scholarly journals Production of ethanol from Jerusalem artichoke by mycelial pellets

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chao Zhang ◽  
Daoji Wu ◽  
Hongqi Yang ◽  
Huixue Ren
Keyword(s):  
Lower Cost ◽  
Simultaneous Saccharification And Fermentation ◽  
Volume Fraction ◽  
Ethanol Yield ◽  
New Technology ◽  
Jerusalem Artichoke ◽  
Mycelial Pellets ◽  
Repeated Use ◽  
Easy Operation ◽  
Simultaneous Saccharification

AbstractMycelial pellets formed by Aspergillus niger A-15 were used to immobilize the ethanol producing yeast Saccharomyces cerevisiae C-15. The operation parameters, such as agitation speed, temperature and mixed proportion of strains were studied. The optimal adsorption 66.9% was obtained when speed was 80r/min, temperature was 40 °C and mixed proportion(mycelial pellets: yeasts) was 1:10. With Jerusalem artichoke flour as substrate, 12.8% (V/V) of ethanol was obtained after 48 h by simultaneous saccharification and fermentation using mycelial pellets. And mycelial pellets could tolerate 19% (volume fraction) ethanol. The above results proved that this new technology was feasible, and it had the advantages of higher ethanol yield, long service life, repeated use, easy operation and lower cost in producing ethanol.

scholarly journals Effect of Pre-hydrolysis on Simultaneous Saccharification and Fermentation of Native Rye Starch

2020 ◽  
Vol 13 (6) ◽  
pp. 923-936 ◽  
Author(s):  
Ewelina Strąk-Graczyk ◽  
Maria Balcerek
Keyword(s):  
Dry Matter ◽  
Agricultural Sector ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Matter Content ◽  
Raw Material ◽  
Process Efficiency ◽  
Dry Matter Content ◽  
Sem Images ◽  
Simultaneous Saccharification

Abstract The rising population and increasing demand for food place added pressure on the agricultural sector to maintain high process efficiency while implementing environmentally friendly methods. In this study, we investigate the effect of pre-hydrolysis of native rye starch and its influence on the yield of ethanol obtained by simultaneous saccharification and fermentation (SSF) from high gravity rye mashes with 25% and 28% w w−1 dry matter content. Fermentation was carried out in a 3-day system at a temperature of 35 ± 1 °C using the dry distillery yeast Ethanol Red (Saccharomyces cerevisiae). The characteristics of the tested raw material and changes in the native rye starch during enzymatic hydrolysis were analyzed using a scanning electron microscope (SEM). The SEM images revealed characteristic changes on the surface of the starch, which was found to have a layered structure, as well as interesting behavior by the yeast during SSF when the glucose concentration in the environment was lowered. Both in the mashes with 25% and 28% w w−1 dry matter, starch pre-hydrolysis did not significantly increase either the initial amounts of sugars available to the yeast or the fermentation efficiency and ethanol yield in comparison to the mashes without this pre-treatment.

Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

2011 ◽  
Vol 343-344 ◽  
pp. 963-967 ◽  
Author(s):  
Zhang Qiang ◽  
Anne Belinda Thomsen
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Raw Material ◽  
Wet Oxidation ◽  
Inhibition Effect ◽  
Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous saccharification and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

scholarly journals Production of Bioethanol from Selected Lignocellulosic Agrowastes

2019 ◽  
pp. 1-17
Author(s):  
Olotu Emmanuel Juwon ◽  
Olukunle Folake Oluwatoyin
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Titratable Acidity ◽  
Reducing Sugar ◽  
Corn Cobs ◽  
Orange Peels ◽  
Total Titratable Acidity ◽  
Cassava Peels ◽  
Bioethanol Yield ◽  
Simultaneous Saccharification

This study evaluated the ability of cassava peels, banana peels, orange peels and corn cobs hydrolysates to produce bioethanol. Fibre fractions analysis was carried out using standard methods. The samples were pre-treated with acid and base, followed by simultaneous saccharification and fermentation (SSF) for bioethanol production. During fermentation, pH, total titratable acidity, reducing sugar, microbial load and bioethanol yield were determined. The reducing sugar yield for Aspergillus niger and Bacillus cereus were 30.28 g and 13.35 g for corn cobs. The pH was observed to decrease during fermentation period with orange peels having the lowest pH of 2.6 after 240 hours of fermentation using A. Niger and S. cerevisiae, when B. cereus and S. Cerevisiae were used the pH was observed to be 4.10.  Total titratable acidity showed increase in all the substrates, with corn cobs having the highest when B. cereus and S. cerevisiae were used (1.62), followed by cassava peels when A. niger and S. cerevisiae were used (1.52). Highest ethanol yield following simultaneous saccharification and fermentation with A. niger and S. cerevisiae was obtained in corn cobs with 17.43 g/100 g, while orange peels gave the lowest with 8.02 g/100 g, the ethanol yield from each substrates as well as the combined substrates were significantly different at p≤ 0.05. The combined substrates (1:1:1:1) gave the highest ethanol yield of 12.44 g/100 g using A. niger and S. cerevisiae.  This study therefore revealed that A. niger had the highest bioethanol yield using corn cobs as the carbon source, therefore it could be used for mass bioethanol production.

