scholarly journals Evaluation of Seed and Soil Treatments with Novel Bacillus subtilis Strains for Control of Soybean Root Rot Caused by Fusarium oxysporum and F. graminearum

Plant Disease ◽  
2009 ◽  
Vol 93 (12) ◽  
pp. 1317-1323 ◽  
Author(s):  
J. X. Zhang ◽  
A. G. Xue ◽  
J. T. Tambong
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Plant Height ◽  
Root Rot ◽  
Mycelial Growth ◽  
Dry Weight ◽  
Fusarium Root Rot ◽  
Root Rots ◽  
Root Dry Weight ◽  
Corn Roots

Fusarium root rot is an important disease of soybean in Ontario, Canada. This study is to select antagonistic bacterial agents as effective alternatives to chemical pesticides for the control of root rots caused by Fusarium oxysporum and F. graminearum. Twenty-two Bacillus subtilis strains from soybean and corn roots were tested in dual cultures for inhibition of mycelial growth of F. oxysporum and F. graminearum. All strains significantly reduced mycelial growth of F. oxysporum by approximately 17 to 48% and of F. graminearum by 10 to 32%. Ten B. subtilis strains selected based on their larger fungal inhibition zones were evaluated against macroconidial germination. These strains inhibited the spore germination of F. oxysporum by 20 to 48% and of F. graminearum by 14 to 32% in cell-free filtrates. Under greenhouse conditions, the efficacy of seed and soil treatments with B. subtilis strains against the two Fusarium root rot pathogens was evaluated based on root rot severity, seedling emergence, plant height, and root dry weight. Six B. subtilis strains (SB01, SB04, SB23, SB24, SB28, and SB33) from soybean roots and two strains (CB01 and CH22) from corn roots significantly reduced the severity of the two Fusarium root rots in seed or soil treatments. Strains SB01, SB04, SB23, and SB24 were the most effective treatments against both pathogens in either seed or soil treatment. When applied as seed treatments, these four strains reduced root rot severity by 43 to 63% and increased emergence by 13 to 17%, plant height by 9 to 18%, and root dry weight by 8.4 to 19%. When used as soil treatments, they reduced root rot severity by 68 to 74% and increased emergence by 14 to 18%, plant height by 11 to 23%, and root dry weight by 16 to 24%. These results suggest that the novel strains of B. subtilis identified in this research can be effective alternatives to fungicides in managing Fusarium root rots of soybean, and a greater level of efficacy may be achieved when they were used as soil treatments than seed treatments.

Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot

2013 ◽  
Vol 93 (2) ◽  
pp. 221-236 ◽  
Author(s):  
J. X. Zhang ◽  
A. G. Xue ◽  
E. R. Cober ◽  
M. J. Morrison ◽  
H. J. Zhang ◽  
...  
Keyword(s):  
Plant Height ◽  
Root Rot ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Field Resistance ◽  
Adult Plant ◽  
Cultivar Resistance ◽  
Soybean Root ◽  
Root Dry Weight ◽  
Seedling Roots

Zhang, J. X., Xue, A. G., Cober, E. R., Morrison, M. J., Zhang, H. J., Zhang, S. Z. and Gregorich, E. 2013. Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot. Can. J. Plant Sci. 93: 221–236. Root rot complex, caused by Fusarium and Rhizoctonia species, is a major soybean disease in Canada. We isolated nine Fusarium and Rhizoctonia species including F. oxysporum (Fo), F. graminearum (Fg), F. solani (Fs), F. avenaceum (Fa), F. tricinctum (Ft), F. sporotrichioides (Fsp), F. equiseti (Fe), F. poae (Fp), and R. solani (Rs) from soybean roots in eastern Ontario, Canada. The isolation results indicated that Fo was the most prevalent species while Fa, Fsp, and Fp were the least frequent species in the soybean rhizosphere. Numbers of Fo, Fs, Fg, and Rs isolates recovered from adult plant roots were significantly greater than those from seedling roots (P<0.01). The Rs, Fg and Fsp isolates were significantly more abundant in the no-till field than in the tilled field (P<0.01). Based on the greenhouse assays, Rs, Fg, and Fa were the most pathogenic species, while Fe and Fsp were the least pathogenic to soybean. The field resistance evaluation, based on the root rot severity, identified 21, 17, 30, and 3 out of 70 cultivars among the most tolerant to Fg, Fo, Fs, and Rs, respectively. A few of the cultivars showed partial resistance to multiple species, based on root rot severity and reduction in their seedling emergence, plant height, and root dry weight, but no cultivar was found to partially resist all four species. There was no correlation (P>0.05) between root rot severity and the reduction in seedling emergence, plant height, or root dry weight.

