Effect of exogenous silicon and salt stress on germination and seedling establishment in Borago officinalis

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
F Torabi
2013 ◽  
Vol 23 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Rafika Yacoubi ◽  
Claudette Job ◽  
Maya Belghazi ◽  
Wided Chaibi ◽  
Dominique Job

AbstractAlfalfa (Medicago sativa L.) yield is severely compromised by soil salinity, especially at the level of seedling establishment. This question was addressed by proteomics to decipher whether specific changes in protein accumulation correlate with germination performance of alfalfa seeds submitted to a salinity stress as obtained by imbibing seeds in the presence of NaCl. This study used alfalfa seeds submitted to an osmopriming invigoration treatment that proved very efficient in counteracting the negative effect of salinity stress on germination performance. Comparative proteomic analyses disclosed 94 proteins commonly characterizing the response of both the untreated control and osmoprimed seeds to the experimental salinity stress. Remarkably, many of them, representing 84 proteins, showed contrasting accumulation patterns when comparing the untreated control and osmoprimed seeds submitted to the same salt stress. Thus numerous changes observed in the proteome of the untreated control seeds imbibed in the presence of salt, and presumably accounting for the loss in seed vigour associated with salinity stress, can be substantially reversed in osmoprimed seeds undergoing this stress. These data therefore provide a biochemical understanding of the increase in seed vigour generally observed with primed seeds.


2015 ◽  
Vol 48 (3) ◽  
pp. 43-51 ◽  
Author(s):  
O. Younesi ◽  
A. Moradi

Abstract Priming is one of the seed enhancement methods that might be resulted in increasing seed performance (germination and emergence) under stress conditions, such as salinity. Salinity is a major environmental stress which adversely affects germination and seedling establishment in a wide variety of crops. The experiment was arranged as a factorial in completely randomized design (CRD) at Seed Research Laboratory of College of Agriculture, University of Tehran, Iran. The objective of this research was to evaluate the effect of different priming methods on seed germination of two medicinal plants including lemon balm (Melissa officinalis L.) and cumin (Cuminum cyminum L.) under salinity stress. Treatments were combinations of two levels of salinity stress (0 and 10 dsm−1) and five levels of priming (control = non-priming), GA3, manitol, NaCl and distilled water) with three replications. Seeds of lemon balm and cumin were primed for 24 h at 25°C. Results revealed that different growth traits (including germination percentage, germination rate, seedling dry weight, plumule and radical length) significantly (p=0.05) decreased with applying salinity. However, priming of seeds with different materials particularly GA3 was useful for alleviating salt stress effects and improving germination and seedling establishment under salt stress. Under salinity condition, primed seeds possessed more germination and emergence than control. The result of this experiment is consistent with the hypothesis that under salinity stress, priming can prepare a suitable metabolic reaction in seeds and can improve seed germination performance and seedling establishment.


Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 428
Author(s):  
Walid Raafat Nakhla ◽  
Wenqiang Sun ◽  
Kai Fan ◽  
Kang Yang ◽  
Chaopu Zhang ◽  
...  

Rice is highly sensitive to salinity stress during the seedling establishment phase. Salt stress is widely occurring in cultivated areas and severely affects seed germination ability and seedling establishment, which may result in a complete crop failure. The objective of the present study is to identify quantitative trait loci (QTLs) related to salt tolerance of the germination and seedling stages in a rice backcross inbred line (BIL) population that was derived from a backcross of an Africa rice ACC9 as donor and indica cultivar Zhenshan97 (ZS97) as the recurrent parent. Under salt stress, ACC9 exhibited a higher germination percentage, but more repressed seedling growth than ZS97. Using the BIL population, 23 loci for germination parameters were detected at the germination stage and 46 loci were identified for several morphological and physiological parameters at the seedling stage. Among them, nine and 33 loci with the ACC9 alleles increased salt tolerance at the germination and seedling stages, respectively. Moreover, several major QTLs were found to be co-localized in the same or overlapping regions of previously reported genes for salt stress. These major loci will facilitate improving salt-tolerance rice in genome-breeding programs.


Plant Biology ◽  
2019 ◽  
Vol 21 (6) ◽  
pp. 1110-1118 ◽  
Author(s):  
H. A. Silva ◽  
D. F. A. Oliveira ◽  
A. P. Avelino ◽  
C. E. C. Macêdo ◽  
T. Barros‐Galvão ◽  
...  

2019 ◽  
Vol 9 (10) ◽  
pp. 3501-3511 ◽  
Author(s):  
Hongli Yang ◽  
Linbin Deng ◽  
Hongfang Liu ◽  
Shihang Fan ◽  
Wei Hua ◽  
...  

Alternative oxidases (AOXs) are the terminal oxidase in the cyanide-resistant respiration pathway in plant mitochondria, which play an important role in abiotic stress and are proposed as a functional marker for high tolerant breeding. In this study, ten AOX genes (BnaAOXs) were identified, and CysI and CysII of AOX isoforms were highly conserved in rapeseed. Among them, Bna.AOX1b was mainly expressed in the ovule and displayed varying expression between rapeseed cultivars which showed different salt resistance in seed germination. We identified its mitochondrial localization of this gene. To investigate the function of BnaAOX1b in rapeseed, transgenic rapeseed lines with overexpressed BnaAOX1b were created and seed germination and seedling establishment assays were performed under osmotic, salt, and ABA treatment. The results indicated that overexpression of BnaAOX1b significantly improved seed germination under osmotic and salt stress and weakened ABA sensitivity. In addition, post-germination seedling growth was improved under high salt condition, but showed hypersensitivity to ABA. RNA-sequencing analysis indicated that the genes involved in electron transport or energy pathway were induced and a number of gene responses to salt stress and ABA were regulated in Bna.AOX1b overexpressing seeds. Taken together, our results imply that Bna.AOX1b confers tolerance to osmotic and salt stress in terms of seed germination and seedling establishment by regulating stress responsive genes and the response to ABA, and could be utilized as a candidate gene in transgenic breeding.


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