scholarly journals A Simple and Reliable Semipreparative High-Performance Liquid Chromatography Technique for the Isolation of Marker-Grade Hyperforin from Hypericum perforatum L. Extract

2003 ◽  
Vol 41 (8) ◽  
pp. 444-444 ◽  
Author(s):  
R. Anand ◽  
N. Verma ◽  
G. Handa ◽  
R. K. Khajuria ◽  
V. K. Gupta ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Hypericum Perforatum L

scholarly journals Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

2010 ◽  
Vol 8 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Abdulkadir Levent ◽  
Suat Ekin ◽  
Gökhan Oto
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Treated Group ◽  
Retinyl Palmitate ◽  
Rat Serum ◽  
Array Detection ◽  
Hypericum Perforatum L ◽  
Β Carotene

AbstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (pa] anthracene + Hypericum Perforatum L. treated group compared to the control group..

scholarly journals THE RESEARCH OF THE SPECTRUM OF BIOLOGICALLY ACTIVE FLAVONOIDS GRASS HYPERICUM PERFORA-TUM L. FLORA OF THE REPUBLIC OF TATARSTAN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2017 ◽  
pp. 175-179
Author(s):  
Ландыш (Landysh) Завдетовна (Zavdetovna) Хуснетдинова (Khusnetdinova) ◽  
Антон (Anton) Николаевич (Nikolaevich) Акулов (Akulov) ◽  
Светлана (Svetlana) Алексеевна (Alekseevna) Дубровная (Dubrovnaya)
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Raw Materials ◽  
Growth Conditions ◽  
Climatic Conditions ◽  
Biologically Active ◽  
Plant Raw Materials ◽  
The Republic

The article presents the results of the study of the dependence of the flavonoid content of the herb Hypericum perforatum L., from growth conditions of cenopopulations in various ecological, cenotic conditions of the Republic of Tatarstan. Plant biocenosis were considered – meadows, Border of broadleaf and pine forest or artificial planting of pine. A qualitative and quantitative analysis of flavonoids in the herb of  Hypericum perforatum was carried out by high-performance liquid chromatography. In studying water-alcohol extracts of H. perforatum L.  flavonoids – rutin, quercetin, bisapigenin were identified by HPLC analysis. It is shown that the ecological, cenotic conditions of growth of Hypericum perforatum affect the quantitative content of phenolic compounds. It has been established that in investigated cenopopulations in the territory of the Republic of Tatarstan, rutin is the dominant flavonoid in medicinal plant raw materials. Maximum amount of this compound detected in the samples of Hypericum perforatum confined to the pine  biocenosis of Tukaevsky and Drozhzhanovsky districts under significant quantity level of quercetin. In all variants of the experiment, bisapiginin was detected in small amounts. Plants of Hypericum perforatum, grow in different plant  biocenosis differed in the various contents of the compounds of the phenolic complex, which is probably due to the ecological, cenotic and climatic conditions of growth. 

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