Role of perivascular adipose tissue in endothelial dysfunction of adipose triglyceride lipase-deficient mice

Nitric Oxide ◽  
2012 ◽  
Vol 27 ◽  
pp. S21
Author(s):  
Karoline Dörffel ◽  
Sarah Winkler ◽  
Günter Hämmerle ◽  
Rudolf Zechner ◽  
Alois Lametschwandtner ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Endothelial Dysfunction ◽  
Adipose Triglyceride Lipase ◽  
Perivascular Adipose Tissue ◽  
Triglyceride Lipase ◽  
Deficient Mice

scholarly journals Role of perivascular adipose tissue in endothelial dysfunction of adipose triglyceride lipase-deficient mice

2012 ◽  
Vol 13 (S1) ◽  
Author(s):  
Karoline Pail ◽  
Sarah Winkler ◽  
Gerald Wölkart ◽  
Günter Hämmerle ◽  
Rudolf Zechner ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Endothelial Dysfunction ◽  
Adipose Triglyceride Lipase ◽  
Perivascular Adipose Tissue ◽  
Triglyceride Lipase ◽  
Deficient Mice

scholarly journals Endothelial dysfunction in adipose triglyceride lipase deficiency: role of perivascular adipose tissue

2013 ◽  
Vol 14 (S1) ◽  
Author(s):  
Marion Mussbacher ◽  
Karoline Pail ◽  
Gerald Wölkart ◽  
Günter Hämmerle ◽  
Alois Lametschwandtner ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Endothelial Dysfunction ◽  
Adipose Triglyceride Lipase ◽  
Perivascular Adipose Tissue ◽  
Triglyceride Lipase

scholarly journals Role of Adipose Triglyceride Lipase and Lipolysis in the Regulation of Insulin Secretion: Study in β-Cell-Specific ATGL-Deficient Mice

2013 ◽  
Vol 37 ◽  
pp. S58 ◽  
Author(s):  
Camille Attané ◽  
Marie- Line Peyot ◽  
Shupei Wang ◽  
Grant A. Mitchell ◽  
Roxane Lussier ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Adipose Triglyceride Lipase ◽  
Β Cell ◽  
Triglyceride Lipase ◽  
Deficient Mice

scholarly journals Role of the ubiquitin–proteasome system in cardiac dysfunction of adipose triglyceride lipase-deficient mice

2014 ◽  
Vol 77 ◽  
pp. 11-19 ◽  
Author(s):  
Marion Mussbacher ◽  
Heike Stessel ◽  
Gerald Wölkart ◽  
Guenter Haemmerle ◽  
Rudolf Zechner ◽  
...  
Keyword(s):  
Cardiac Dysfunction ◽  
Ubiquitin Proteasome System ◽  
Adipose Triglyceride Lipase ◽  
Triglyceride Lipase ◽  
Ubiquitin Proteasome ◽  
Deficient Mice

Adipose extracellular signal-regulated kinase 2 protected from endothelial dysfunction and the oxidative stress of perivascular adipose tissue in obese mice

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
A Osaki ◽  
Y Sato ◽  
S Endo ◽  
K Ito ◽  
K Kagami ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
Endothelial Dysfunction ◽  
Mrna Levels ◽  
Obese Mice ◽  
Fatty Acid Binding ◽  
Perivascular Adipose Tissue ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal

Abstract Introduction Extracellular signal-regulated kinase (ERK) modulates differentiation and maturation of adipocyte and the hypertrophy and differentiation of adipocytes affected the vascular diseases in obese. Changes in characters of adipocytes could develope the oxidative stress and inflammations. Moreover, changes in perivascular adipose tissue (PVAT) could modulate vascular tonus in obesity. However, the role of adipose ERK2 in endothelial function and characters of PVAT in obese in vivo had not been clarified, yet. Purpose This study aims to elucidate the role of the adipose ERK2 in endothelial-dependent relaxation (EDR) in mice model of obesity. The role of PVAT in EDR was also assessed. Methods and results We created adipose-specific ERK2 knock out mice (AE2KO) by crossing fatty acid binding protein 4 Cre and ERK2 flox mice and fed them with normal diet (ND) or high fat/ high sucrose diet (HFHSD) for 24 weeks. AE2KO fed with HFHSD gained more weight and revealed the heterogeneity in sizes of adipocyte in subcutaneous fat (SF). Furthermore, the mRNA levels of lipoprotein lipase, hormone-sensitive lipase, and peroxisome proliferator-activated receptor γ, which was the master genes of adipocyte differentiation, were markedly down-regulated in SF. PVAT in AE2KO with HFHSD was markedly enlarged and the mRNA expression of inflammatory adipocytokines, such as IL-1β and leptin were up-regulated. Next, we assessed EDR by acetylcholine (ACh) -induced relaxation in aortic rings with or without PVAT. EDR without PVAT was modestly decreased in AE2KO with HFHSD compared with wild type mice (WT) with HFHSD. Aortic rings with PVAT increased EDR in WT with ND. PVAT modestly decreased EDR in WT with HFHSD and mostly eliminated EDR in AE2KO with HFHSD. To assess the contraction factors released from PVAT, the solutions incubated with PVAT (SIP) were transferred to the normal aortic rings. SIP from WT with HFHSD mildly increased vascular tone and SIP from AE2KO with HFHSD further increased it. Tempol, which was superoxide scavenger, restored endothelial dysfunction with PVAT and suppressed the contraction with SIP from AE2KO with HFHSD. Fluorescence intensity of dihydroethidium stain of aorta and PVAT, which indicated that aortic and adipose superoxide production were elevated in AE2KO with HFHSD, which were mostly eliminated with tempol. Conclusions Adipose ERK2 selectively modulated differentiation in SF, suppressed the aortic oxidative stress and protected from endothelial dysfunction in obese. Moreover, adipose ERK2 suppressed the hypertrophy, inflammation, and oxidative stress of PVAT in obese. The oxidative stress with the inflammation in PVAT released vasoconstriction factors, which contributed to endothelial dysfunction in obese mice. Figure 1 Funding Acknowledgement Type of funding source: None