scholarly journals Influence of MCPA on Fusarium oxysporum root rot and red clover growth under controlled greenhouse conditions

2006 ◽  
Vol 87 (1) ◽  
pp. 9-15
Author(s):  
Ricardo Ceballos ◽  
Graciela Palma ◽  
Fernando Perich ◽  
Fernando Pardo ◽  
Andrés Quiroz
Keyword(s):  
Fusarium Oxysporum ◽  
Root Rot ◽  
Mycelial Growth ◽  
Red Clover ◽  
Dry Weight ◽  
Conidial Germination ◽  
High Rate ◽  
Crown Diameter ◽  
Shoot Dry Weight

Abstract Root rot caused by Fusarium oxysporum is a disease that reduces red clover persistence. Agronomical management of red clover includes MCPA application, and there is no information regarding the effects of this herbicide on the disease. MCPA was evaluated for its effects on F. oxysporum root rot and red clover (Trifolium pratense) growth in a greenhouse experiment. Additionally, in vitro mycelial growth and conidial germination of F. oxysporum were studied. For shoot dry weight and crown diameter of seedlings, the interaction of herbicide and inoculum was significant at 30 d. The herbicide–inoculum treatment reduced shoot dry weight by 20% at 1X rate and by 24% at 2X rate, and crown diameter was reduced by 10% at the high rate. The MCPA treatment caused a 40% reduction of root dry weight by the end of the experiment. Application of MCPA caused fusarium root rot to increase in severity on red clover seedlings and caused phytotoxicity at the high rate. Interaction with the other growth parameters was not significant, indicating that the effects of herbicide and inoculum were independent. Conidial germination and mycelial growth in vitro were reduced by MCPA. Results suggest that red clover growth could be negatively affected by F. oxysporum after MCPA application and that root rot severity increases at high rates of MCPA.

scholarly journals The Vegetative Growth Response of Detam Soybean Varieties towards Bacillus subtilis and Trichoderma sp. Applications as Bio-fertilizer

2021 ◽  
Vol 232 ◽  
pp. 03024
Author(s):  
Agus Miftakhurrohmat ◽  
Sutarman
Keyword(s):  
Bacillus Subtilis ◽  
Leaf Area ◽  
Plant Height ◽  
Dry Weight ◽  
Stem Diameter ◽  
Observation Data ◽  
Root Dry Weight ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Soybean Plants

This study aims to determine the effect of bacterial isolates of Bacillus subtilis Bs-Sdj-01 and Trichoderma sp. Tc-Jro-02 isolates as biological fertilizer in plant growth until the end of the vegetative phase. The experiment was arranged factorially in a Completely Randomized Design (CRD) with each factor being the application of B. subtilis consisting of and without B. subtilis and the Trichoderma application consisting of with and without Trichoderma; the experiment was repeated four times. The observed variables were: plant height, stem diameter, number of leaves, leaf area, stover dry weight, root dry weight, and B. subtilis population at the end of the observation. Data were analyzed using with ANOVA and HSD tests at the 5% level. The combination of Trichoderma sp. and B. subtilis bacteria produce the highest increase in plant height, stem diameter, number of leaves, leaf area, stover dry weight, and root dry weight of soybean plants up to 35 days after planting and increase B. subtilis population grew from 1010 CFU.g-1 to be 4.43x1011 CFU.g-1. The presence of Trichoderma supports the activity of B. subtilis in the rhizosphere of the Detam variety soybean plantation.

Antagonistic Mechanism of Fusarium Oxysporum of Soybean Root Rot by Bacillus Subtilis

2011 ◽  
Vol 108 ◽  
pp. 127-131
Author(s):  
Yong Gang Li ◽  
Feng Ming Ma
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Oxysporum ◽  
Root Rot ◽  
Mycelial Growth ◽  
Metabolic Product ◽  
Soybean Root ◽  
Activity Effect ◽  
Soybean Roots

. Fusarium oxysporum is a soil-borne fungus that infects soybean roots and causes soybean root rot, a widespread and destructive soybean disease. The potential strain X6 belonged to Bacillus subtilis for controlling soybean root rot. And antagonistic mechanism of the pathogenic Fusarium oxysporum of soybean root rot by B. subtilis X6 was investigated. The antagonistic mechanism of strain X6 againt F. oxysporum can effectively inhibit mycelial growth, spores bearing and germination. After treated with metabolic product of strain X6, protoplast from the hyphae became abnormal. The activity effect of metabolic product of strain X6 was sensitive to temperature. So the study lay the groundwork for further research and application.

