Scanning and Transmission Electron Microscopy of Human Red Blood Cells

1969 ◽  
Vol 27 ◽  
pp. 44-45
Author(s):  
A.J. Tousimis ◽  
T.R. Padden
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Blood Cells ◽  
Room Temperature ◽  
Type Specimen ◽  
New Combination ◽  
Human Red Blood Cells ◽  
Transmission Electron ◽  
Microscope Slides ◽  
Scanning Electron

The size, shape and surface morphology of human erythrocytes (RBC) were examined by scanning electron microscopy (SEM), of the fixed material directly and by transmission electron microscopy (TEM) of surface replicas to compare the relative merits of these two observational procedures for this type specimen.A sample of human blood was fixed in glutaraldehyde and washed in distilled water by centrifugation. The washed RBC's were spread on freshly cleaved mica and on aluminum coated microscope slides and then air dried at room temperature. The SEM specimens were rotary coated with 150Å of 60:40- gold:palladium alloy in a vacuum evaporator using a new combination spinning and tilting device. The TEM specimens were preshadowed with platinum and then rotary coated with carbon in the same device. After stripping the RBC-Pt-C composite film, the RBC's were dissolved in 2.5N HNO3 followed by 0.2N NaOH leaving the preshadowed surface replicas showing positive topography.

Novel Methods for Preparation and Surface Treatment of Nano-CaCO3

2013 ◽  
Vol 750-752 ◽  
pp. 336-339
Author(s):  
Fa Chao Wu ◽  
Teng Fei Shen
Keyword(s):  
Electron Microscopy ◽  
Heat Treatment ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Particle Size ◽  
Fourier Transform ◽  
Room Temperature ◽  
Transmission Electron ◽  
Reduce Energy Consumption ◽  
Scanning Electron

In this work, CaCO3 nanoparticles have been synthesized via heat-treatment of a new precursor. Effect of calcinations temperature on particle size has been investigated. The products were characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). nanoCaCO3 was modified using chloroform as solvent and fatty acid as modifier atroom temperature. The advantage of this modification is that it can be proceed at room temperature and it can reduce energy consumption.

Fabrication of a vertically aligned carbon nanotube electrode and its modification by nanostructured MnO2 for supercapacitors

2009 ◽  
Vol 81 (12) ◽  
pp. 2317-2325 ◽  
Author(s):  
Wei-De Zhang ◽  
Jin Chen
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Room Temperature ◽  
Transmission Electron ◽  
Multi Walled Carbon Nanotubes ◽  
Vertically Aligned ◽  
Walled Carbon Nanotubes ◽  
Scanning Electron

Strongly bonded arrays of vertically aligned, multi-walled carbon nanotubes (MWNTs) have been successfully grown on Ta foils, and provide a convenient basis for fabricating electrodes with high conductivity and stability. The MWNT arrays were further coated by nanostructured MnO2 through reacting with KMnO4 solution at room temperature. The morphology of the MnO2/MWNT nanocomposite was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). It was found that the MnO2 is a beehive-like nanostructure that is homogeneously and densely coated on the surface of the MWNTs. The capacitance of the MWNT electrode was significantly increased from 0.14 to 6.81 mF cm–2 after being modified with nanostructured MnO2, that is, the mass-specific capacitance of the bare and MnO2-modified MWNTs was about 33 and 446 F g–1, respectively. The MnO2/MWNT nanocomposite on Ta foils could be potential for developing a supercapacitor.

RBS, TEM and SEM Characterization of Gold Nanoclusters in TiO2(110)

2003 ◽  
Vol 792 ◽  
Author(s):  
V. Shutthanandan ◽  
Y. Zhang ◽  
C. M. Wang ◽  
J. S. Young ◽  
L. Saraf ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Room Temperature ◽  
Rutherford Backscattering Spectrometry ◽  
Gold Nanoclusters ◽  
Subsequent Annealing ◽  
Transmission Electron ◽  
Scanning Electron

ABSTRACTNucleation of gold nanoclusters in TiO2(110) single crystal using ion implantation and subsequent annealing were studied by Rutherford backscattering spectrometry /channeling (RBS/C), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Approximately 1000 Au2+/nm2 were implanted at room temperature in TiO2(110) substrates. TEM and SEM measurements reveal that rounded nanoclusters were formed during the implantation. In contrast, subsequent annealing in air for 10 hours at 1275 K promoted the formation of faceted (rectangular shaped) Au nanostructures in TiO2. RBS channeling measurements further reveled that Au atoms randomly occupied the host TiO2 lattice during the implantation. However, it appears that some Au atoms moved to the Ti lattice positions after annealing.

A Facile Approach to Synthesis and Modification of Nano-Alumina

2013 ◽  
Vol 774-776 ◽  
pp. 646-649
Author(s):  
Teng Fei Shen ◽  
Ying Juan Sun
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Particle Size ◽  
Fourier Transform ◽  
Room Temperature ◽  
Transmission Electron ◽  
Nano Alumina ◽  
Reduce Energy Consumption ◽  
Scanning Electron

In this work, nanoAlumina particles (nanoAl2O3) have been synthesized via solid state reaction. Effect of quantity of surfactant on particle size has been investigated. nanoAl2O3 was modified using chloroform as solvent and fatty acid as modifier at room temperature. The advantage of this modification is that it can be proceed at room temperature and it can reduce energy consumption. The products were characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

A polymer designed to optimize antigen localization in immunoelectron microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 790-791
Author(s):  
J.B. Olesen ◽  
C.A. Heckman ◽  
A. Lukinius
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Red Blood Cells ◽  
Immunoelectron Microscopy ◽  
Transmission Electron Microscope ◽  
Blood Cells ◽  
Room Temperature ◽  
Human Red Blood Cells ◽  
Transmission Electron ◽  
Antigen Localization