LB-OR-5 THE ROLE OF ADIPOSE TRIGLYCERIDE LIPASE (ATGL) IN HUMAN ADIPOSE TISSUE LIPOLYSIS

2007 ◽  
Vol 8 (1) ◽  
pp. 228
Author(s):  
M. Schweiger ◽  
G. Schoiswohl ◽  
A. Lass ◽  
G. Haemmerle ◽  
R. Zechner ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Adipose Triglyceride Lipase ◽  
Triglyceride Lipase ◽  
Adipose Tissue Lipolysis

scholarly journals The role of adipose triglyceride lipase in lipid and glucose homeostasis: lessons from transgenic mice

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Michael J. Trites ◽  
Robin D. Clugston
Keyword(s):  
Adipose Tissue ◽  
Transgenic Mice ◽  
Glucose Homeostasis ◽  
Function Analysis ◽  
Whole Body ◽  
Adipose Triglyceride Lipase ◽  
Metabolic Demand ◽  
Triglyceride Lipase ◽  
Fat Reserves

AbstractThe ability of mammals to store and draw on fat reserves has been a driving force throughout evolution in an environment with intermittent nutrient availability. The discovery of adipose triglyceride lipase (ATGL) as a triglyceride lipase provided a heightened understanding of the mechanisms governing mobilization of fat reserves from adipose tissue. ATGL catalyses the initial step in adipose triglyceride lipolysis, working in concert with other enzymes to mobilize triglyceride for energy production. In addition to the role of ATGL in adipose tissue triglyceride mobilization, ATGL plays crucial roles in regulating lipid homeostasis in other tissues. These roles have been characterized primarily using transgenic mice with tissue-specific ATGL ablation. For example, the global ATGL knockout induces a severe cardiac defect that results in premature mortality that is mimicked by inducible cardiomyocyte-specific ATGL knockout. Global- and adipose-specific ATGL ablation induces a whole-body shift from lipid metabolism to glucose metabolism to satisfy metabolic demand primarily facilitated by an increase in glucose uptake by skeletal muscle. Generation of liver-specific ATGL knockouts has implicated hepatic lipolysis as a critical component of normal liver function. Analysis of β-cell ATGL knockouts implicates the necessity of pancreatic ATGL in insulin secretion. The objective of this review is to discuss the contributions of ATGL to systemic lipid- and glucose-homeostasis discovered through the study of transgenic mice.

scholarly journals Association of Gut Hormones and Microbiota with Vascular Dysfunction in Obesity

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 613
Author(s):  
Valentina Rovella ◽  
Giuseppe Rodia ◽  
Francesca Di Daniele ◽  
Carmine Cardillo ◽  
Umberto Campia ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Endothelial Dysfunction ◽  
Gut Microbiota ◽  
Vascular Function ◽  
Vascular Reactivity ◽  
Metabolic Diseases ◽  
Gut Hormones ◽  
Perivascular Adipose Tissue ◽  
Complex Mechanisms

In the past few decades, obesity has reached pandemic proportions. Obesity is among the main risk factors for cardiovascular diseases, since chronic fat accumulation leads to dysfunction in vascular endothelium and to a precocious arterial stiffness. So far, not all the mechanisms linking adipose tissue and vascular reactivity have been explained. Recently, novel findings reported interesting pathological link between endothelial dysfunction with gut hormones and gut microbiota and energy homeostasis. These findings suggest an active role of gut secretome in regulating the mediators of vascular function, such as nitric oxide (NO) and endothelin-1 (ET-1) that need to be further investigated. Moreover, a central role of brain has been suggested as a main player in the regulation of the different factors and hormones beyond these complex mechanisms. The aim of the present review is to discuss the state of the art in this field, by focusing on the processes leading to endothelial dysfunction mediated by obesity and metabolic diseases, such as insulin resistance. The role of perivascular adipose tissue (PVAT), gut hormones, gut microbiota dysbiosis, and the CNS function in controlling satiety have been considered. Further understanding the crosstalk between these complex mechanisms will allow us to better design novel strategies for the prevention of obesity and its complications.

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