Repeated peripheral administration of lipidized prolactin-releasing peptide analog induces c-fos and FosB expression in neurons of dorsomedial hypothalamic nucleus in male C57 mice

2018 ◽  
Vol 116 ◽  
pp. 77-84 ◽  
Author(s):  
Zdenko Pirník ◽  
Mária Kolesárová ◽  
Blanka Železná ◽  
Lenka Maletínská
Keyword(s):  
Hypothalamic Nucleus ◽  
Peptide Analog ◽  
Dorsomedial Hypothalamic Nucleus ◽  
Prolactin Releasing Peptide

scholarly journals Assessing the treatment of cannabidiolic acid methyl ester: a stable synthetic analogue of cannabidiolic acid on c-Fos and NeuN expression in the hypothalamus of rats

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Eric Murillo-Rodríguez ◽  
Diana Millán-Aldaco ◽  
Gloria Arankowsky-Sandoval ◽  
Tetsuya Yamamoto ◽  
Roger G. Pertwee ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Cannabis Sativa ◽  
Brain Area ◽  
Acid Methyl Ester ◽  
Hypothalamic Nucleus ◽  
Synthetic Analogue ◽  
Neuronal Nuclei ◽  
Cannabidiolic Acid ◽  
Dorsomedial Hypothalamic Nucleus ◽  
Psychotropic Compound

Abstract Background Cannabidiol (CBD), the non-psychotropic compound from Cannabis sativa, shows positive results on controlling several health disturbances; however, comparable data regarding additional chemical from C. sativa, such as cannabidiolic acid (CBDA), is scarce due to its instability. To address this limitation, a stable CBDA analogue, CBDA methyl ester (HU-580), was synthetized and showed CBDA-like effects. Recently, we described that HU-580 increased wakefulness and wake-related neurochemicals. Objective To extend the comprehension of HU-580´s properties on waking, the c-Fos and NeuN expression in a wake-linked brain area, the hypothalamus was evaluated. Methods c-Fos and NeuN expression in hypothalamic sections were analyzed after the injections of HU-580 (0.1 or 100 μg/kg, i.p.). Results Systemic administrations of HU-580 increased c-Fos and neuronal nuclei (NeuN) expression in hypothalamic nuclei, including the dorsomedial hypothalamic nucleus dorsal part, dorsomedial hypothalamic nucleus compact part, and dorsomedial hypothalamic nucleus ventral part. Conclusion HU-580 increased c-Fos and NeuN immunoreactivity in hypothalamus nuclei suggesting that this drug might modulate the sleep–wake cycle by engaging the hypothalamus.

scholarly journals The Dorsomedial Hypothalamic Nucleus Is Not Necessary for the Expression of Circadian Food-Anticipatory Activity in Rats

2007 ◽  
Vol 22 (6) ◽  
pp. 467-478 ◽  
Author(s):  
Glenn J. Landry ◽  
Glenn R. Yamakawa ◽  
Ian C. Webb ◽  
Rhiannon J. Mear ◽  
Ralph E. Mistlberger
Keyword(s):  
Hypothalamic Nucleus ◽  
Food Anticipatory Activity ◽  
Dorsomedial Hypothalamic Nucleus ◽  
Anticipatory Activity

scholarly journals The deletion of glucagon-like peptide-1 receptors expressing neurons in the dorsomedial hypothalamic nucleus disrupts the diurnal feeding pattern and induces hyperphagia and obesity

