Tiotropium bromide inhibits relapsing allergic asthma in BALB/c mice

2014 ◽  
Vol 27 (1) ◽  
pp. 44-51 ◽  
Author(s):  
Berislav Bosnjak ◽  
Cornelia Tilp ◽  
Christopher Tomsic ◽  
Gerhard Dekan ◽  
Michael P. Pieper ◽  
...  
2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Juan Wang ◽  
Xiaolin Diao ◽  
Hong Zhu ◽  
Bei He

Rationale. We previously demonstrated increased expression of programmed cell death 5 (PDCD5) in asthmatic patients and ovalbumin-induced allergic asthma. International guidelines (GINA 2019) have included the use of tiotropium bromide for chronic treatment of the most severe and frequently exacerbated asthma in patients ≥6 years old, who do not have good response to inhaled corticosteroids. Objective. To explore the role of tiotropium and its effect on PDCD5 level in a mouse model of chronic asthma. Methods. We divided 12 female mice into 2 groups: untreated asthma (n = 6) and tiotropium-treated asthma (n = 6). The impact of tiotropium was assessed by histology of lung tissue and morphometry. Pulmonary function was tested by using pressure sensors. The number of cells in bronchoalveolar lavage fluid (BALF) was detected. Levels of PDCD5, active caspase-3, and muscarinic acetylcholine receptors M2 (ChRM2) and M3 (ChRM3) were examined. Results. Tiotropium treatment significantly reduced airway inflammation and remodeling in asthmatic mice and intensified the lung function. PDCD5 level was reduced with tiotropium (p<0.05). Moreover, active caspase-3 level was decreased with tiotropium (p<0.001), and ChRM3 level was increased. Conclusions. Tiotropium treatment may alleviate the pathological changes with asthma by regulating apoptosis.


Author(s):  
Kate W. Sjoerdsma ◽  
W. James Metzger

Eosinophils are important to the pathogenesis of allergic asthma, and are increased in bronchoalveolar lavage within four hours after bronchoprovocation of allergic asthmatic patients, and remain significantly increased up to 24 hours later. While the components of human eosinophil granules have been recently isolated and purified, the mechanisms of degranulation have yet to be elucidated.We obtained blood from two volunteers who had a history of allergic rhinitis and asthma and a positive skin test (5x5mm wheal) to Alternaria and Ragweed. Eosinophils were obtained using a modification of the method described by Roberts and Gallin.


2001 ◽  
Vol 58 (5) ◽  
pp. 315-320
Author(s):  
C. Bucher ◽  
E. W. J. Russi

Asthma bronchiale ist eine chronische entzündliche Erkrankung der Atemwege, die gehäuft bei Atopikern auftritt. Die Therapie des allergischen Asthma bronchiale stützt sich auf drei Pfeiler: Das Vermeiden einer Allergenexposition, eine medikamentöse Therapie sowie in ausgewählten Fällen eine spezifische Immuntherapie (SIT). Ein völliges Meiden des Allergenkontaktes ist in der Regel nicht zu erreichen, weshalb die meisten Asthmatiker eine medikamentöse Behandlung benötigen. Dafür stehen moderne und wirksame Medikamente zur Verfügung. Die SIT hat sich vor allem bei der Pollenallergie bewährt. Wegen des in der Regel chronischen und wechselhaften Verlaufs sowie mitunter lebensbedrohlichen Situationen sollte der Patient über die Natur der Erkrankung, die Therapie, notwendige Kontrollen, sowie Maßnahmen bei einer plötzlichen Verschlechterung sorgfältig instruiert werden. Allgemein gilt für die Therapie des Asthma bronchiale, dass die Compliance des Patienten für den Therapieerfolg entscheidend ist.


Pneumologie ◽  
2011 ◽  
Vol 65 (12) ◽  
Author(s):  
L Lunding ◽  
C Vock ◽  
H Fehrenbach ◽  
M Wegmann
Keyword(s):  

Pneumologie ◽  
2012 ◽  
Vol 66 (06) ◽  
Author(s):  
A Turowska ◽  
T Dicke ◽  
N Baumgartl ◽  
J Kuhlmann ◽  
H Renz ◽  
...  

Pneumologie ◽  
2012 ◽  
Vol 66 (06) ◽  
Author(s):  
R Nandigama ◽  
A Weske ◽  
S Wiegand ◽  
W Kummer ◽  
C Nassenstein
Keyword(s):  

Pneumologie ◽  
2012 ◽  
Vol 66 (06) ◽  
Author(s):  
R Boehmer ◽  
R Nandigama ◽  
S Wiegand ◽  
J Lee ◽  
W Kummer ◽  
...  
Keyword(s):  

Pneumologie ◽  
2012 ◽  
Vol 66 (11) ◽  
Author(s):  
R Hendriks ◽  
A KleinJan ◽  
M De Bruijn ◽  
M Van Nimwegen ◽  
I Bergen ◽  
...  

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