scholarly journals Rhizobacteria inoculation in maize associated with nitrogen and zinc fertilization at sowing

2021 ◽  
Vol 25 (2) ◽  
pp. 96-100
Author(s):  
Andréia de L. Moreno ◽  
Jorge F. Kusdra ◽  
Angelita A. C. Picazevicz
Keyword(s):  
Bacillus Subtilis ◽  
Nitrogen Content ◽  
Plant Height ◽  
Nitrogen Fertilization ◽  
Azospirillum Brasilense ◽  
Dry Weight ◽  
Stem Diameter ◽  
Wide Range ◽  
Maize Plants ◽  
Total Dry Weight

ABSTRACT Rhizobacteria have a wide range of plant growth-promoting mechanisms of action, making them an alternative and/or complementary biological input for chemical fertilizers. In this respect, the present study aimed to assess growth and nitrogen accumulation in maize plants as a function of Azospirillum brasilense, Bacillus subtilis, zinc and nitrogen application at sowing. The experiment with the ‘AL Bandeirante’ maize variety was conducted in a greenhouse, using a completely randomized design. The treatments were arranged in a 2 x 2 x 2 x 2 factorial scheme, with six repetitions, considering the presence and absence of Azospirillum brasilense (5 g kg-1), Bacillus subtilis (5 mL kg-1) and zinc (20 g kg-1) in the seeds and addition or not of nitrogen (30 kg ha-1) to the soil. The variables assessed were plant height, stem diameter, leaf, stem, shoot, root and total dry weight, and shoot nitrogen content. Nitrogen fertilization in the absence of zinc increased shoot and total dry weight as well as shoot nitrogen content. Zinc applied to the seeds improved the total and stem dry weight of maize plants in the absence of Bacillus subtilis. Inoculation with Azospirillum brasilense and Bacillus subtilis increased the stem diameter and shoot nitrogen content of maize plants when nitrogen fertilization was not performed at sowing. There was no isolated or interaction effect between factors for plant height and root dry weight.

scholarly journals THE GROWTH RATE OF CELERY (Apium graveolens L.) AT VARIOUS COMPOSITIONS OF ORGANIC AND SYNTHETIC FERTILIZER

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Ghani Hendrika ◽  
Arifah Rahayu ◽  
Yanyan Mulyaningsih
Keyword(s):  
Plant Height ◽  
Dry Weight ◽  
Apium Graveolens ◽  
Fresh Weight ◽  
Higher Plant ◽  
Root Dry Weight ◽  
Synthetic Fertilizer ◽  
K Fertilizer ◽  
Compost Plant ◽  
Number Of Leaves

This study was aimed at assessing the growth and production of celery plants grown with various compositions of N,P and K fertilizer and compost. The study was done at the Trial Farm of Faculty of Agriculture, Djuanda University, Bogor fiom September to November 2015. A completely randomized design with two factors was used. The factors consisted of compost rate and N,P and K fertilizer rate. Compost was given in four levels, namely no compost (0% R), 26.3% gcompost/plant (50% R), 52.5 g compost/plant (100% R), and 78.7 g compost/plant (150% R). N, P and K fertilizer was given in five levels, namely no fertilizer (0% R), 4 g Urea, 0.3 g SP-36, 0.2 g KCl (25% R), 0.9 g Urea, 0.6 g SP-36, 0.4 g KCI (50% R), 1.3 g Urea, 0.8 g SP-36, 0.6 g KCl (75% R), and 1.7 g Urea, 1.1 g SP-36, 0.8 g KCl (100% R). All fertilizers were administered gradually at the time of planting and 2 weeks after planting. Three replicates were allocated into each treatment making up 60 experimental units. Results showed that celery plants given synthetic fertilizer at 100% R had higher plant height, number of leaves, number of seedlings, harvest fresh weight (g), saleable fresh weight (g), root biomass (g), and root dry weight (g) than those treated with no fertilizer (0% R). Plants treated with 150% compost had higher plant height, number of leaves, harvest fresh weight (g), saleable fresh weight (g), root length (cm), and root dry weight (g) than those treated with no fertilizer (0% R). Thus the best celery growth and production in this study was obtained on plant 150% R compost   and 100% R synthetic fertilizer nourished.Key words: celery, compost, N, P, K fertilizer, harvest flesh weight

scholarly journals The Effect of Biochars and Endophytic Bacteria on Growth and Root Rot Disease Incidence of Fusarium Infested Narrow-Leafed Lupin (Lupinus angustifolius L.)