Proper ultrasound treatment increases ethanol production from simultaneous saccharification and fermentation of sugarcane bagasse

RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 91409-91419 ◽  
Author(s):  
Rajendran Velmurugan ◽  
Aran Incharoensakdi
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Ultrasound Treatment ◽  
Fermentation Processes ◽  
Cellulase Complex ◽  
Simultaneous Saccharification

To improve the saccharification and fermentation processes, proper ultrasound was applied which resulted in the presence of cellulase complex with improved β-glucosidase ratio leading to enhanced overall ethanol yield.

scholarly journals Simultaneous Saccharification and Fermentation of Sugar Beet Pulp for Efficient Bioethanol Production

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Joanna Berłowska ◽  
Katarzyna Pielech-Przybylska ◽  
Maria Balcerek ◽  
Urszula Dziekońska-Kubczak ◽  
Piotr Patelski ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Dry Matter ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Pichia Stipitis ◽  
Sulphuric Acid Solution ◽  
Sugar Beet Pulp ◽  
Enzyme Preparations ◽  
Beet Pulp ◽  
Simultaneous Saccharification

Sugar beet pulp, a byproduct of sugar beet processing, can be used as a feedstock in second-generation ethanol production. The objective of this study was to investigate the effects of pretreatment, of the dosage of cellulase and hemicellulase enzyme preparations used, and of aeration on the release of fermentable sugars and ethanol yield during simultaneous saccharification and fermentation (SSF) of sugar beet pulp-based worts. Pressure-thermal pretreatment was applied to sugar beet pulp suspended in 2% w/w sulphuric acid solution at a ratio providing 12% dry matter. Enzymatic hydrolysis was conducted using Viscozyme and Ultraflo Max (Novozymes) enzyme preparations (0.015–0.02 mL/g dry matter). Two yeast strains were used for fermentation: Ethanol Red (S. cerevisiae) (1 g/L) andPichia stipitis(0.5 g/L), applied sequentially. The results show that efficient simultaneous saccharification and fermentation of sugar beet pulp was achieved. A 6 h interval for enzymatic activation between the application of enzyme preparations and inoculation with Ethanol Red further improved the fermentation performance, with the highest ethanol concentration reaching26.9±1.2 g/L and86.5±2.1%fermentation efficiency relative to the theoretical yield.

EVALUATION OF WASTE MUSHROOM MEDIUM AS A FERMENTABLE SUBSTRATE AND BIOETHANOL PRODUCTION

2012 ◽  
Vol 06 ◽  
pp. 745-750
Author(s):  
AI ASAKAWA ◽  
CHIZURU SASAKI ◽  
CHIKAKO ASADA ◽  
YOSHITOSHI NAKAMURA
Keyword(s):  
Steam Explosion ◽  
Lentinula Edodes ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Steam Pressure ◽  
Insoluble Residue ◽  
Steam Explosion Pretreatment ◽  
Simultaneous Saccharification ◽  
Waste Mushroom Medium

Waste Shiitake (Lentinula edodes) mushroom medium, a lignocellulosic aglicultural residue, was evaluated as a fermentable substrate. 87% of the fermentable sugars remained in the waste mushroom medium. The sugar yield of the waste mushroom medium (46.3%) was higher than that of raw mushroom medium (20.3%) after 48 h of enzymatic saccharification by Meicelase because L. edodes changed wood structure. These results indicated that the waste mushroom medium is a suitable substrate for fermentation. Next, the efficient ethanol production using steam explosion pretreatment was studied. After 30 h of simultaneous saccharification and fermentation (SSF) using Meicelase and Saccharomyces cerevisiae AM12, 20.0 g/L ethanol was produced from 100 g/L water-insoluble residue of the waste mushroom medium treated at a steam pressure of 20 atm and a steaming time of 5 min. This corresponded to an ethanol yield of 77.0% of the theoretical, i.e. 14.7 g of ethanol obtained from 100 g of waste mushroom medium.

Assessment of waxy and non-waxy corn and wheat cultivars as starch substrates for ethanol fermentation

2014 ◽  
Vol 68 (3) ◽  
Author(s):  
František Gago ◽  
Viera Horváthová ◽  
Vladimír Ondáš ◽  
Ernest Šturdík
Keyword(s):  
Fermentation Process ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Starch Content ◽  
Wheat Cultivars ◽  
Fermentation Performance ◽  
Glucose Content ◽  
Waxy Corn ◽  
Ssf Process ◽  
Simultaneous Saccharification

AbstractThe amylose/amylopectin ratio in cereal substrates is one of the parameters affecting starch hydrolysis and fermentation process. Waxy (less than 1 mass % of amylose) starch seems to be suitable for improving the fuel ethanol production. The main aim of this paper was to characterize the fermentation performance of corn and wheat waxy and non-waxy cultivars in terms of simultaneous saccharification and fermentation (SSF) as well as of the separated hydrolysis and fermentation (SHF) type. Two corn (waxy and non-waxy) and two wheat (waxy and non-waxy) cultivars were used for the comparison applying separate enzymatic hydrolysis and fermentation. In the SHF process, the glucose content was higher after saccharification in the waxy corn and wheat compared to that in non-waxy corn and wheat. In the SSF of waxy varieties, the glucose content after the pre-saccharification was also higher than in the non-waxy ones. Although the starch content did not vary significantly, differences in the glucose content after saccharification were observed. The ethanol yield obtained after the distillation of mash varied from 229.2–262.3 L per ton for the SHF fermentation, while it was in the range of 311.5–347.9 L per ton for the SSF process.

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