scholarly journals Serum zonulin measured by enzyme-linked immunosorbent assay may not be a reliable marker of small intestinal permeability in healthy adults

2020 ◽  
Vol 78 ◽  
pp. 82-92 ◽  
Author(s):  
Oana A. Tatucu-Babet ◽  
Adrienne Forsyth ◽  
Emma Owen ◽  
Diana Navarro-Perez ◽  
Jessica Radcliffe ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Immunosorbent Assay ◽  
Healthy Adults ◽  
Enzyme Linked Immunosorbent Assay ◽  
Small Intestinal ◽  
Reliable Marker

scholarly journals A Rapid Enzyme-Linked Immunosorbent Assay for Serum Amyloid a Using Sequence-Specific Antibodies

1993 ◽  
Vol 30 (3) ◽  
pp. 278-286 ◽  
Author(s):  
Martin-Tino Časl ◽  
Anders Grubb
Keyword(s):  
Immunosorbent Assay ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Microtitre Plate ◽  
Amyloid A ◽  
Diagnostic Potential ◽  
Assay Time ◽  
Reliable Marker ◽  
Specific Rabbit

A microtitre plate based enzyme-linked immunosorbent assay for determining the concentration of serum amyloid A (SAA) is described. The method employs easily produced sequence-specific rabbit antibodies and the preferential absorption of SAA to polystyrene, which obviates the use of capture antibodies and allows an assay time of only 3 · 5 h, so that the diagnostic potential of the SAA level as a rapid and reliable marker for inflammation can be fully exploited. The assay has a working concentration range of 0·1–2500 mg/L, which embraces the known biological variation of the SAA concentration. The intra-assay coefficient of variation (CV) for SAA concentrations above 10 mg/L is between 1 · 6 and 3·3% and the inter-assay CV between 3·0 and 4·2%. Recovery of SAA added to serum is from 96 to 102%.

scholarly journals Anti-Helicobacter pylori IgG in Asymptomatic Population

2016 ◽  
Vol 2 (2) ◽  
pp. 31-34
Author(s):  
Asma Azad Sumona ◽  
Md. Akram Hossain ◽  
AKM Shamsuzzaman ◽  
AKM Musa ◽  
Md. Chand Mahmud ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Immunosorbent Assay ◽  
Healthy Adults ◽  
Enzyme Linked Immunosorbent Assay ◽  
Seropositivity Rate ◽  
Medical College ◽  
H Pylori ◽  
The Impact ◽  
Sera Samples

To evaluate the serologic (IgG) response to Helicobacter pylori in asymptomatic population, a total of 45 asymptomatic healthy adults aged 18-65 years were included in the study. The study was carried out in the department of Microbiology, Mymensingh Medical College between July, 2006 to June, 2007. The sera samples of the participants were tested for presence of anti-H. pylori IgG by using Enzyme Linked Immunosorbent Assay (ELISA). Seropositivity rate for anti-H. pylori IgG in asymptomatic adults was found 88.89% at a cut-off level of 5 arbU/ml. The impact of changing cut-off level for IgG seropositivity was also examined in the present study. The seropositivity rate for anti-H. pylori IgG at 10 arbU/ml was found 71.11%.Bangladesh J Med Microbiol 2008; 02 (02): 31-34

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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