Reinvestigation of the type specimen of Ginkgophyllum grassetii Saporta 1875 using Reflectance Transforming Imaging

2021 ◽  
Vol 295 ◽  
pp. 104526
Author(s):  
Anne-Laure Decombeix ◽  
Ignacio H. Escapa ◽  
Dario De Franceschi ◽  
Olivier Béthoux
Keyword(s):  
Author(s):  
A.J. Tousimis ◽  
T.R. Padden

The size, shape and surface morphology of human erythrocytes (RBC) were examined by scanning electron microscopy (SEM), of the fixed material directly and by transmission electron microscopy (TEM) of surface replicas to compare the relative merits of these two observational procedures for this type specimen.A sample of human blood was fixed in glutaraldehyde and washed in distilled water by centrifugation. The washed RBC's were spread on freshly cleaved mica and on aluminum coated microscope slides and then air dried at room temperature. The SEM specimens were rotary coated with 150Å of 60:40- gold:palladium alloy in a vacuum evaporator using a new combination spinning and tilting device. The TEM specimens were preshadowed with platinum and then rotary coated with carbon in the same device. After stripping the RBC-Pt-C composite film, the RBC's were dissolved in 2.5N HNO3 followed by 0.2N NaOH leaving the preshadowed surface replicas showing positive topography.


2020 ◽  
Vol 142 ◽  
pp. 83-97
Author(s):  
A Chandran ◽  
PU Zacharia ◽  
TV Sathianandan ◽  
NK Sanil

The present study describes a new species of myxosporean, Ellipsomyxa ariusi sp. nov., infecting the gallbladder of the threadfin sea catfish Arius arius (Hamilton, 1822). E. ariusi sp. nov. is characterized by bivalvular, ellipsoid or elongate-oval myxospores with smooth spore valves and a straight suture, arranged at an angle to the longitudinal spore axis. Mature myxospores measured 10.1 ± 0.8 µm in length, 6.8 ± 0.5 µm in width and 7.7 ± 0.7 µm in thickness. Polar capsules are equal in size and oval to pyriform in shape. They are positioned at an angle to the longitudinal myxospore axis and open in opposite directions. Polar capsules measured 2.8 ± 0.3 µm in length and 2.5 ± 0.4 µm in width; polar filaments formed 4-5 coils, and extended to 32.2 ± 2.1 µm in length. Monosporic and disporic plasmodial stages attached to the wall of gallbladder. Molecular analysis of the type specimen generated a 1703 bp partial SSU rDNA sequence (MN892546), which was identical to the isolates from 3 other locations. In phylogenetic analyses, genus Ellipsomyxa appeared monophyletic and E. ariusi sp. nov. occupied an independent position in maximum likelihood and Bayesian inference trees with high bootstrap values. The overall prevalence of infection was 54.8% and multiway ANOVA revealed that it varied significantly with location, year, season, sex and size of the fish host. Histopathological changes associated with E. ariusi sp. nov. infection included swelling, vacuolation and detachment of epithelial layer, reduced mucus production and altered consistency and colour of bile. Based on the morphologic, morphometric and molecular differences with known species of Ellipsomyxa, and considering differences in host and geographic locations, the present species is treated as new and the name Ellipsomyxa ariusi sp. nov. is proposed.


2015 ◽  
Vol 42 (2) ◽  
pp. 226-235 ◽  
Author(s):  
G. N. H. Waller

Eight species of mesoplodont whales (genus Mesoplodon Gervais, 1850) named during the nineteenth century are based on valid descriptions. A checklist with the original description and type material for each of these species is provided. Additional data given may include type locality and illustrative sources, type material holding institution and type registration number(s). The only type specimen for which a record of external morphology was published relates to the 1803 stranding of Sowerby's beaked whale (Mesoplodon bidens).


2013 ◽  
Vol 40 (1) ◽  
pp. 149-156 ◽  
Author(s):  
E. Charles Nelson

Archibald Menzies landed twice at Chatham Bay, Isla del Coco, in January 1795. A small number of his herbarium specimens are extant, including the type specimen of Callicosta rugifolium (Bryophyta; Daltoniaceae) and perhaps also that of Octoea insularis (Angiospermae; Lauraceae), indicating that he was probably the first to make scientific collections on the island.


2020 ◽  
Vol 324 (2) ◽  
pp. 262-272
Author(s):  
I.V. Doronin ◽  
T.N. Dujsebayeva ◽  
K.M. Akhmedenov ◽  
A.G. Bakiev ◽  
K.N. Plakhov

