Erratum to “Urotensin II is an autocrine/paracrine growth factor for aortic adventitia of rat” [Regulatory Peptides 151 (2008) 88–94]

2009 ◽  
Vol 154 (1-3) ◽  
pp. 121
Author(s):  
Yonggang Zhang ◽  
Yuguang Li ◽  
Ruihong Wei ◽  
Zhijian Wang ◽  
Dingfang Bu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Regulatory Peptides ◽  
Urotensin Ii ◽  
Paracrine Growth Factor

scholarly journals Urotensin II is an Autocrine/Paracrine Growth Factor for the Porcine Renal Epithelial Cell Line, LLCPK1

Endocrinology ◽  
2003 ◽  
Vol 144 (5) ◽  
pp. 1825-1831 ◽  
Author(s):  
Mika Matsushita ◽  
Masayoshi Shichiri ◽  
Nozomi Fukai ◽  
Naoko Ozawa ◽  
Takanobu Yoshimoto ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Growth Factor ◽  
Kinase Inhibitor ◽  
Epithelial Cell Line ◽  
Urotensin Ii ◽  
Renal Epithelial Cell ◽  
Renal Epithelial Cells ◽  
Kinase C ◽  
Renal Epithelial Cell Line ◽  
Paracrine Growth Factor

Urotensin-II (UII), a cyclic dodecapeptide with potent cardiovascular effects, has recently been shown to be abundantly expressed in the human kidney and excreted in human urine. To investigate whether UII acts as an autocrine/paracrine growth factor for renal epithelial cells, we have studied the effects of human UII (hUII) on DNA synthesis, cytosolic free Ca2+ concentration ([Ca2+]i), ERK activation, and protooncogene (c-myc) expression in a porcine renal epithelial cell line (LLCPK1). hUII stimulated [3H]thymidine uptake into quiescent cells in a dose-dependent manner (10−9 to 10−7m); this effect was inhibited by a protein kinase C inhibitor (GF109203X), a MAPK kinase inhibitor (PD98059), and a calcium channel blocker (nicardipine). Neither phosphatidyl inositol-3 kinase inhibitors (LY294002, wortmannin) nor p38 kinase inhibitor (SB203580) affected the hUII-induced DNA syntheses. hUII rapidly (within 5 min) and dose-dependently (10−9 to 10−7m) increased [Ca2+]i in fura-2-loaded cells. hUII also caused a rapid and transient activation of ERK1/2 and induction of c-myc. LLCPK1 cells expressed UII mRNA and its receptor GPR14 mRNA, as determined by RT-PCR, and released UII-like immunoreactivity into media. Neutralization of endogenous UII by anti-hUII antibody, but not nonimmune serum, significantly suppressed DNA synthesis. These data suggest that hUII is an autocrine/paracrine growth factor for renal epithelial cells via activation of both protein kinase C and ERK1/2 pathways as well as Ca2+ influx via voltage-dependent Ca2+ channels.

Urotensin II is an autocrine/paracrine growth factor for aortic adventitia of rat

2008 ◽  
Vol 151 (1-3) ◽  
pp. 88-94 ◽  
Author(s):  
Yonggang Zhang ◽  
Yuguang Li ◽  
Ruihong Wei ◽  
Zhijian Wang ◽  
Dingfang Bu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Urotensin Ii ◽  
Paracrine Growth Factor

Role of urotensin II in peripheral tissue as an autocrine/paracrine growth factor

Peptides ◽  
2004 ◽  
Vol 25 (10) ◽  
pp. 1775-1781 ◽  
Author(s):  
Takanobu Yoshimoto ◽  
Mika Matsushita ◽  
Yukio Hirata
Keyword(s):  
Growth Factor ◽  
Peripheral Tissue ◽  
Urotensin Ii ◽  
Paracrine Growth Factor

scholarly journals Phorbol ester induces the rapid processing of cell surface heparin-binding EGF-like growth factor: conversion from juxtacrine to paracrine growth factor activity.

1995 ◽  
Vol 6 (8) ◽  
pp. 967-980 ◽  
Author(s):  
K Goishi ◽  
S Higashiyama ◽  
M Klagsbrun ◽  
N Nakano ◽  
T Umata ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Surface ◽  
Vero Cell ◽  
Half Life ◽  
Immunofluorescent Staining ◽  
Simultaneous Increase ◽  
Cell Contact ◽  
Heparin Binding ◽  
Paracrine Growth Factor ◽  
Rapid Processing

Vero cell heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a 20- to 30-kDa membrane-anchored HB-EGF precursor (proHB-EGF). Localization and processing of proHB-EGF, both constitutive and 12-O-tetradecanoylphorbol 13-acetate (TPA)-inducible, was examined in Vero cells overexpressing recombinant HB-EGF (Vero H cells). Flow cytometry and fluorescence immunostaining demonstrated that Vero cell proHB-EGF is cell surface-associated and localized at the interface of cell to cell contact. Cell surface biotinylation and immunoprecipitation detected a 20- to 30-kDa heterogeneous proHB-EGF species. Vero H cell surface proHB-EGF turned over constitutively with a half-life of 1.5 h. Some of the 20- to 30-kDa cell surface-associated proHB-EGF was processed and a 14-kDa species of bioactive HB-EGF was released slowly, but most of the proHB-EGF was internalized, displaying a diffuse immunofluorescent staining pattern and accumulation of proHB-EGF in endosomes. Addition of TPA induced a rapid processing of proHB-EGF at a Pro148-Val149 site with a half-life of 7min. The TPA effect was abrogated by the protein kinase C inhibitors, staurosporine and H7. Kinetic analysis showed that loss of cell surface proHB-EGF is maximal at 30 min after addition of TPA and that proHB-EGF is resynthesized and the initial cell surface levels are regained within 12-24 h. Loss of cell surface proHB-EGF was concomitant with appearance of 14- and 19-kDa soluble HB-EGF species in conditioned medium. Vero H cell-associated proHB-EGF is a juxtacrine growth factor for EP170.7 cells in coculture. Processing of proHB-EGF resulted in loss of juxtacrine activity and a simultaneous increase in soluble HB-EGF paracrine mitogenic activity. It was concluded that processing regulates HB-EGF bioactivity by converting it from a cell-surface juxtacrine growth factor to a processed, released soluble paracrine growth factor.

