Printing orderly patterns on the insulating collector is the key for the development and application of flexible electronics. However, electrospinning on the insulating collector still has the problem of unstable jet due to the charge accumulation. The alternating current (AC)-induced electrohydrodynamic direct-writing (EDW) technology is a good way to decrease the interferences of charge repulsion, which is beneficial to printing orderly micro/nanostructures on the insulating collector. In this work, the sinusoidal AC-induced EDW is used to enhance the stability of charged jet and the deposition behaviors under AC electric field are also studied. The reciprocation transferring of charges induced by the AC electric field decreased the density of the accumulating charges on the insulating collector. The effect of AC electric field parameters on the direct-written micro/nanostructures are investigated to optimize the printing process. As the voltage peak increases, the fiber deposition bandwidth shows a trend of decreasing first and then increasing. Increasing the voltage frequency appropriately is beneficial to decrease the bandwidth of fiber deposition and to increase the stability of the jet. By improving the stability and controllability of the jet printing process, precise micro/nanopatterns can be direct-written on the insulating collector. This research provides a good foundation for expanding the application fields of EDW.