Effect of gelatin–ionic liquid functional polymers on glucose oxidase and horseradish peroxidase kinetics

AbstractNanohybrid artificial membranes made by intercalation of amphiphilic molecules into the galleries of a layered host, exhibit characteristics similar to biological membranes and they can be used as sensors. Different responses have been observed even for molecules that have similar features for example, saccharin and its sodium salt suggesting that the nanohybrid might be useful in developing an electronic nose. The dynamic range of the saccharin sensor is 20 - 300μM. In this paper we present our results on sensor fabrication and testing and discuss possible sensing mechanisms. In addition, we describe our work on immobilizing in the nanohybrid membranes Glucose Oxidase, Horseradish Peroxidase and Gramicidin and evaluating their performance.

Download Full-text

Glucose Biosensor Based on Glucose Oxidase-Horseradish Peroxidase/Multiporous Tin Oxide (SnO2) Modified Electrode

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2021.19283 ◽

2021 ◽

Vol 21 (5) ◽

pp. 3059-3064

Author(s):

A. K. M. Kafi ◽

Aizam Bin Kasri ◽

Rajan Jose

Keyword(s):

Horseradish Peroxidase ◽

Glucose Oxidase ◽

Tin Oxide ◽

Electrode Potential ◽

High Sensitivity ◽

Glucose Biosensor ◽

Glucose Detection ◽

Lower Detection Limit ◽

Vis Spectroscopy ◽

Lower Detection

The fabrication of a network of glucose oxidase-horseradish peroxidase/tin oxide (GOx-HRP/SnO2), immobilized onto a glassy carbon electrode (GCE) and its utilization as a biosensor for glucose detection is reported. The network established with GOx-HRP/SnO2 possess high sensitivity and stability by performing the electrocatalytic features in the sensing of glucose. The turbidity of fabrication had been scanned and analyzed using UV-vis spectroscopy. The morphology and composition of the fabricated GOx-HRP/SnO2 networks were characterized by scanning electron microscopy (SEM). Cyclic voltammetry and amperometry were employed to study the electrochemical properties of the proposed biosensor. The effect of applied electrode potential and pH were systemically investigated. The biosensor responds to glucose at work potential values between −400 mV, and exhibited a lower detection limit (0.025 mM) and long linear range (0.25 to 7.0 mM), and was resistant to common interferences.

Download Full-text

Corrections

Clinical Chemistry ◽

10.1093/clinchem/19.9.1084 ◽

1973 ◽

Vol 19 (9) ◽

pp. 1084-1084

Keyword(s):

Aspergillus Niger ◽

Horseradish Peroxidase ◽

Glucose Oxidase ◽

Gluconic Acid ◽

Type Ii ◽

Sigma Chemical

Abstract p 1424, Table 2, "units/liter" should read "milliunits/ liter." In reference to the article by Meites, S., and Saniel-Banrey, K., Clin. Chem. 19, 308 (1973), the authors wish to make the following clarification: The glucose oxidase used was purified from Aspergillus niger, and obtained from Sigma Chemical Co., St. Louis, Mo. 63178. It contained 1.5 EU/mg. One unit (EU) will oxidize 1 µmol of glucose to gluconic acid and H2O2 per minute at pH 5.1 at 35 °C. The peroxidase used was horseradish peroxidase and was obtained from Sigma Chemical Co. as Type II (RZ 1.0-1.5), approximately 100-150 Purpurogallin (20-s) Units/mg, or as Worthington "Peroxidase D," 626 U/mg (RZ > 1), Worthington Biochemical Corp., Freehold, N.J. 07728.

Download Full-text

Dynamic monitoring of a bi-enzymatic reaction at a single biomimetic giant vesicle

The Analyst ◽

10.1039/d0an01273d ◽

2020 ◽

Vol 145 (24) ◽

pp. 7922-7931

Author(s):

Pauline Lefrançois ◽

Bertrand Goudeau ◽

Stéphane Arbault

Keyword(s):

Confocal Microscopy ◽

Horseradish Peroxidase ◽

Glucose Oxidase ◽

Enzymatic Reaction ◽

Dynamic Monitoring ◽

Giant Unilamellar Vesicles ◽

Unilamellar Vesicles ◽

Giant Vesicle ◽

Micro Reactors

Giant unilamellar vesicles were used as individual biomimetic micro-reactors wherein a model bi-enzymatic reaction involving a glucose oxidase (GOx) and horseradish peroxidase (HRP) was monitored by confocal microscopy.

Download Full-text