2020 ◽  
Vol 8 (4) ◽  
pp. 496
Author(s):  
Dilfuza Egamberdieva ◽  
Vyacheslav Shurigin ◽  
Burak Alaylar ◽  
Hua Ma ◽  
Marina E. H. Müller ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Rot ◽  
Endophytic Bacteria ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Microbial Interactions ◽  
Root Rot Disease ◽  
Root Dry Weight ◽  
Rot Disease

The effects of biochar on plant growth vary depending on the applied biochar type, study site environmental conditions, microbial species, and plant–microbial interactions. The objectives of the present study were therefore to assess 1) the response of growth parameters of lupin and root disease incidence to the application of three biochar types in a loamy sandy soil, and 2) the role of endophytic bacteria in biological control of root rot disease incidence in lupin after the amendment of soil with different biochar types. As biochar types we tested (i) hydrochar (HTC) from maize silage, (ii) pyrolysis char from maize (MBC), and (iii) pyrolysis char from wood (WBC) at three different concentrations (1%, 2%, and 3% of char as soil amendments). There were no significant effects in lupin shoot and root growth in soils amended with WBC at any of the concentrations. MBC did not affect plant growth except for root dry weight at 2% MBC. HTC char at 2% concentration, significantly increased the root dry weight of lupin by 54–75%, and shoot dry weight by 21–25%. Lupin plants grown in soil amended with 2% and 3% WBC and MBC chars showed 40–50% and 10–20% disease symptoms, respectively. Plants grown in soil without biochar and with HTC char were healthy, and no disease incidence occurred. Pseudomonas putida L2 and Stenotrophomonas pavanii L8 isolates demonstrated a disease reduction compared to un-inoculated plants under MBC and WBC amended soil that was infested with Fusarium solani.

scholarly journals Evaluation of growth and yield of selected hybrid and inbred rice varieties grown in net-house during transplanted aman season

1970 ◽  
Vol 34 (1) ◽  
pp. 67-73
Author(s):  
M SH Islam ◽  
MSU Bhuiya ◽  
AR Gomosta ◽  
AR Sarkar ◽  
MM Hussain
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Dry Matter ◽  
Dry Weight ◽  
Growth And Yield ◽  
Rooting Depth ◽  
Rice Varieties ◽  
Root Dry Weight ◽  
Matter Production ◽  
1000 Grain Weight

Pot experiments were conducted during T. aman 2001 and 2002 (wet season) at Bangladesh Rice Research Institute (BRRI) in net house. Hybrid variety Sonarbangla-1 and inbred modern variety BRRI dhan-31 were used in both the seasons and BRRI hybrid dhan-l was used in 2002. The main objective of the experiments was to compare the growth and yield behaviour of hybrid and inbred rice varieties under controlled condition. In 2001, BRRI dhan-3l had about 10-15% higher plant height, very similar tillers/plant, 15-25% higher leaf area at all days after transplanting (DAT) compared to Sonarbangla-1. Sonarbangla- 1 had about 40% higher dry matter production at 25 DAT but had very similar dry matter production at 50 and 75 DAT, 4-11% higher rooting depth at all DATs, about 22% higher root dry weight at 25 DAT, but 5-10% lower root dry weight at 50 and 75 DAT compared to BRRI dhan-31. The photosynthetic rate was higher (20 μ mol m-2/sec-1) in BRRI dhan-3l at 35 DAT (maximum tillering stage) but at 65 DAT, Sonarbangla-l had higher photosynthetic rate of 19.5 μ mol m-2 sec-1. BRRI dhan-3l had higher panicles/plant than Sonarbangla-1, but Sonarbangla-1 had higher number of grains/panicle, 1000-grain weight and grain yield than BRRI dhan-31. In 2002, BRRI dhan-31 had the highest plant height at 25 DAT, but at 75 DAT, BRRI hybrid dhan-l had the highest plant height. Sonarbangla-1 had the largest leaf area at 25 and 50 DAT followed by BRRI dhan-31, but at 75 DAT, BRRI dhan-31 had the largest leaf area. The highest shoot dry matter was observed in BRRI dhan-31 followed by Sonarbangla-1 at all DATs. Sonarbangla-1 had the highest rooting depth and root dry weight at all DATs. BRRI dhan-31 gave the highest number of panicles/plant followed by Sonarbangla-I, BRRI hybrid dhan-l had the highest grains/panicle followed by BRRI dhan-31 and Sonarbangla-I had the highest 1000-grain weight followed by BRRI dhan-31. The highest amount of grains/plant (34.6 g) was obtained from BRRI dhan-31. Key Words: Shoot dry matter; root dry weight; leaf area; photosynthesis; grain yield. DOI: 10.3329/bjar.v34i1.5755Bangladesh J. Agril. Res. 34(1) : 67-73, March 2009