We have developed a new polymer for the purpose of retaining the maximal antigenicity of proteins in samples embedded for electron microscopy. HACH is a mixture of hydroxyhexanedial, carbohydrazide and hydrazine that polymerizes at room temperature within 24 h. Tissues embedded in the polymer were thin sectioned and the sections, mounted on formvar films, were viewed with a Zeiss IOC transmission electron microscope. The ultrastructural features of cells in HACH-embedded tissues appeared similar to those previously found in GACH-embedded tissues.The ability to preserve the antigenicity of a protein up to the polymerization stage has been tested in the following manner. Human red blood cells, from either Rh+ or Rh- donors, were first exposed to the HACH prepolymer and then rinsed and exposed to a commercially available anti-D antibody. After removal of the prepolymer, Rh+ red blood cells were clumped by anti-D antibody to the same extent as control, untreated cells (Fig. 1).

Preparation of Palladium Nano-Particles by Electrospinning and its Application in Hydrogenations

2011 ◽  
Vol 217-218 ◽  
pp. 75-78 ◽  
Author(s):  
Jian Xiang Yu ◽  
Tai Qi Liu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Activity ◽  
Room Temperature ◽  
Nano Particles ◽  
Electrospinning Technique ◽  
Transmission Electron ◽  
Scanning Electron

a novel fibrous catalyst containing palladium nano-particles was produced via electrospinning technique. The fibrous catalyst was characterized by scanning electron microscopy and transmission electron microscopy respectively, the results showed that the diameter of fiber was about 200 nm and the palladium (Pd) particles were in the range of 30~40 nm. The catalyst was tested in hydrogenations of α-olefins, cyclohexene and nitrobenzol in ethanol at room temperature under 1 atmosphere hydrogen. The results showed that the titled catalyst had high activity in the hydrogenations of α-olefins, cyclohexene and nitrobenzol.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Formation of Dislocation Channels in Neutron Irradiated Molybdenum

1972 ◽  
Vol 30 ◽  
pp. 672-673
Author(s):  
S. Mahajan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ambient Temperature ◽  
Room Temperature ◽  
Channel Formation ◽  
Early Stages ◽  
Transmission Electron ◽  
Three Stages ◽  
Two Stages ◽  
Polycrystalline Molybdenum

The evolution of dislocation channels in irradiated metals during deformation can be envisaged to occur in three stages: (i) formation of embryonic cluster free regions, (ii) growth of these regions into microscopically observable channels and (iii) termination of their growth due to the accumulation of dislocation damage. The first two stages are particularly intriguing, and we have attempted to follow the early stages of channel formation in polycrystalline molybdenum, irradiated to 5×1019 n. cm−2 (E > 1 Mev) at the reactor ambient temperature (∼ 60°C), using transmission electron microscopy. The irradiated samples were strained, at room temperature, up to the macroscopic yield point.Figure 1 illustrates the early stages of channel formation. The observations suggest that the cluster free regions, such as A, B and C, form in isolated packets, which could subsequently link-up to evolve a channel.

Comparative Study of the Fine Morphology of Sensory Receptors in Aspidogaster conchicola and Cotylaspis insignis

1972 ◽  
Vol 30 ◽  
pp. 150-151
Author(s):  
Venita F. Allison ◽  
J. E. Ubelaker ◽  
J. H. Martin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Nervous System ◽  
Osmic Acid ◽  
Sensory Receptors ◽  
Transmission Electron ◽  
Sensory Structures ◽  
Fine Morphology ◽  
Scanning Electron

It has been suggested that parasitism results in a reduction of sensory structures which concomitantly reflects a reduction in the complexity of the nervous system. The present study tests this hypothesis by examining the fine morphology and the distribution of sensory receptors for two species of aspidogastrid trematodes by transmission and scanning electron microscopy. The species chosen are an ectoparasite, Cotylaspis insignis and an endoparasite, Aspidogaster conchicola.Aspidogaster conchicola and Cotylaspis insignis were obtained from natural infections of clams, Anodonta corpulenta and Proptera purpurata. The specimens were fixed for transmission electron microscopy in phosphate buffered paraformaldehyde followed by osmic acid in the same buffer, dehydrated in an ascending series of ethanol solutions and embedded in Epon 812.

Anisotropic Membranes as Substrates for Sem

1976 ◽  
Vol 34 ◽  
pp. 444-445
Author(s):  
Thomas P. Turnbull ◽  
W. F. Bowers
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Flow ◽  
Polymer Chemistry ◽  
Surface Porosity ◽  
Transmission Electron ◽  
Pore Texture ◽  
Spongy Layer ◽  
Scanning Electron

Until recently the prime purposes of filters have been to produce clear filtrates or to collect particles from solution and then remove the filter medium and examine the particles by transmission electron microscopy. These filters have not had the best characteristics for scanning electron microscopy due to the size of the pores or the surface topography. Advances in polymer chemistry and membrane technology resulted in membranes whose characteristics make them versatile substrates for many scanning electron microscope applications. These polysulphone type membranes are anisotropic, consisting of a very thin (0.1 to 1.5 μm) dense skin of extremely fine, controlled pore texture upon a much thicker (50 to 250μm), spongy layer of the same polymer. Apparent pore diameters can be controlled in the range of 10 to 40 A. The high flow ultrafilters which we are describing have a surface porosity in the range of 15 to 25 angstrom units (0.0015-0.0025μm).

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