Pyrazinopyrimidine alkaloids from a mangrove-derived fungus Aspergillus versicolor HDN11-84

Fungi is known to produce a wide range of biologically active metabolites and enzymes. Enzymes produced by fungi are utilized in food and pharmaceutical industries because of their rich enzymatic profile. Filamentous fungi are particularly interesting due to their high production of extracellular enzymes which has a large industrial potential. The aim of this study is to isolate potential soil fungi species that are able to produce functional enzymes for industries. Five Aspergillus species were successfully isolated from antibiotic overexposed soil (GPS coordinate of N3.093219 E101.40269) by standard microbiological method. The isolated fungi were identified via morphological observations and molecular tools; polymerase chain reactions, ITS 1 (5’- TCC GTA GGT GAA CCT GCG G3’) forward primer and ITS 4 (5’-TCC TCC GCT TAT TGA TAT GC-3’) reverse primer. The isolated fungi were identified as Aspergillus sydowii strain SCAU066, Aspergillus tamarii isolate TN-7, Aspergillus candidus strain KUFA 0062, Aspergillus versicolor isolate BAB-6580, and Aspergillus protuberus strain KAS 6024. Supernatant obtained via submerged fermentation of the isolated fungi in potato dextrose broth (PDB) and extracted via centrifugation was loaded onto specific media to screen for the production of xylanolytic, cellulolytic and amylolytic enzymes. The present findings indicate that Aspergillus sydowii strain SCAU066 and Aspergillus versicolor isolate BAB-6580 have great potential as an alternative source of xylanolytic, cellulolytic and amylolytic enzymes.

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Metabolomics and Pharmacological Screening of Aspergillus versicolor Isolated from Hyrtios erectus Red Sea Sponge; Egypt

Current Bioactive Compounds ◽

10.2174/1573407215666191111122711 ◽

2020 ◽

Vol 16 (7) ◽

pp. 1083-1102

Author(s):

Mohamed A. Shreadah ◽

Nehad M.A. El Moneam ◽

Samy A. El-Assar ◽

Asmaa Nabil-Adam

Keyword(s):

Oxidative Stress ◽

Red Sea ◽

Crude Extract ◽

Quantitative Methods ◽

Total Phenolic ◽

Aspergillus Versicolor ◽

Crude Extracts ◽

Study Results ◽

Anti Inflammatory ◽

Pharmacological Screening

Background: Aspergillus Versicolor is a marine-derived fungus isolated from Hyrtios Erectus Red Sea sponge. Methods: The aim of this study was to carry out a pharmacological screening and investigation for the in vitro biological activity (antioxidant, cholinergic, antidiabetic and anticancer) of Aspergillus Versicolor crude extract’s active compounds by using different qualitative and quantitative methods. Results: The present study results showed that Aspergillus Versicolor crude extracts contain 0.6 mg total phenolic/mg crude extract. Aspergillus Versicolor also showed a potent antioxidative capacity by decreasing the oxidation of ABTS. The anticancer and inhibitory effects of Aspergillus Versicolor crude extracts on PTK and SHKI were found to be 75.29 % and 80.76%; respectively. The AChE inhibitory assay revealed that Aspergillus Versicolor extracts had an inhibitory percentage of 86.67%. Furthermore, the anti-inflammatory activity using COX1, COX2, TNF, and IL6 was 77.32, 85.21 %, 59.83%, and 56.15%; respectively. Additionally, the anti-viral effect using reverse transcriptase enzyme showed high antiviral activity with 92.10 %. Conclusion: The current study confirmed that the Aspergillus versicolor crude extract and its active constituents showed strong effects on diminishing the oxidative stress, neurodegenerative damage, antiinflammatory, anti-cancer and anti-viral, suggesting their beneficial role as a promising fermented product in the treatment of cancer, oxidative stress, Alzheimer's, anti-inflammatory and anti-viral diseases.

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Antioxidative phenolic compounds from a marine-derived fungus Aspergillus versicolor

Tetrahedron ◽

10.1016/j.tet.2015.10.038 ◽

2016 ◽

Vol 72 (1) ◽

pp. 50-57 ◽

Cited By ~ 32

Author(s):

Zehong Wu ◽

Yongrui Wang ◽

Dong Liu ◽

Peter Proksch ◽

Siwang Yu ◽

...

