In vivo action of Lactococcus lactis subsp. lactis isolate (R7) with probiotic potential in the stabilization of cancer cells in the colorectal epithelium

The present study focused on the beneficial effects of Lactococcus lactis subsp lactis CNRZ 1427 with possible use as a therapeutic agent against Sal-monella sp.; also we have proposed different therapeutic possibilities of our situation against a pathogen Salmonella sp. We have conducted two tests In Vitro and In Vivo; where it is noted that treatment in the presence of this lac-tic strain is effective since it causes a remarkable decrease of the pathogen agent. At the end, the effectiveness of this lactic strain was confirmed by testing for resistance to gastrointestinal conditions (pH, bile salts and diges-tive enzymes).

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Anti-inflammatory Activities of Lactococcus lactis subsp. cremoris FC in In Vitro and In Vivo Gut Inflammation Models

Bioscience and Microflora ◽

10.12938/bifidus.29.169 ◽

2010 ◽

Vol 29 (4) ◽

pp. 169-178 ◽

Cited By ~ 4

Author(s):

Yosuke Nishitani ◽

Masashi Mizuno

Keyword(s):

Lactococcus Lactis ◽

Gut Inflammation ◽

Lactococcus Lactis Subsp ◽

Anti Inflammatory

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Survival of lactococci during passage through mouse digestive tract

Canadian Journal of Microbiology ◽

10.1139/w03-092 ◽

2003 ◽

Vol 49 (11) ◽

pp. 707-711 ◽

Cited By ~ 58

Author(s):

Hiromi Kimoto ◽

Masaru Nomura ◽

Miho Kobayashi ◽

Koko Mizumachi ◽

Takashi Okamoto

Keyword(s):

Survival Rate ◽

Lactococcus Lactis ◽

Animal Health ◽

In Vitro Test ◽

Lactococcus Lactis Subsp ◽

Mouse Intestine ◽

Vitro Test ◽

The Subject

One of the important properties of probiotics is the ability to survive in the intestine. There have been few studies on the probiotic property of lactococci, since they are formally not considered to be natural inhabitants of the intestine. To evaluate lactococci as probiotic bacteria, we investigated their ability to survive during gastric transit by in vitro and in vivo tests. When exposed to an in vitro simulated gastrointestinal environment, such as low pH and bile, only Lactococcus lactis subsp. lactis bv. diacetylactis N7 showed a moderate survival rate among the four strains tested. The tested strains were orally administered to mice, and intestinal passage of the ingested strains was monitored by two methods: antibiotics and PCR. Viable cells of strain N7 were recovered from feces within 24–48 h after administration but not at 72 h. Lactococcus lactis subsp. cremoris ATCC 19257, which had a poor survival rate in vitro test, was also detected at 12 h but not at 24 h. These results indicate that lactococci can reach the mouse intestine alive, but not colonize it. If administered daily, viable strain N7 may exist continuously in the intestine. The effect of strain N7 on intestinal microbial balance and on animal health will be the subject of a further study.Key words: Lactococcus, survival, gastrointestinal tract, probiotics.

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Evaluation of Some Factors with Significant Influence on Functionality and Viability of Probiotic Strains LA-5 Â® and L. Casei 431 Â® in Multiple Starter Cultures in Mesophilic Fermented Milk

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:8783 ◽

2012 ◽

Vol 69 (2) ◽

Author(s):

Daniela PARASCHIV ◽

Aida VASILE

Keyword(s):

Lactococcus Lactis ◽

Fermented Milk ◽

Starter Cultures ◽

Milk Product ◽

Lactococcus Lactis Subsp ◽

Commercial Code ◽

Long Time ◽

Probiotic Strains

Abstract.Â For a very long time, probiotics have been selected on the basis of their suitability to foodâ€™s environment and technological procedures, as well as survival ability in vitro and also in vivo. These criteria are still used. The aim of this study was to select, using the Plakett-Burman design of experiments model, of three significant factors, which influence the multiplication ability and viability of probiotic strains Lactobacillus acidophilus (commercial code LA-5Â®) and Lactobacillus casei subsp. paracasei (commercial code L. casei 431Â®) in fermented mesophilic milk product (commercial named Sana) obtained by fermentation with a multiple culture with mesophilic lactic starter Flora Danica Chr. Hansen commercial starters (Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. diacetylactis and Leuconostoc mesenteroides subsp. cremoris). By statistical analysis the most important factors (independent variables)Â with influence on the probiotic bacteria were selected for the optimized. These are the temperature of fermentation; the ratio between probiotic strains and Flora Danica and the addition of prebiotic Fagopyrum esculentum flour. In the future experiments the optimum values of these parameters will be optimized by using response surface methodology, in order to establish the conditions in which can be obtain the mesophilic fermented milk products with probiotic action in vivo.Â

