Bacillus subtilis HSY21 can reduce soybean root rot and inhibit the expression of genes related to the pathogenicity of Fusarium oxysporum

2021 ◽  
pp. 104916
Author(s):  
Songyang Han ◽  
Jiaxin Chen ◽  
Yujie Zhao ◽  
Hongsheng Cai ◽  
Changhong Guo
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Root Rot ◽  
Soybean Root ◽  
Expression Of Genes

Antagonistic Mechanism of Fusarium Oxysporum of Soybean Root Rot by Bacillus Subtilis

2011 ◽  
Vol 108 ◽  
pp. 127-131
Author(s):  
Yong Gang Li ◽  
Feng Ming Ma
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Root Rot ◽  
Mycelial Growth ◽  
Metabolic Product ◽  
Soybean Root ◽  
Activity Effect ◽  
Soybean Roots

. Fusarium oxysporum is a soil-borne fungus that infects soybean roots and causes soybean root rot, a widespread and destructive soybean disease. The potential strain X6 belonged to Bacillus subtilis for controlling soybean root rot. And antagonistic mechanism of the pathogenic Fusarium oxysporum of soybean root rot by B. subtilis X6 was investigated. The antagonistic mechanism of strain X6 againt F. oxysporum can effectively inhibit mycelial growth, spores bearing and germination. After treated with metabolic product of strain X6, protoplast from the hyphae became abnormal. The activity effect of metabolic product of strain X6 was sensitive to temperature. So the study lay the groundwork for further research and application.

Development of formulations of biological agents for management of root rot of lettuce and cucumber

2000 ◽  
Vol 46 (9) ◽  
pp. 809-816 ◽  
Author(s):  
G A Amer ◽  
R S Utkhede
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Fusarium Oxysporum ◽  
Wheat Bran ◽  
Root Rot ◽  
Room Temperature ◽  
Pythium Aphanidermatum ◽  
Growth And Yield ◽  
Peat Moss ◽  
Subtilis Strain

The effect of various carrier formulations of Bacillus subtilis and Pseudomonas putida were tested on germination, growth, and yield of lettuce and cucumber crops in the presence of Pythium aphanidermatum and Fusarium oxysporum f.sp. cucurbitacearum, respectively. Survival of B. subtilis and P. putida in various carriers under refrigeration (about 0°C) and at room temperature (about 22°C) was also studied. In all carrier formulations, B. subtilis strain BACT-0 survived up to 45 days. After 45 days of storage at room temperature (about 22°C), populations B. subtilis strain BACT-0 were significantly higher in vermiculite, kaolin, and bacterial broth carriers compared with other carriers. Populations of P. putida were significantly higher in vermiculite, peat moss, wheat bran, and bacterial broth than in other carriers when stored either under refrigeration (about 0°C) or at room temperature (about 22°C) for 15 or 45 days. Germination of lettuce seed was not affected in vermiculite, talc, kaolin, and peat moss carriers, but germination was significantly reduced in alginate and bacterial broth carriers of B. subtilis compared to the non-treated control. Germination of cucumber seed was not affected by any of the carriers. Significantly higher fresh lettuce and root weights were observed in vermiculite and kaolin carriers of B. subtilis compared with P. aphanidermatum-inoculated control plants. Lettuce treated with vermiculite, and kaolin carriers of B. subtilis, or non-inoculated control lettuce plants had significantly lower root rot ratings than talc, peat moss, bacterial broth, and P. aphanidermatum-inoculated control plants. Growth and yield of cucumber plants were significantly higher in vermiculite-based carrier of P. putida than the other carriers and Fusarium oxysporum f.sp. cucurbitacearum-inoculated plants.Key words: Bacillus subtilis, Pseudomonas putida, Fusarium oxysporum f.sp. cucurbitacearum, Pythium aphanidermatum, talc, vermiculite, alginate, kaolin, peat moss, wheat bran, oat bran.

scholarly journals Evaluation of Seed and Soil Treatments with Novel Bacillus subtilis Strains for Control of Soybean Root Rot Caused by Fusarium oxysporum and F. graminearum

Plant Disease ◽  
2009 ◽  
Vol 93 (12) ◽  
pp. 1317-1323 ◽  
Author(s):  
J. X. Zhang ◽  
A. G. Xue ◽  
J. T. Tambong
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Plant Height ◽  
Root Rot ◽  
Mycelial Growth ◽  
Dry Weight ◽  
Fusarium Root Rot ◽  
Root Rots ◽  
Root Dry Weight ◽  
Corn Roots

