scholarly journals Evaluation of Some Factors with Significant Influence on Functionality and Viability of Probiotic Strains LA-5 ® and L. Casei 431 ® in Multiple Starter Cultures in Mesophilic Fermented Milk

Author(s):  
Daniela PARASCHIV ◽  
Aida VASILE

Abstract.  For a very long time, probiotics have been selected on the basis of their suitability to food’s environment and technological procedures, as well as survival ability in vitro and also in vivo. These criteria are still used. The aim of this study was to select, using the Plakett-Burman design of experiments model, of three significant factors, which influence the multiplication ability and viability of probiotic strains Lactobacillus acidophilus (commercial code LA-5®) and Lactobacillus casei subsp. paracasei (commercial code L. casei 431®) in fermented mesophilic milk product (commercial named Sana) obtained by fermentation with a multiple culture with mesophilic lactic starter Flora Danica Chr. Hansen commercial starters (Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. diacetylactis and Leuconostoc mesenteroides subsp. cremoris). By statistical analysis the most important factors (independent variables)  with influence on the probiotic bacteria were selected for the optimized. These are the temperature of fermentation; the ratio between probiotic strains and Flora Danica and the addition of prebiotic Fagopyrum esculentum flour. In the future experiments the optimum values of these parameters will be optimized by using response surface methodology, in order to establish the conditions in which can be obtain the mesophilic fermented milk products with probiotic action in vivo. 

2021 ◽  
pp. 23-26
Author(s):  
Татьяна Алексеевна Антипова ◽  
Светлана Валерьевна Фелик ◽  
Надежда Леонидовна Андросова ◽  
Сергей Владимирович Симоненко

Статья посвящена получению кисломолочного продукта для питания детей с лактазной недостаточностью. Одним из наиболее распространенных способов получения низколактозных продуктов является применение гидролиза лактозы с использованием ферментных препаратов. Целью работы было изучение процесса сквашивания низколактозного молочного продукта. Учитывая, что при использовании методов ферментирования лактозы продукт приобретает выраженный сладкий вкус, обусловленный наличием глюкозы, требовалось установить заданное количество лактозы при достижении оптимальных органолептических показателей. Результаты исследований свидетельствуют об изменении органолептических и физико-химических показателей образцов в результате гидролиза лактозы. Вкусовые проявления сладости в продукте появляются при степени гидролиза лактозы 50 % и усиливаются при дальнейшем проведении гидролиза. Для проведения процесса сквашивания применяли следующие виды заквасочных культур: Lactobacillus acidophilus, Treptococcus thermophilus, Lactobacillus bulgaricus, Streptococcus thermophiles, Lactococcus lactis subsp. lactis и Lactococcus lactis subsp. Сremoris. Процесс сквашивания во всех образцах низколактозного молока имеет свои особенности, характерные для используемой заквасочной культуры. Следует отметить, что заквасочные культуры, включающие ацидофильную палочку, интенсифицируют процесс сквашивания исследуемых образцов. Содержание лактозы после окончания процесса сквашивания в исследуемых образцах составило 1,35; 1,4 %, что соответствует требованиям ТР ТС 027/2012 «О безопасности отдельных видов специализированной пищевой продукции, в том числе диетического лечебного и диетического профилактического питания». The article is devoted to the preparation of a fermented milk product for the nutrition of children with lactase deficiency. One of the most common methods of obtaining low-lactose products is the use of lactose hydrolysis using enzyme preparations. The aim of the work was to study the process of fermentation of low-lactose dairy product. Given that when using the methods of lactose fermentation, the product acquires a pronounced sweet taste due to the presence of glucose, it was necessary to set a given amount of lactose when achieving optimal organoleptic parameters. The results of the studies indicate a change in the organoleptic and physico-chemical parameters of the samples as a result of lactose hydrolysis. The taste manifestations of sweetness in the product appear at the degree of lactose hydrolysis of 50 % and increase with further hydrolysis. The following types of starter cultures were used for the fermentation process: Lactobacillus acidophilus; Treptococcus thermophilus, Lactobacillus bulgaricus; Streptococcus thermophiles; Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. Cremoris. The fermentation process in all samples of low-lactose milk has its own characteristics characteristic of the starter culture used. It should be noted that starter cultures, including Acidophilus bacillus, intensify the process of fermentation of the studied samples. The lactose content after the end of the fermentation process in the studied samples was 1.35; 1.4 %, which corresponds to the requirements of TR CU 027/2012 «On the safety of certain types of specialized food products, including dietary therapeutic and dietary preventive nutrition».


2015 ◽  
Vol 4 (5) ◽  
pp. 240-246
Author(s):  
Menad Najett ◽  
Chougrani Fadelaa ◽  
Moghtet Snoussi ◽  
Cheriguene Abderrahim

  The present study focused on the beneficial effects of Lactococcus lactis subsp lactis CNRZ 1427 with possible use as a therapeutic agent against Sal-monella sp.; also we have proposed different therapeutic possibilities of our situation against a pathogen Salmonella sp. We have conducted two tests In Vitro and In Vivo; where it is noted that treatment in the presence of this lac-tic strain is effective since it causes a remarkable decrease of the pathogen agent. At the end, the effectiveness of this lactic strain was confirmed by testing for resistance to gastrointestinal conditions (pH, bile salts and diges-tive enzymes).