Mesangial cells express PDGF mRNAs and proliferate in response to PDGF

1988 ◽  
Vol 255 (4) ◽  
pp. F674-F684 ◽  
Author(s):  
P. J. Shultz ◽  
P. E. DiCorleto ◽  
B. J. Silver ◽  
H. E. Abboud
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Northern Blot Analysis ◽  
Mesangial Cells ◽  
Glomerular Mesangial Cells ◽  
Human Mesangial Cells ◽  
Human Foreskin Fibroblasts ◽  
Human Glomerular Mesangial Cells ◽  
Paracrine Growth Factor

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and is released and/or synthesized by platelets, macrophages, endothelial cells, and rat mesangial cells. In the present investigation, we found that human glomerular mesangial cells in culture release a PDGF-like protein which competes for 125I-PDGF binding to human foreskin fibroblasts and is mitogenic for these fibroblasts. The competing and to a lesser extent the mitogenic activities present in the conditioned medium are partially recognized by an anti-PDGF antibody. Northern blot analysis of poly(A)+ RNA from human mesangial cells demonstrates the expression of both PDGF A- and B-chain mRNAs. PDGF also binds to mesangial cells in a specific manner and stimulates DNA synthesis and cell proliferation. These data suggest that a PDGF-like protein secreted by mesangial cells or released from platelets, monocytes, or endothelial cells during glomerular inflammation may function as an autocrine or a paracrine growth factor for these cells. The biological role of PDGF in mediating proliferative and other inflammatory events in the glomerulus remains to be identified.

scholarly journals UII and UT in grouper: cloning and effects on the transcription of hormones related to growth control

2013 ◽  
Vol 220 (1) ◽  
pp. 35-48 ◽  
Author(s):  
Caiyun Sun ◽  
Da Duan ◽  
Bo Li ◽  
Chaobin Qin ◽  
Jirong Jia ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Cyclic Peptide ◽  
Growth Control ◽  
Hek293 Cells ◽  
Neurosecretory System ◽  
Functional Study ◽  
Urotensin Ii ◽  
Caudal Neurosecretory System ◽  
Paracrine Growth Factor ◽  
Motor Effects

Urotensin II (UII) is a cyclic peptide that was originally extracted from the caudal neurosecretory system (CNSS) of fish. UII is well known to exhibit cardiovascular, ventilatory, and motor effects in vertebrates. Studies have reported that UII exerts mitogenic effects and can act as an autocrine/paracrine growth factor in mammals. However, similar information in fish is limited. In this study, the full-length cDNAs of UII and its receptor (UT) were cloned and characterized in the orange-spotted grouper. UII and UT were expressed ubiquitously in various tissues in grouper, and particularly high levels were observed in the CNSS, CNS, and ovary. A functional study showed that UT was coupled with intracellular Ca2+ mobilization in HEK293 cells. Studies carried out using i.p. injections of UII in grouper showed the following: i) in the hypothalamus, UII can significantly stimulate the mRNA expression of ghrh and simultaneously inhibit the mRNA expression of somatostatin 1 (ss1) and ss2 3 h after injection; ii) in the pituitary, UII also significantly induced the mRNA expression of gh 6 and 12 h after injection; and iii) in the liver, the mRNA expression levels of ghr1/ghr2 and igf1/igf2 were markedly increased 12 and 3 h after the i.p. injection of UII respectively. These results collectively indicate that the UII/UT system may play a role in the promotion of the growth of the orange-spotted grouper.

X. Trefoil peptide and EGF receptor/ligand transgenic mice

2000 ◽  
Vol 278 (4) ◽  
pp. G501-G506 ◽  
Author(s):  
Andrew S. Giraud
Keyword(s):  
Growth Factor ◽  
Egf Receptor ◽  
Transforming Growth Factor ◽  
Regulatory Peptides ◽  
Genetically Engineered ◽  
Loss Of Function ◽  
Transforming Growth Factor Α ◽  
Trefoil Peptides ◽  
Trefoil Peptide ◽  
Genetically Engineered Mice

The use of genetically engineered mice with both gain-of-function and loss-of-function mutations has been particularly informative about the normal and pathophysiological actions of a number of regulatory peptides of the gastrointestinal tract. This review highlights some of the major findings pertinent to the epidermal growth factor (EGF) receptor and its ligands, particularly the major gut ligand transforming growth factor-α, as well as the trefoil peptides. Both of these peptide families have important local actions in maintaining tissue homeostasis and repair after injury, and when mechanisms governing their regulation are disrupted they may contribute to disease progression. Future applications of transgenic technology to these areas are likely to be productive in furthering our understanding of the biology of these peptides in health and disease.

KGF, rather than HGF, is a paracrine growth factor in the three-dimensional culture conditions

1998 ◽  
Vol 16 ◽  
pp. S84
Author(s):  
Makoto Tsunenaga ◽  
Yukiko Kurihara ◽  
Satoshi Amano ◽  
Toshio Nishiyama ◽  
Yasuhisa Nakayama ◽  
...  
Keyword(s):  
Growth Factor ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Three Dimensional Culture ◽  
Paracrine Growth Factor
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