Integrative analysis of transcriptome and metabolome reveal mechanism of tolerance to salt stress in oat (Avena sativa L.)

An effort was made to identify the in vitro responsiveness for callus induction in NaCl (salt stress) and subsequent plant regeneration of oats (Avena sativa L.). Callus induction was tested in different concentrations (0, 5, 10, 15 and 20 mg/l) of NaCl salt added in MS. The experiment was laid out in CRD considering 2,4-D growth regulator and NaCl salt as factors. The best response in callus induction was observed on MS containing 3 mg/l 2,4- D and 5 mg/l NaCl where the frequency of callus induction was 68.57%, callus weight was 0.36 g and callus diameter was 7.63 mm. During regeneration, the maximum frequency of regeneration (41.67%), regenerated shoots per callus (14.80), shoot length (12.14 cm), root number (10.90) and root length (3.76 cm) was observed from the callus derived from MS containing 3 mg/l 2,4-D and 5 mg/l NaCl. Plantlets that were regenerated from callus induced in MS with 3 mg/l 2,4-D and 5 mg/l NaCl showed higher rate of ex vitro survivability. Therefore, MS with 3 mg/l 2,4-D and 5 mg/l NaCl found better for in vitro callus induction and subsequent plant regeneration from mature seeds of oat. Plant Tissue Cult. & Biotech. 30(2): 253-266, 2020 (December)

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The beta subunit of glyceraldehyde 3-phosphate dehydrogenase is an important factor for maintaining photosynthesis and plant development under salt stress—Based on an integrative analysis of the structural, physiological and proteomic changes in chloroplasts in Thellungiella halophila

Plant Science ◽

10.1016/j.plantsci.2015.04.010 ◽

2015 ◽

Vol 236 ◽

pp. 223-238 ◽

Cited By ~ 20

Author(s):

Lili Chang ◽

Anping Guo ◽

Xiang Jin ◽

Qian Yang ◽

Dan Wang ◽

...

Keyword(s):

Salt Stress ◽

Plant Development ◽

Integrative Analysis ◽

Beta Subunit ◽

Thellungiella Halophila

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Melatonin Positively Influences the Photosynthetic Machinery and Antioxidant System of Avena sativa during Salinity Stress

Plants ◽

10.3390/plants8120610 ◽

2019 ◽

Vol 8 (12) ◽

pp. 610 ◽

Cited By ~ 4

Author(s):

Nisha Varghese ◽

Onoud Alyammahi ◽

Sarah Nasreddine ◽

Abla Alhassani ◽

Mayank Anand Gururani

Keyword(s):

Salt Stress ◽

Salinity Stress ◽

Avena Sativa ◽

Growth Parameters ◽

Photosynthetic Apparatus ◽

The Other ◽

Photosynthetic Parameters ◽

Transcription Factor Genes ◽

Genes Encoding ◽

Almost All

Recent studies have demonstrated melatonin protects various crops against abiotic stresses. However, the effects of melatonin on the photosynthetic apparatus of stressed plants is poorly characterized. We investigated the effects of melatonin pretreatment on photosynthesis and tolerance to salinity stress in Avena sativa (oat) plants. Oat plants were exposed to four treatments (three replicate pots per treatment): well-watered (WW; control); watered with 300 mM salt solution for 10 days (NaCl); pretreated with 100 µM melatonin solution for 7 days then watered normally for 10 days (Mel+W); or pretreated with 100 µM melatonin for 7 days then 300 mM salt for 10 days (Mel+NaCl). Considerable differences in growth parameters, chlorophyll content, stomatal conductance, proline accumulation, lipid peroxidation, electrolyte leakage, and growth parameters were observed between groups. Genes encoding three major antioxidant enzymes were upregulated in the Mel+NaCl group compared to the other groups. Chlorophyll-a fluorescence kinetic analyses revealed that almost all photosynthetic parameters were improved in Mel+NaCl plants compared to the other treatments. Analysis of genes encoding the major extrinsic proteins of photosystem II (PSII) revealed that PsbA, PsbB, PsbC, and PsbD (but not PsbO) were highly upregulated in Mel+NaCl plants compared to the other groups, indicating melatonin positively influenced photosynthesis under control conditions and salt stress. In addition, melatonin upregulated stress-responsive NAC transcription factor genes in plants exposed to salt stress. These findings suggest melatonin pretreatment improves photosynthesis and enhances salt tolerance in oat plants.

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Integrative analysis of metabolome and transcriptome reveals molecular regulatory mechanism of flavonoid biosynthesis in Cyclocarya paliurus under salt stress

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113823 ◽

2021 ◽

Vol 170 ◽

pp. 113823

Author(s):

Lei Zhang ◽

Zijie Zhang ◽

Shengzuo Fang ◽

Yang Liu ◽

Xulan Shang

Keyword(s):

Salt Stress ◽

Regulatory Mechanism ◽

Flavonoid Biosynthesis ◽

Integrative Analysis ◽

Cyclocarya Paliurus

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SEM Cold Stage Techniques for the Observation of Vegetative and Floral Apices of Oat (Avena sativa L.) Plants

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080377 ◽

1977 ◽

Vol 35 ◽

pp. 590-591

Author(s):

B. K. Kirchoff ◽

L.F. Allard ◽

W.C. Bigelow

Keyword(s):

Critical Point ◽

Avena Sativa ◽

Substantial Improvement ◽

Fresh Tissue ◽

Cold Stage ◽

Critical Point Drying ◽

Almost All ◽

Cell Collapse ◽

Conductive Paint ◽

Carbon Based

In attempting to use the SEM to investigate the transition from the vegetative to the floral state in oat (Avena sativa L.) it was discovered that the procedures of fixation and critical point drying (CPD), and fresh tissue examination of the specimens gave unsatisfactory results. In most cases, by using these techniques, cells of the tissue were collapsed or otherwise visibly distorted. Figure 1 shows the results of fixation with 4.5% formaldehyde-gluteraldehyde followed by CPD. Almost all cellular detail has been obscured by the resulting shrinkage distortions. The larger cracks seen on the left of the picture may be due to dissection damage, rather than CPD. The results of observation of fresh tissue are seen in Fig. 2. Although there is a substantial improvement over CPD, some cell collapse still occurs.Due to these difficulties, it was decided to experiment with cold stage techniques. The specimens to be observed were dissected out and attached to the sample stub using a carbon based conductive paint in acetone.

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