2003 ◽  
Vol 49 (11) ◽  
pp. 707-711 ◽  
Author(s):  
Hiromi Kimoto ◽  
Masaru Nomura ◽  
Miho Kobayashi ◽  
Koko Mizumachi ◽  
Takashi Okamoto

One of the important properties of probiotics is the ability to survive in the intestine. There have been few studies on the probiotic property of lactococci, since they are formally not considered to be natural inhabitants of the intestine. To evaluate lactococci as probiotic bacteria, we investigated their ability to survive during gastric transit by in vitro and in vivo tests. When exposed to an in vitro simulated gastrointestinal environment, such as low pH and bile, only Lactococcus lactis subsp. lactis bv. diacetylactis N7 showed a moderate survival rate among the four strains tested. The tested strains were orally administered to mice, and intestinal passage of the ingested strains was monitored by two methods: antibiotics and PCR. Viable cells of strain N7 were recovered from feces within 24–48 h after administration but not at 72 h. Lactococcus lactis subsp. cremoris ATCC 19257, which had a poor survival rate in vitro test, was also detected at 12 h but not at 24 h. These results indicate that lactococci can reach the mouse intestine alive, but not colonize it. If administered daily, viable strain N7 may exist continuously in the intestine. The effect of strain N7 on intestinal microbial balance and on animal health will be the subject of a further study.Key words: Lactococcus, survival, gastrointestinal tract, probiotics.


2021 ◽  
Vol 9 (2) ◽  
pp. 349
Author(s):  
Natalia Garcia-Gonzalez ◽  
Natalia Battista ◽  
Roberta Prete ◽  
Aldo Corsetti

Fermentation processes have been used for centuries for food production and preservation. Besides the contribution of fermentation to food quality, recently, scientific interest in the beneficial nature of fermented foods as a reservoir of probiotic candidates is increasing. Fermented food microbes are gaining attention for their health-promoting potential and for being genetically related to human probiotic bacteria. Among them, Lactiplantibacillus (Lpb.) plantarum strains, with a long history in the food industry as starter cultures in the production of a wide variety of fermented foods, are being investigated for their beneficial properties which are similar to those of probiotic strains, and they are also applied in clinical interventions. Food-associated Lpb. plantarum showed a good adaptation and adhesion ability in the gastro-intestinal tract and the potential to affect host health through various beneficial activities, e.g., antimicrobial, antioxidative, antigenotoxic, anti-inflammatory and immunomodulatory, in several in vitro and in vivo studies. This review provides an overview of fermented-associated Lpb. plantarum health benefits with evidence from clinical studies. Probiotic criteria that fermented-associated microbes need to fulfil are also reported.


2020 ◽  
pp. 28-39
Author(s):  
Oniovosa Leonard Adamu- Governor ◽  
Peter Nwabueze Okolie ◽  
Emmanuel Mmmaduabuchi Ikegwu ◽  
Ifedinma Henrietta Obeten ◽  
Christiana Oluwatoyin Abiona ◽  
...  

Yoghurt remains a fermented milk of choice globally but its desirability is limited by quality attributes and syneresis. In this study, the effect of using exopolysaccharide (EPS) producing starter cultures and EPS on the quality attribute of yoghurt produced from cow milk was examined. Two starter cultures of EPS-producing LAB were used singly and in combination in three treatments portions (YEPSLa, Lactobacillus acidophilus yoghurt; YEPSLs, Leuconostoc suionicum; YEPSLa + YEPSLs, Lactobacillus acidophilus and Leuconostoc suionicum); Yxg, Streptococcus thermophilus and Lactobacillus delbrueckii subsp. Bulgaricus to produced yoghurt while reference yoghurt (RY) was obtained from the market and refrigerated stored at 4oC. Sensory, proximate composition, textural, rheological properties and whey separation were carried out after 1 and 28 days only, while physicochemical and microbiological were analyzed after 1, 7, 14 and 28 days. No significant difference (p ˂0.05) between RY (8.60 ± 0.60), (7.21±0.10) and YEPSLa + YEPSLs (8.54 ± 0.71), (7.25 ± 0.21) in overall acceptability for day 1 and 28. Moisture (82.45 ± 0.12 - 81.31 ± 0.06%), fat (3.46 ± 0.01 - 3.42 ± 0.03%) and carbohydrate (13.05 ± 0.11 to 12.51 ± 0.10%) contents decreased while total solids (17.57 ± 0.12 - 17.97 ± 0.12%), ash (0.56 ± 0.02 - 0.57 ± 0.02%) and protein (3.74 ± 0.01 - 4.30%) contents increased respectively across the yoghurts. The result showed that the highest cohesiveness and syneresis was observed in YEPSLa + YEPSLs (27.52 ± 0.63) and commercial yoghurt (29.10 ± 0.31), the lowest in Yxg (16.71 ± 0.21) and YEPSLa + YEPSLs (21.50 ± 0.51). The highest viscosity was observed in YEPSLa + YEPSLs across the rotation speeds.  The pH and titratable acid ranged (4.28 – 4.50; 0.90 – 1.41) while the total bacteria colony count (5.5×108 – 11.0×108cfu/ml) during 28 days storage period. Overall, EPS produced by EPS-producing LAB both In-vitro and In-vivo improve texture, mouthfeel, viscosity and reduce syneresis in yoghurt. Combine cultures of Lactobacillus acidophilus and Leuconostoc suionicum and their EPSs competed favourably with conventional starter, and other stabilizing agents in cow milk yoghurt.