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Bacteriocinogenic Lactococcus lactis subsp: lactis DF04Mi isolated from goat milk: Evaluation of the probiotic potential

Brazilian Journal of Microbiology ◽

10.1590/s1517-83822014000300038 ◽

2014 ◽

Vol 45 (3) ◽

pp. 1047-1054 ◽

Cited By ~ 9

Author(s):

Danielle N. Furtado ◽

Svetoslav D. Todorov ◽

Mariza Landgraf ◽

Maria T. Destro ◽

Bernadette D.G.M. Franco

Keyword(s):

Lactococcus Lactis ◽

Goat Milk ◽

Lactococcus Lactis Subsp ◽

Probiotic Potential

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The Anti-Tumor Effect of Lactococcus lactis Bacteria-Secreting Human Soluble TRAIL Can Be Enhanced by Metformin Both In Vitro and In Vivo in a Mouse Model of Human Colorectal Cancer

Cancers ◽

10.3390/cancers13123004 ◽

2021 ◽

Vol 13 (12) ◽

pp. 3004

Author(s):

Katarzyna Kaczmarek ◽

Jerzy Więckiewicz ◽

Kazimierz Węglarczyk ◽

Maciej Siedlar ◽

Jarek Baran

Keyword(s):

Mouse Model ◽

Cancer Cells ◽

Lactococcus Lactis ◽

Expression System ◽

Local Delivery ◽

Biologically Active ◽

Soluble Trail ◽

Anti Tumor Activity

Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) induces apoptosis of many cancer cells, including CRC cells, being non-harmful for normal ones. However, recombinant form of human TRAIL failed in clinical trial when administered intravenously. To assess the importance of TRAIL in CRC patients, new form of TRAIL delivery would be required. Here we used genetically modified, non-pathogenic Lactococcus lactis bacteria as a vehicle for local delivery of human soluble TRAIL (hsTRAIL) in CRC. Operating under the Nisin Controlled Gene Expression System (NICE), the modified bacteria (L. lactis(hsTRAIL+)) were able to induce cell death of HCT116 and SW480 human cancer cells and reduce the growth of HCT116-tumor spheres in vitro. This effect was cancer cell specific as the cells of normal colon epithelium (FHC cells) were not affected by hsTRAIL-producing bacteria. Metformin (MetF), 5-fluorouracil (5-FU) and irinotecan (CPT-11) enhanced the anti-tumor actions of hsTRAIL in vitro. In the NOD-SCID mouse model, treatment of subcutaneous HCT116-tumors with L. lactis(hsTRAIL+) bacteria given intratumorally, significantly reduced the tumor growth. This anti-tumor activity of hsTRAIL in vivo was further enhanced by oral administration of MetF. These findings indicate that L. lactis bacteria could be suitable for local delivery of biologically active human proteins. At the same time, we documented that anti-tumor activity of hsTRAIL in experimental therapy of CRC can be further enhanced by MetF given orally, opening a venue for alternative CRC-treatment strategies.

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Ultrastructural modification of cellular interactions in cancer tumor

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082455 ◽

1978 ◽

Vol 36 (2) ◽

pp. 318-319

Author(s):

N. P. Dmitrieva

Keyword(s):

Cancer Cells ◽

Human Thyroid ◽

Adjacent Cell ◽

Main Role ◽

Cellular Interactions ◽

Electron Microscopic ◽

Cancer Tumor ◽

Normal Human ◽

Characteristic Features

One of the most characteristic features of cancer cells is their ability to metastasia. It is suggested that the modifications of the structure and properties of cancer cells surfaces play the main role in this process. The present work was aimed at finding out what ultrastructural features apear in tumor in vivo which removal of individual cancer cells from the cell population can provide. For this purpose the cellular interactions in the normal human thyroid and cancer tumor of this gland electron microscopic were studied. The tissues were fixed in osmium tetroxide and were embedded in Araldite-Epon.In normal human thyroid the most common type of intercellular contacts was represented by simple junction formed by the parallelalignment of adjacent cell membranees leaving in between an intermembranes space 15-20 nm filled with electronlucid material (Fig. 1a). Sometimes in the basal part of cells dilatations of the intercellular space 40-50 nm wide were found (Fig. 1a). Here the cell surfaces may form single short microvilli.

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