Effects of Biochar on Acid Black Soil Nutrient, Soybean Root and Yield

2012 ◽  
Vol 524-527 ◽  
pp. 2278-2289 ◽  
Author(s):  
Da Wei Yin ◽  
Jun Meng ◽  
Gui Ping Zheng ◽  
Xue Mei Zhong ◽  
Lan Yu ◽  
...  
Keyword(s):  
Plant Height ◽  
Lateral Root ◽  
Soil Nutrient ◽  
Dry Weight ◽  
Black Soil ◽  
Mature Stage ◽  
Root Number ◽  
Soybean Root ◽  
Root Dry Weight ◽  
Mature Period

This research focuses on studying the effects of biochar on acidic soil nutrient, soybean production, root and plant height,using the method of pot plant simulation for soybean cultivation patterns in three ridging test. Biochar was applied for pot with set 0, 1, 5, 10, 15, 20, 25t hm-2. Biochar, diammonium hydrogen phosphate, urea and potassium chloride were fully mixed then sampled on soybean clover stage (V3), full bloom stage (R2) and the early mature stage (R7).This research examined the soybean root index. At the early mature stage (R7), soil samples were blown dry and examined chemical index. During the mature period (R8), we examined the yield and plant height. The results showed that, with the increase of the content of the biochar, available potassium，and organic matter was increased significantly, increasing 77.69mgkg-1, and 7.56gkg-1 compared with control respectively. The root dry weight, main root length, lateral root number, total length, lateral root number, root dry weight of the maximum was respectively increased by 80.85%, 100%, 33.86%, 167.74%, 26.31% and 80.41% than control in early mature stage. The yield increased by 35.97% compared with control.

Development of formulations of biological agents for management of root rot of lettuce and cucumber

2000 ◽  
Vol 46 (9) ◽  
pp. 809-816 ◽  
Author(s):  
G A Amer ◽  
R S Utkhede
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Fusarium Oxysporum ◽  
Wheat Bran ◽  
Root Rot ◽  
Room Temperature ◽  
Pythium Aphanidermatum ◽  
Growth And Yield ◽  
Peat Moss ◽  
Subtilis Strain

The effect of various carrier formulations of Bacillus subtilis and Pseudomonas putida were tested on germination, growth, and yield of lettuce and cucumber crops in the presence of Pythium aphanidermatum and Fusarium oxysporum f.sp. cucurbitacearum, respectively. Survival of B. subtilis and P. putida in various carriers under refrigeration (about 0°C) and at room temperature (about 22°C) was also studied. In all carrier formulations, B. subtilis strain BACT-0 survived up to 45 days. After 45 days of storage at room temperature (about 22°C), populations B. subtilis strain BACT-0 were significantly higher in vermiculite, kaolin, and bacterial broth carriers compared with other carriers. Populations of P. putida were significantly higher in vermiculite, peat moss, wheat bran, and bacterial broth than in other carriers when stored either under refrigeration (about 0°C) or at room temperature (about 22°C) for 15 or 45 days. Germination of lettuce seed was not affected in vermiculite, talc, kaolin, and peat moss carriers, but germination was significantly reduced in alginate and bacterial broth carriers of B. subtilis compared to the non-treated control. Germination of cucumber seed was not affected by any of the carriers. Significantly higher fresh lettuce and root weights were observed in vermiculite and kaolin carriers of B. subtilis compared with P. aphanidermatum-inoculated control plants. Lettuce treated with vermiculite, and kaolin carriers of B. subtilis, or non-inoculated control lettuce plants had significantly lower root rot ratings than talc, peat moss, bacterial broth, and P. aphanidermatum-inoculated control plants. Growth and yield of cucumber plants were significantly higher in vermiculite-based carrier of P. putida than the other carriers and Fusarium oxysporum f.sp. cucurbitacearum-inoculated plants.Key words: Bacillus subtilis, Pseudomonas putida, Fusarium oxysporum f.sp. cucurbitacearum, Pythium aphanidermatum, talc, vermiculite, alginate, kaolin, peat moss, wheat bran, oat bran.

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