Confronting high-throughput protein refolding using high pressure and solution screens

2007 ◽  
Vol 55 (2) ◽  
pp. 209-224 ◽  
Author(s):  
M. Walid Qoronfleh ◽  
Lyndal K. Hesterberg ◽  
Matthew B. Seefeldt
Keyword(s):  
High Pressure ◽  
High Throughput ◽  
Protein Refolding

The new Material Science Powder Diffraction beamline at ALBA Synchrotron

2013 ◽  
Vol 28 (S2) ◽  
pp. S360-S370 ◽  
Author(s):  
F. Fauth ◽  
I. Peral ◽  
C. Popescu ◽  
M. Knapp
Keyword(s):  
High Pressure ◽  
High Resolution ◽  
Powder Diffraction ◽  
High Throughput ◽  
Material Science ◽  
In Series ◽  
New Material ◽  
Current Report

The current report describes the installation and the preliminary commissioning of the Material Science Powder Diffraction (MSPD) beamline at the Spanish synchrotron ALBA-CELLS. The beamline is fully dedicated to powder diffraction techniques and consists of two experimental stations positioned in series: a High Pressure/Microdiffraction station and a High Resolution/High Throughput powder diffraction station.

scholarly journals Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY

2004 ◽  
Vol 48 (3) ◽  
pp. 897-902 ◽  
Author(s):  
Thérèse Stachyra ◽  
Christophe Dini ◽  
Paul Ferrari ◽  
Ahmed Bouhss ◽  
Jean van Heijenoort ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Pressure ◽  
Cell Wall ◽  
High Throughput ◽  
High Throughput Screening ◽  
Reaction Medium ◽  
The Other ◽  
Functional Assay ◽  
Liquid Chromatography Separation ◽  
Fluorescent Derivative

ABSTRACT We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using UDP-MurNAc-Nε -dansylpentapeptide, a fluorescent derivative of the MraY nucleotide substrate, and a partially purified preparation of MraY solubilized from membranes of an Escherichia coli overproducing strain. Two versions of the assay were developed, one consisting of the high-pressure liquid chromatography separation of the substrate and product (dansylated lipid I) and the other, without separation and adapted to the high-throughput format, taking advantage of the different fluorescence properties of the nucleotide and lipid I in the reaction medium. The latter assay was validated with a set of natural and synthetic MraY inhibitors.

High-throughput method for a kinetics analysis of the high-pressure inactivation of microorganisms using microplates

2012 ◽  
Vol 113 (6) ◽  
pp. 788-791 ◽  
Author(s):  
Toshimi Hasegawa ◽  
Manabu Hayashi ◽  
Kazuki Nomura ◽  
Mayumi Hayashi ◽  
Miyuki Kido ◽  
...  
Keyword(s):  
High Pressure ◽  
High Throughput ◽  
Kinetics Analysis ◽  
High Throughput Method

High-throughput screening under demanding conditions: Cu/ZnO catalysts in high pressure methanol synthesis as an example

2003 ◽  
Vol 216 (1-2) ◽  
pp. 110-119 ◽  
Author(s):  
C. Kiener ◽  
M. Kurtz ◽  
H. Wilmer ◽  
C. Hoffmann ◽  
H.-W. Schmidt ◽  
...  
Keyword(s):  
High Pressure ◽  
High Throughput ◽  
Methanol Synthesis ◽  
High Throughput Screening

A continuous-flow, high-throughput, high-pressure parahydrogen converter for hyperpolarization in a clinical setting

2012 ◽  
Vol 26 (2) ◽  
pp. 124-131 ◽  
Author(s):  
Jan-Bernd Hövener ◽  
Sébastien Bär ◽  
Jochen Leupold ◽  
Klaus Jenne ◽  
Dieter Leibfritz ◽  
...  
Keyword(s):  
High Pressure ◽  
High Throughput ◽  
Clinical Setting ◽  
Continuous Flow
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