Fusarium root rot is an important disease of soybean in Ontario, Canada. This study is to select antagonistic bacterial agents as effective alternatives to chemical pesticides for the control of root rots caused by Fusarium oxysporum and F. graminearum. Twenty-two Bacillus subtilis strains from soybean and corn roots were tested in dual cultures for inhibition of mycelial growth of F. oxysporum and F. graminearum. All strains significantly reduced mycelial growth of F. oxysporum by approximately 17 to 48% and of F. graminearum by 10 to 32%. Ten B. subtilis strains selected based on their larger fungal inhibition zones were evaluated against macroconidial germination. These strains inhibited the spore germination of F. oxysporum by 20 to 48% and of F. graminearum by 14 to 32% in cell-free filtrates. Under greenhouse conditions, the efficacy of seed and soil treatments with B. subtilis strains against the two Fusarium root rot pathogens was evaluated based on root rot severity, seedling emergence, plant height, and root dry weight. Six B. subtilis strains (SB01, SB04, SB23, SB24, SB28, and SB33) from soybean roots and two strains (CB01 and CH22) from corn roots significantly reduced the severity of the two Fusarium root rots in seed or soil treatments. Strains SB01, SB04, SB23, and SB24 were the most effective treatments against both pathogens in either seed or soil treatment. When applied as seed treatments, these four strains reduced root rot severity by 43 to 63% and increased emergence by 13 to 17%, plant height by 9 to 18%, and root dry weight by 8.4 to 19%. When used as soil treatments, they reduced root rot severity by 68 to 74% and increased emergence by 14 to 18%, plant height by 11 to 23%, and root dry weight by 16 to 24%. These results suggest that the novel strains of B. subtilis identified in this research can be effective alternatives to fungicides in managing Fusarium root rots of soybean, and a greater level of efficacy may be achieved when they were used as soil treatments than seed treatments.

scholarly journals First Report of Fusarium oxysporum Causing Root Rot in Ophiopogon bodinieri in China

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1254
Author(s):  
B. H. Lu ◽  
Z. Wang ◽  
G. J. Yi ◽  
G. W. Tan ◽  
F. Zeng ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Root Rot ◽  
First Report

scholarly journals DNA repair and the evolution of transformation in Bacillus subtilis. II. Role of inducible repair.

Genetics ◽  
1989 ◽  
Vol 121 (3) ◽  
pp. 411-422
Author(s):  
M F Wojciechowski ◽  
M A Hoelzer ◽  
R E Michod
Keyword(s):  
Dna Repair ◽  
Bacillus Subtilis ◽  
Plasmid Dna ◽  
Excision Repair ◽  
Physiological State ◽  
Transformation Rate ◽  
Exogenous Dna ◽  
Expression Of Genes ◽  
Cell Subpopulations ◽  
Experimental Treatments

Abstract In Bacillus subtilis, DNA repair and recombination are intimately associated with competence, the physiological state in which the bacterium can bind, take up and recombine exogenous DNA. Previously, we have shown that the homologous DNA transformation rate (ratio of transformants to total cells) increases with increasing UV dosage if cells are transformed after exposure to UV radiation (UV-DNA), whereas the transformation rate decreases if cells are transformed before exposure to UV (DNA-UV). In this report, by using different DNA repair-deficient mutants, we show that the greater increase in transformation rate in UV-DNA experiments than in DNA-UV experiments does not depend upon excision repair or inducible SOS-like repair, although certain quantitative aspects of the response do depend upon these repair systems. We also show that there is no increase in the transformation rate in a UV-DNA experiment when repair and recombination proficient cells are transformed with nonhomologous plasmid DNA, although the results in a DNA-UV experiment are essentially unchanged by using plasmid DNA. We have used din operon fusions as a sensitive means of assaying for the expression of genes under the control of the SOS-like regulon in both competent and noncompetent cell subpopulations as a consequence of competence development and our subsequent experimental treatments. Results indicate that the SOS-like system is induced in both competent and noncompetent subpopulations in our treatments and so should not be a major factor in the differential response in transformation rate observed in UV-DNA and DNA-UV treatments. These results provide further support to the hypothesis that the evolutionary function of competence is to bring DNA into the cell for use as template in the repair of DNA damage.

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

scholarly journals Intracellular localization of the mycobacterial stressosome complex

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Malavika Ramesh ◽  
Ram Gopal Nitharwal ◽  
Phani Rama Krishna Behra ◽  
B. M. Fredrik Pettersson ◽  
Santanu Dasgupta ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fluorescence Microscopy ◽  
Bacillus Cereus ◽  
Intracellular Localization ◽  
Sigma Factors ◽  
Alternative Sigma Factors ◽  
Expression Of Genes ◽  
Molecular Machinery ◽  
Stress Signals ◽  
Activation Pathways

AbstractMicroorganisms survive stresses by alternating the expression of genes suitable for surviving the immediate and present danger and eventually adapt to new conditions. Many bacteria have evolved a multiprotein "molecular machinery" designated the "Stressosome" that integrates different stress signals and activates alternative sigma factors for appropriate downstream responses. We and others have identified orthologs of some of the Bacillus subtilis stressosome components, RsbR, RsbS, RsbT and RsbUVW in several mycobacteria and we have previously reported mutual interactions among the stressosome components RsbR, RsbS, RsbT and RsbUVW from Mycobacterium marinum. Here we provide evidence that "STAS" domains of both RsbR and RsbS are important for establishing the interaction and thus critical for stressosome assembly. Fluorescence microscopy further suggested co-localization of RsbR and RsbS in multiprotein complexes visible as co-localized fluorescent foci distributed at scattered locations in the M. marinum cytoplasm; the number, intensity and distribution of such foci changed in cells under stressed conditions. Finally, we provide bioinformatics data that 17 (of 244) mycobacteria, which lack the RsbRST genes, carry homologs of Bacillus cereus genes rsbK and rsbM indicating the existence of alternative σF activation pathways among mycobacteria.

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