chance of Brain Tumor through symptoms in several diseases. According to the proposed novel mathematical strategy, the chance of the Brain Tumor is proportional to the obtained similarity value of the Brain Tumor when symptoms are queried and inversely proportional to the rank of the Brain Tumor in several diseases and the maximum similarity value of the Brain Tumor, where all symptoms of Brain Tumor are present.

Systemic mycobacteriosis along with concurrent fibroma in Goldfish, Carassius auratus from India

Aim: Goldfish (Carassius auratus L.), a freshwater fish belonging to the family Cyprinidae, is an important ornamental fish species in the world. Herewith, we report a case of systemic mycobacteriosis along with concurrent fibroma in goldfish. Methodology: The protruding mass and internal organs exhibiting lesions were collected for histopathology and molecular diagnosis. The sections of cutaneous mass were stained with haematoxylin and eosin (H&E) and Masson trichrome, whereas sections from spleen as well as kidney were stained with H&E and Ziehl-Neelsen. For identifying the etiological agent, 16S rRNA gene fragment was amplified and sequenced, using genus-specific primers for Mycobacterium sp. Results: In histopathology, multiple granulomas could be observed in kidney and spleen sections. Importantly, typical acid-fast bacilli were demonstrated in granulomas in spleen. The sequence of 16S rRNA gene fragment amplified from spleen and kidney showed maximum similarity to Mycobacterium stomatepiae. Besides, the histopathological characteristics of the protruding mass were consistent with cutaneous fibroma. Interpretation: These findings assume significance as several Mycobacterium species, including the current one from ornamental fish are reported to be of zoonotic significance.