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Yuko Maejima ◽  
Shoko Yokota ◽  
Masaru Shimizu ◽  
Shoichiro Horita ◽  
Daisuke Kobayashi ◽  
...  
Keyword(s):  
Nucleus Tractus Solitarius ◽  
Physiological Role ◽  
Feeding Pattern ◽  
Hypothalamic Nucleus ◽  
Acid Decarboxylase ◽  
Mrna Levels ◽  
Dorsomedial Hypothalamic Nucleus ◽  
Fos Expression ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Abstract Background Feeding rhythm disruption contributes to the development of obesity. The receptors of glucagon-like peptide-1 (GLP-1) are distributed in the wide regions of the brain. Among these regions, GLP-1 receptors (GLP-1R) are expressed in the dorsomedial hypothalamic nucleus (DMH) which are known to be associated with thermogenesis and circadian rhythm development. However, the physiological roles of GLP-1R expressing neurons in the DMH remain elusive. Methods To examine the physiological role of GLP-1R expressing neurons in the DMH, saporin-conjugated exenatide4 was injected into rat brain DMH to delete GLP-1R-positive neurons. Subsequently, locomotor activity, diurnal feeding pattern, amount of food intake and body weight were measured. Results This deletion of GLP-1R-positive neurons in the DMH induced hyperphagia, the disruption of diurnal feeding pattern, and obesity. The deletion of GLP-1R expressing neurons also reduced glutamic acid decarboxylase 67 and cholecystokinin A receptor mRNA levels in the DMH. Also, it reduced the c-fos expression after refeeding in the suprachiasmatic nucleus (SCN). Thirty percent of DMH neurons projecting to the SCN expressed GLP-1R. Functionally, refeeding after fasting induced c-fos expression in the SCN projecting neurons in the DMH. As for the projection to the DMH, neurons in the nucleus tractus solitarius (NTS) were found to be projecting to the DMH, with 33% of those neurons being GLP-1-positive. Refeeding induced c-fos expression in the DMH projecting neurons in the NTS. Conclusion These findings suggest that GLP-1R expressing neurons in the DMH may mediate feeding termination. In addition, this meal signal may be transmitted to SCN neurons and change the neural activities.

Metabolomic Study of Obesity and Its Treatment with Palmitoylated Prolactin-Releasing Peptide Analog in Spontaneously Hypertensive and Normotensive Rats

2019 ◽  
Vol 18 (4) ◽  
pp. 1735-1750 ◽  
Author(s):  
Martina Čermáková ◽  
Helena Pelantová ◽  
Barbora Neprašová ◽  
Blanka Šedivá ◽  
Lenka Maletínská ◽  
...  
Keyword(s):  
Spontaneously Hypertensive ◽  
Peptide Analog ◽  
Prolactin Releasing Peptide ◽  
Metabolomic Study

Organisation of the human dorsomedial hypothalamic nucleus

Neuroreport ◽  
2004 ◽  
Vol 15 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Yuri Koutcherov ◽  
Juergen K. Mai ◽  
Ken W. Ashwell ◽  
George Paxinos
Keyword(s):  
Hypothalamic Nucleus ◽  
Dorsomedial Hypothalamic Nucleus

Possible inhibitory role of prolactin-releasing peptide for ACTH release associated with running stress

2007 ◽  
Vol 292 (1) ◽  
pp. R497-R504 ◽  
Author(s):  
Nao Ohiwa ◽  
Hyukki Chang ◽  
Tsuyoshi Saito ◽  
Tatsushi Onaka ◽  
Takahiko Fujikawa ◽  
...  
Keyword(s):  
Blood Lactate ◽  
Metabolic Response ◽  
Ventrolateral Medulla ◽  
Hypothalamic Nucleus ◽  
Dependent Manner ◽  
Lactate Accumulation ◽  
Blood Lactate Accumulation ◽  
Prolactin Releasing Peptide ◽  
Acth Release

Exercise around the lactate threshold induces a stress response, defined as “running stress.” We have previously demonstrated that running stress is associated with activation of certain regions of the brain, e.g., the paraventricular hypothalamic nucleus (PVN) and supraoptic nucleus, that are hypothesized to play an integral role in regulating stress-related responses, including ACTH release during running. Thus we investigated the role of prolactin-releasing peptide (PrRP), found in the ventrolateral medulla and the nucleus of the solitary tract, which is known to project to the PVN during running-induced ACTH release. Accumulation of c-Fos in PrRP neurons correlated with running speeds, reaching maximal levels under running stress. Intracerebroventricular injection of neutralizing anti-PrRP antibodies led to increased plasma ACTH level and blood lactate accumulation during running stress, but not during restraint stress. Exogenous intracerebroventricular administration of low doses of PrRP had the opposite effects. Therefore, our results suggest that, during running stress, PrRP-containing neurons are activated in an exercise intensity-dependent manner, and likewise the produced endogenous PrRP attenuates ACTH release and blood lactate accumulation during running stress. Here we provide a novel perspective on understanding of PrRP in the endocrine-metabolic response associated with running stress.

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