Development of a Fluorescence In Situ Hybridization protocol for analysis of Gram-positive and Gram-negative bacteria

2016 ◽  
Vol 33 (3) ◽  
pp. 434
Author(s):  
Marina González-Pérez ◽  
Rafaela Fernandes ◽  
Ricardo Vieira ◽  
António Pereira ◽  
António Candeias ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Hybridization Protocol

scholarly journals Catalyzed Reported Deposition-Fluorescence In Situ Hybridization Protocol To Evaluate Phagotrophy in Mixotrophic Protists

2005 ◽  
Vol 71 (11) ◽  
pp. 7321-7326 ◽  
Author(s):  
Juan M. Medina-Sánchez ◽  
Marisol Felip ◽  
Emilio O. Casamayor
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Signal Amplification ◽  
Cell Attachment ◽  
Cell Loss ◽  
Alexa Fluor ◽  
Hybridization Protocol ◽  
Card Fish ◽  
First Time

ABSTRACT We describe a catalyzed reported deposition-fluorescence in situ hybridization (CARD-FISH) protocol particularly suited to assess the phagotrophy of mixotrophic protists on prokaryotes, since it maintains cell and plastid integrity, avoids cell loss and egestion of prey, and allows visualization of labeled prey against plastid autofluorescence. This protocol, which includes steps such as Lugol's-formaldehyde-thiosulfate fixation, agarose cell attachment, cell wall permeabilization with lysozyme plus achromopeptidase, and signal amplification with Alexa-Fluor 488, allowed us to detect almost 100% of planktonic prokaryotes (Bacteria and Archaea) and, for the first time, to show archaeal cells ingested by mixotrophic protists.

Higher axial-resolution and sensitivity pachytene fluorescence in situ hybridization protocol in tetraploid cotton

2009 ◽  
Vol 17 (8) ◽  
pp. 1041-1050 ◽  
Author(s):  
Kai Wang ◽  
Zaijie Yang ◽  
Changshen Shu ◽  
Jing Hu ◽  
Qiuyun Lin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tetraploid Cotton ◽  
Axial Resolution ◽  
Hybridization Protocol

scholarly journals Asymmetric one-pot synthesis of cyclopropanes

2016 ◽  
Vol 35 (1) ◽  
pp. 45
Author(s):  
Naoufel Ben Hamadi ◽  
Ahlem Guesmi ◽  
Wided Nouira
Keyword(s):  
Escherichia Coli ◽  
Water Soluble ◽  
Gram Negative Bacteria ◽  
Protecting Group ◽  
Gram Positive ◽  
One Pot ◽  
Gram Negative ◽  
Activity Screening ◽  
High Yields

Cycloaddition of the diazoalkanes to electron-deficient olefins (in situ) affords polysubstituted cyclopropanes in high yields (up to 85%). Deprotection of the ketal protecting group provided water-soluble cyclopropane-bearing carbohydrate in good yields. Antimicrobial activity screening of the synthesized compounds 8 and 9, utilizing a variety of Gram-positive (Staphylococcus aureus and Enterococcus fecalis), Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae) and yeast (Candida albicans), exhibited that all the prepared analogues acquire promising activities against both Gram-positive and Gram-negative bacteria especially compounds 9b and 9c (antimicrobial active agents against Gram-negative bacteria).

scholarly journals A modified fluorescence in situ hybridization protocol for Plasmodium falciparum greatly improves nuclear architecture conservation

2010 ◽  
Vol 173 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Monica Contreras-Dominguez ◽  
Carolina Borsoi Moraes ◽  
Thierry Dorval ◽  
Auguste Genovesio ◽  
Fernando de Macedo Dossin ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Nuclear Architecture ◽  
Hybridization Protocol

The in situ synthesis of silver nanoclusters inside a bacterial cellulose hydrogel for antibacterial applications

2020 ◽  
Vol 8 (22) ◽  
pp. 4846-4850 ◽  
Author(s):  
Yong Liu ◽  
Shanshan Wang ◽  
Ziping Wang ◽  
Qiaofeng Yao ◽  
Shasha Fang ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
In Situ Synthesis ◽  
Silver Nanoclusters ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Cellulose Hydrogel ◽  
Ag Nanoclusters ◽  
Ag Ncs

An antibacterial hydrogel is developed through in situ formation of Ag nanoclusters on bacterial cellulous, which shows better and longer-lasting killing efficacy than that of pristine Ag NCs against both Gram-positive and Gram-negative bacteria.

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