2018 ◽  
Vol 18 (6) ◽  
pp. 769-775 ◽  
Author(s):  
Dayun Yan ◽  
Jonathan H. Sherman ◽  
Michael Keidar

Background: Over the past five years, the cold atmospheric plasma-activated solutions (PAS) have shown their promissing application in cancer treatment. Similar as the common direct cold plasma treatment, PAS shows a selective anti-cancer capacity in vitro and in vivo. However, different from the direct cold atmospheric plasma (CAP) treatment, PAS can be stored for a long time and can be used without dependence on a CAP device. The research on PAS is gradually becoming a hot topic in plasma medicine. Objectives: In this review, we gave a concise but comprehensive summary on key topics about PAS including the development, current status, as well as the main conclusions about the anti-cancer mechanism achieved in past years. The approaches to make strong and stable PAS are also summarized.


Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1451
Author(s):  
Carolina Romeiro Fernandes Chagas ◽  
Josef Harl ◽  
Vytautas Preikša ◽  
Dovilė Bukauskaitė ◽  
Mikas Ilgūnas ◽  
...  

Recent studies confirmed that some Hepatozoon-like blood parasites (Apicomplexa) of birds are closely related to the amphibian parasite Lankesterella minima. Little is known about the biology of these pathogens in birds, including their distribution, life cycles, specificity, vectors, and molecular characterization. Using blood samples of 641 birds from 16 species, we (i) determined the prevalence and molecular diversity of Lankesterella parasites in naturally infected birds; (ii) investigated the development of Lankesterella kabeeni in laboratory-reared mosquitoes, Culex pipiens forma molestus and Aedes aegypti; and (iii) tested experimentally the susceptibility of domestic canaries, Serinus canaria, to this parasite. This study combined molecular and morphological diagnostic methods and determined 11% prevalence of Lankesterella parasites in Acrocephalidae birds; 16 Lankesterella lineages with a certain degree of host specificity and two new species (Lankesterella vacuolata n. sp. and Lankesterella macrovacuolata n. sp.) were found and characterized. Lankesterella kabeeni (formerly Hepatozoon kabeeni) was re-described. Serinus canaria were resistant after various experimental exposures. Lankesterella sporozoites rapidly escaped from host cells in vitro. Sporozoites persisted for a long time in infected mosquitoes (up to 42 days post exposure). Our study demonstrated a high diversity of Lankesterella parasites in birds, and showed that several avian Hepatozoon-like parasites, in fact, belong to Lankesterella genus.


Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1428
Author(s):  
Ramachandran Chelliah ◽  
Eun-Ji Kim ◽  
Eric Banan-Mwine Daliri ◽  
Usha Antony ◽  
Deog-Hwan Oh

In the present study, we screened for potential probiotic yeast that could survive under extreme frozen conditions. The antimicrobial and heat-stable properties of the isolated yeast strains Saccharomyces boulardii (S. boulardii) (KT000032, KT000033, KT000034, KT000035, KT000036, and KT000037) was analyzed and compared with commercial probiotic strains. The results revealed that the tested S. boulardii KT000032 strain showed higher resistance to gastric enzymes (bile salts, pepsin, and pancreatic enzyme) at low pH, with broad antibiotic resistance. In addition, the strain also showed efficient auto-aggregation and co-aggregation abilities and efficient hydrophobicity in the in-vitro and in-vivo C. elegens gut model. Further, the KT000032 strain showed higher antimicrobial efficiency against 13 different enteropathogens and exhibited commensal relationships with five commercial probiotic strains. Besides, the bioactive compounds produced in the cell-free supernatant of probiotic yeast showed thermo-tolerance (95 °C for two hours). Furthermore, the thermo-stable property of the strains will facilitate their incorporation into ready-to-eat food products under extreme food processing conditions.


2018 ◽  
Vol 7 (19) ◽  
Author(s):  
Thao D. Tran ◽  
Steven Huynh ◽  
Craig T. Parker ◽  
Ruyang Han ◽  
Robert Hnasko ◽  
...  

We present here the complete genome sequence of Lactococcus lactis strain 14B4, isolated from almond drupes in northern California. This strain was observed to inhibit the growth of Salmonella enterica serotype Poona strain RM3363 in vitro.


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