The article specifies the type locality of the Steppe Ribbon Racer. The holotype Coluber (Taphrometopon) lineolatus Brandt, 1838 is stored in the reptile collection of the Zoological Institute of the Russian Academy of Sciences (ZISP No 2042). Literature sources provide different information about the type locality. A mistake has been made in the title of the work with the original species description: the western coast of the sea was indicated instead of the eastern one. The place of capture was indicated as “M. Caspium” (Caspian Sea) on the label and in the reptile inventory book of the Zoological Museum of the Academy of Sciences. The specimen was sent to the museum by G.S. Karelin. The “1842” indicated on the labels and in the inventory book cannot be the year of capture of the type specimen, just as the “1837” indicated by A.M. Nikolsky. In 1837, Karelin was in Saint Petersburg and in 1842 in Siberia. Most likely, 1837 is the year when the collection arrived at the Museum, and 1842 is the year when the information about the specimen was recorded in the inventory book (catalog) of the Zoological Museum of the Academy of Sciences. In our opinion, the holotype was caught in 1932. From Karelin’s travel notes of the expedition to the Caspian Sea in 1832, follows that the snake was recorded in two regions adjacent to the eastern coast of the Caspian Sea – Ungoza Mountain (“Mangyshlak Mountains”) and site of the Western Chink of Ustyurt between Zhamanairakty and Kyzyltas Mountains (inclusive) on the northeast coast of Kaydak Sor (“Misty Mountains”). In our article, Karelin’s route to the northeastern coast of the Caspian Sea in 1832 and photographs of these localities are given. The type locality of Psammophis lineolatus (Brandt, 1838) should be restricted to the Mangystau Region of the Kazakhstan: Ungoza Mountain south of Sarytash Gulf, Mangystau (Mangyshlak) Penninsula (44°26´ N, 51°12´ E).


2016 ◽  
Vol 3 (2) ◽  
pp. 3 ◽  
Author(s):  
Mara Calvini ◽  
Maria Stella Siori ◽  
Spartaco Gippoliti ◽  
Marco Pavia

The revised catalogue of primatological material stored in the Museo Regionale di Scienze Naturali of Torino and in the Dipartimento di Scienze della Vita e Biologia dei Sistemi of the Università degli Studi di Torino and belonging to the historical material of the Torino University is introduced. The material, 494 specimens belonging to 399 individuals of 122 taxa, is of particular importance since specimens were mainly obtained during the 19th Century and the beginning of the 20th Century. A relevant part of the collection was created by the collaborators of the Museum, among which it is worth to mention F. De Filippi, A. Borelli and E. Festa, while other material came from purchases and donations from private people or the Royal Zoological Garden of Torino. Great part of the specimens is stuffed but also the osteological materials are of particular importance, as many of them derived from the specimens before being prepared and consisting of skulls or more or less complete skeletons. After this revision, the Lectotype and Paralectotypes of <em>Alouatta</em> <em>palliata</em> <em>aequatorialis</em> have been selected, and the type-specimen of the <em>brunnea</em> variety of <em>Cebus</em> <em>albifrons</em> <em>cuscinus</em> has been recognized. In addition, some specimens of particular historical-scientific importance have also been identified and here presented for the first time.


Zootaxa ◽  
2017 ◽  
Vol 4317 (2) ◽  
pp. 379
Author(s):  
MIGUEL VENCES ◽  
JÖRN KÖHLER ◽  
FRANK GLAW

We present molecular evidence for the presence of two species morphologically similar to Spinomantis bertini in Andohahela National Park, south-eastern Madagascar, differing by 5.5−6.3% pairwise DNA sequence divergences in the mitochondrial 16S rRNA gene. One of these was observed at higher elevations of ca. 1650 m above sea level, whereas the other was found at lower elevations of ca. 715 m a.s.l., close to the type locality of S. bertini (Isaka-Ivondro), and in one other location (Andreoky, ca. 1050 a.s.l.). We herein assign these low- to mid-elevation specimens to S. bertini based on their occurrence near the type locality and general agreement in colour pattern with the type specimen of Gephyromantis bertini Guibé, 1947. The high-elevation form is described as Spinomantis beckei sp. nov. based on its molecular divergence and reciprocal monophyly with respect to S. bertini, lower expression of greenish dorsal colour and less distinct frenal stripe. Based on a comparison of published call descriptions for S. bertini and our recordings of S. beckei, we hypothesize that S. bertini has a lower note repetition rate in advertisement calls. Molecular data suggest that the S. bertini species complex is more diverse than previously recognized, with at least two more candidate species identified: S. sp. Ca7 from Ranomafana National Park, and a newly identified candidate species S. sp. Ca12 from Pic d’Ivohibe Special Reserve. 


Zootaxa ◽  
2018 ◽  
Vol 4441 (1) ◽  
pp. 137
Author(s):  
JEANNE ROBINSON ◽  
JEREMY GIBSON ◽  
HELBER ADRIÁN ARÉVALO-MALDONADO ◽  
JURATE DE PRINS ◽  
JAMES WINDMILL

Nearly a century ago, wing venation was introduced in gracillariid taxonomy as a means to diagnose closely related genera and species groups. Recent advances in non-destructive virtual micro-dissections suggest promising approaches with which to revisit the relevance of wing venation characters on historic primary type specimens. Many unique type specimens in Gracillariidae and other microlepidoptera groups preserved in museum collections are in poor condition, and over the course of history have suffered loss or damage to their abdomens. Consequently, genitalia morphology is not available for diagnoses and comparisons. In this paper we emphasize the need to include the type species and type specimens into the broader context of taxonomic studies on micro-moths in general and the family Gracillariidae in particular. The genus Caloptilia has a world-wide distribution and has been the subject of research for more than 200 years, yet the generic boundaries and groupings within the genus are still unresolved due to the lack of a reliable set of taxonomic characters obtained from the primary types. We describe a method of virtual descaling of the fore- and hindwings using the unset micro-moth type specimen of Caloptilia stigmatella Fabricius, 1781, in order to demonstrate that the study of historic and fragile type specimens and diagnoses of their internal morphological characters becomes possible by applying new and non-destructive technology. 


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