Keyword(s):

Phenolic Compounds ◽

Aspergillus Versicolor

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Antifungal activity of oregano extract against A. Versicolor, E. Nidulans and Eurotium spp.: Producers of sterigmatocystin

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1120165k ◽

2011 ◽

pp. 165-176 ◽

Cited By ~ 2

Author(s):

Suncica Kocic-Tanackov ◽

Gordana Dimic ◽

Ilija Tanackov ◽

Danijela Tuco

Keyword(s):

Antifungal Activity ◽

Active Component ◽

Food Products ◽

Aspergillus Versicolor ◽

Mold Growth ◽

Origanum Vulgare ◽

Emericella Nidulans ◽

Antifungal Activities ◽

Minimum Inhibitory Concentrations

The paper presents the influence of oregano extract (Origanum vulgare L.) on growth of Aspergillus versicolor, Emericella nidulans, Eurotium herbariorum, E. amstelodami, E. chevalieri and E. rubrum - producers of sterigmatocystin (STC) isolated from salads. Antifungal tests were performed by agar plates method. The composition of the active component of extract was determined by GC-MS method and the major components were: carvacrol (34.20%), triacetin (22.91%), carvone (18.05%), p-cymene (8.05%) and thymol (3.74%). The examined extract showed the ability to reduce mold growth at all applied concentrations. Minimum inhibitory concentrations (MIC) for E. nidulans, E. chevalieri and E. amstelodami were 2.5% (v/v) and over 2.5% (v/v) for A. versicolor. At 1.5% (v/v) concentration the extract completely inhibited the growth of E. rubrum, whereas higher dose of 2.5% (v/v) was fungicidal against E. herbariorum. Besides its sensory role in food products, the examined oregano extract also exhibits antifungal activities against producers of STC.

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Biodegradation of poly(butylene succinate-co-butylene adipate) by Aspergillus versicolor

Polymer Degradation and Stability ◽

10.1016/j.polymdegradstab.2005.03.006 ◽

2005 ◽

Vol 90 (1) ◽

pp. 173-179 ◽

Cited By ~ 56

Author(s):

Jian-Hao Zhao ◽

Xiao-Qing Wang ◽

Jun Zeng ◽

Guang Yang ◽

Feng-Hui Shi ◽

...

Keyword(s):

Aspergillus Versicolor ◽

Butylene Succinate

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STORABILITY OF FARM-STORED HULLESS OATS IN MANITOBA

Canadian Journal of Plant Science ◽

10.4141/cjps79-151 ◽

1979 ◽

Vol 59 (4) ◽

pp. 949-957 ◽

Cited By ~ 5

Author(s):

R. N. SINHA ◽

H. A. H. WALLACE ◽

J. T. MILLS ◽

R. I. H. McKENZIE

Keyword(s):

Storage Temperature ◽

First Year ◽

Aspergillus Versicolor ◽

Cryptolestes Ferrugineus ◽

Moisture Contents ◽

Grain Moisture ◽

Avena Nuda ◽

Low Levels ◽

Second Year ◽

Temperature Levels

Storability of properly harvested, mature hulless oats (Avena nuda L. ’Terra’) of 9.7–10.5% moisture content was determined from a 24-mo field trial of two 11.5-t lots of oats stored in two wooden farm bins in Manitoba during 1976–78. Oats were stored safely with very low levels of infestation by preharvest fungi, mites and insects and negligible quality loss for up to about 20 mo. Afterwards the grain moisture contents at the bottom layers of both bins rose from about 10 to 13–16%; this led to heavy floor level infestations by the fungi Penicillium and Aspergillus versicolor, and mites Caloglyphus berlesei and Tarsonemus granarius. Fat acidity values rose from the first year levels of about 30 to 50–78 mg KOH/100 g of dry grain. Populations of Cryptolestes ferrugineus and Tribolium castaneum, introduced to one bin, did poorly during the first summer and failed to survive during the second year. A third beetle, Oryzaephilus surinamensis, continued to multiply in small numbers after 22 mo. Low storage temperature levels during most of the years seemed to have affected adversely the multiplication of insects and to a lesser extent mites in farm-stored oats.

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