scholarly journals Breeding High-Grain Quality and Blast Resistant Rice Variety Using Combination of Traditional Breeding and Marker-Assisted Selection

Rice Science ◽  
2021 ◽  
Vol 28 (5) ◽  
pp. 422-426
Author(s):  
Zhou Zheng ◽  
Sun Liang ◽  
Hu Wenbin ◽  
Zhou Bin ◽  
Tao Shuhua ◽  
...  
2020 ◽  
Vol 4 (3) ◽  
pp. 668-678
Author(s):  
Mithun Saha ◽  
Md. Niuz Morshed Khan ◽  
Sujan Kumar Kundu ◽  
Md. Monirul Islam ◽  
Sabina Yasmin ◽  
...  

2014 ◽  
Vol 46 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Jung-Pil Suh ◽  
Ji-Ung Jeung ◽  
Yeon-Gyu Kim ◽  
Kshirod K. Jena ◽  
Young-Chan Cho ◽  
...  

2020 ◽  
Author(s):  
Aleena D ◽  
Padma V ◽  
Rekha G ◽  
Dilip T ◽  
Punniakotti E ◽  
...  

Abstract To combat the dreaded diseases in rice like bacterial blight and blast, host plant resistance has been advocated as a sustainable method. Through the present study, we have successfully incorporated three major bacterial blight (BB) resistance genes viz., Xa21, xa13 and xa5 into NLR3449, a high yielding, blast resistant, fine-grain type popular rice variety through marker-assisted backcross breeding. Foreground selection was carried out using PCR based, gene-specific markers viz., pTA248 (Xa21), xa13prom (xa13) and xa5FM (xa5) at each generation of backcrossing, while 127 polymorphic SSR markers spanning on 12 chromosomes were used for background selection and backcrossing was limited to two rounds. At BC2F1 generation, a single plant (NLR-87-10) with 89.9% recovery and possessing all the three bacterial blight resistance genes was forwarded to BC2F2 generation. A solitary BC2F2 plant viz., NLR-87-10-106 possessing all the three resistance genes and > 90% genome recovery was identified and advanced through selfing till BC2F4 generation by adopting pedigree method. Three best lines at BC2F4 lines, possessing high level of resistance against bacterial blight and blast and equivalent or superior to NLR 34449 in terms of yield, grain quality and agro-morphological traits have been identified and advanced for multi-location trials.


2017 ◽  
Vol 7 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Silvia Utami ◽  
Utut Widyastuti ◽  
Dwinita Utami ◽  
Ida Rosdianti ◽  
Puji Lestari

2017 ◽  
Vol 5 (2) ◽  
pp. 46
Author(s):  
M. G. Akinwale ◽  
B. O. Akinyele ◽  
A. C. Odiyi ◽  
F. N. Nwilene ◽  
O. S. Osekita ◽  
...  

High yielding rain-fed lowland rice cultivars are sensitive to complete submergence stress of more than four days. In Nigeria, rice farms on about 3.6 million ha of rain-fed lowland areas are adversely affected by submergence stress. Consequently, farmers in the major rice producing states of the country lose their entire crop to flooding each year. The identification of a major quantitative traits locus (QTL) on chromosome 9, designated as sub1, has provided the opportunity to apply marker assisted selection to develop submergence tolerant varieties. This study was undertaken to introgress sub 1 into the genetic background of Nigerian lowland rice mega variety (WITA 4) and assess the ability of WITA 4 with sub 1 to withstand submergence stress. Swarna Sub1, one of the FR13A-derived submergence tolerant varieties developed by IRRI, was crossed to WITA 4, a submergence intolerant Nigerian mega variety to produce F1 hybrid. The derived BC1F2 progenies were genotyped and BC1F3 phenotyped. The results of genotyping, using two markers, namely ART5 and SC3, revealed that 18 BC1F3 plants were found carrying Swarna Sub 1 allele of ART5 while 41 BC1F3 carried WITA 4 allele of ART5. Twenty progenies were found carrying Swarna Sub 1 allele of SC3 while 37 plants carried WITA 4 allele. Two plants carried both alleles (H). Seventeen plants were observed to carry Swarna Sub 1 alleles at both markers loci while a total of 36 progenies were observed to carry WITA 4 alleles at both markers loci. The results of the phenotypic screening showed that 12 plants recorded percentage survival above 60%. The two markers showed high selection accuracies, and selection based on the markers could satisfactorily meet the needs of breeding for submergence.


2019 ◽  
Vol 20 (14) ◽  
pp. 3519 ◽  
Author(s):  
Yusuff Oladosu ◽  
Mohd Y. Rafii ◽  
Chukwu Samuel ◽  
Arolu Fatai ◽  
Usman Magaji ◽  
...  

Drought is the leading threat to agricultural food production, especially in the cultivation of rice, a semi-aquatic plant. Drought tolerance is a complex quantitative trait with a complicated phenotype that affects different developmental stages in plants. The level of susceptibility or tolerance of rice to several drought conditions is coordinated by the action of different drought-responsive genes in relation with other stress components which stimulate signal transduction pathways. Interdisciplinary researchers have broken the complex mechanism of plant tolerance using various methods such as genetic engineering or marker-assisted selection to develop a new cultivar with improved drought resistance. The main objectives of this review were to highlight the current method of developing a durable drought-resistant rice variety through conventional breeding and the use of biotechnological tools and to comprehensively review the available information on drought-resistant genes, QTL analysis, gene transformation and marker-assisted selection. The response, indicators, causes, and adaptation processes to the drought stress were discussed in the review. Overall, this review provides a systemic glimpse of breeding methods from conventional to the latest innovation in molecular development of drought-tolerant rice variety. This information could serve as guidance for researchers and rice breeders.


2015 ◽  
Vol 10 (4) ◽  
pp. 165-169 ◽  
Author(s):  
Alexander Vyacheslavovich Usatov ◽  
Andrey Vasilevich Alabushev ◽  
Pavel Ivanovich Kostylev ◽  
Kirill Vitalievich Azarin ◽  
Maxim Stanislavovich Makarenko ◽  
...  

2016 ◽  
Vol 49 (4) ◽  
pp. 57-68 ◽  
Author(s):  
A.S. Gharieb ◽  
T.F. Metwally ◽  
S.H. Abou-Khadrah ◽  
A.A. Glela ◽  
A. El Sabagh

Abstract The present research was conducted to study the influence of organic and inorganic sources of nutrients as well as antioxidant on rice quality. The experiment was designed in split-split plots with four replications, where main plots were assigned to nitrogen (0, 55, 110 and 165 kg N ha−1) and sub plots were allocated to compost (0, 3.5 and 7 tons ha−1), while the sub-sub plots were assigned to ascobien (control, spraying with ascobien in two times at 15 and 30 days after transplanting (DAT), spraying with ascobien three times at 15, 30 and 45 DAT). The result indicated that grain quality traits were significantly influenced by the organic and inorganic fertilizers, and ascobien. The percentage of hulling, milling and amylose were positively and significantly influenced by nitrogen, organic and antioxidants application. Most of studied characters produced the highest values with the organic fertilize were combined with nitrogen and antioxidants. Application of 110 kg N ha−1, 7 t ha−1 compost and two or three spraying of ascobien, 110 kg N ha−1 or 3.5 t ha−1 compost and three times spraying and 55 kg N ha−1, 7 t ha−1 compost and two times spraying could be recommended for optimum grain quality of Sakha106 rice variety. It can be concluded that compost along with the foliar application of ascobien can be saved from 50 to110 kg N ha−1, without reducing grain quality. It can be the key to reduce the need for chemical fertilizers and decrease the cost of production with keeping healthy soil.


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