A quality control perspective on Devil's claw, Harpagophytum procumbens and H. zeyheri: phytochemical analysis and DNA barcoding

2022 ◽  
Vol 146 ◽  
pp. 90-100
Author(s):  
E. Pretorius ◽  
N.P. Mncwangi ◽  
R.M. Kabongo ◽  
W. Chen ◽  
I. Vermaak ◽  
...  
2006 ◽  
Vol 67 (13) ◽  
pp. 1372-1377 ◽  
Author(s):  
Jin Qi ◽  
Ji-Jun Chen ◽  
Zhi-Hong Cheng ◽  
Jia-Hong Zhou ◽  
Bo-Yang Yu ◽  
...  

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Giada Capecchi ◽  
Emanuele Goti ◽  
Elena Nicolai ◽  
Maria Camilla Bergonzi ◽  
Roberto Monnanni ◽  
...  

In this study HPLC analysis for the evaluation of carotenoids and DNA barcoding are reported for three different samples of Lycium cultivated in Tuscany (Italy). These two analytical methods can represent integrative methods for quality control of goji, giving also crucial information on the plant adaptation to different environments. Hence, carotenoids represent the quality markers proposed by the monograph of the European Pharmacopoeia, while DNA barcoding can differentiate between species and populations and is useful for the detection of the homogeneity of the samples.


Heliyon ◽  
2020 ◽  
Vol 6 (5) ◽  
pp. e03921
Author(s):  
Rupasree Peruru ◽  
R. Usha Rani ◽  
Jhansyrani Thatiparthi ◽  
Sunitha Sampathi ◽  
Sujatha Dodoala ◽  
...  

Author(s):  
Dudu Özkum Yavuz ◽  
Mustapha Bulama- Modu

Aims: To review the phytomedicinal researches on endemic plants of Northern Cyprus and to assess the plants of their DNA barcoding status. Study Design: A review. Methodology: This work reviewed available and accessible original articles in EBSCO, Ovid MEDLINE®, PubMed®, ScienceDirectTM, Scopus® and Web of ScienceTM databases on phytomedicinal investigations and BOLD System, MMDBD version 1.5 and GenBank® on DNA barcodes of the endemic plants of Northern Cyprus until May, 2020. Using keywords searches related to phytochemistry, biological activity and DNA barcoding, DNA Sequences and the data obtain evaluated and the information that does not meet the inclusion criteria were excluded. We believe that this information would tentatively help researchers to ethically explore these plants for their Medicinal and Aromatic potentials. Results: Only 6 of the 20 endemic plants of Northern Cyprus were phytopharmaceutically investigated, while DNA sequences of 5 were found to be deposited in the publicly accessible databases accounting for 30% and 25% of the total plants respectively. Conclusion: Endemism is related to uniqueness in features including the phytomedicinal features, thus Northern Cyprus endemic plants hold ample of such. However the results of this review showed that only few were harnessed for their medicinal properties and hence the need for their pharmacological properties and comprehensive barcoding for proper authentication, detection of adulteration, and quality control.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kannika Thongkhao ◽  
Veerachai Pongkittiphan ◽  
Thatree Phadungcharoen ◽  
Chayapol Tungphatthong ◽  
Santhosh Kumar J. Urumarudappa ◽  
...  

Abstract Cyanthillium cinereum (L.) H.Rob. is one of the most popular herbal smoking cessation aids currently used in Thailand, and its adulteration with Emilia sonchifolia (L.) DC. is often found in the herbal market. Therefore, the quality of the raw material must be considered. This work aimed to integrate macro- and microscopic, chemical and genetic authentication strategies to differentiate C. cinereum raw material from its adulterant. Different morphological features between C. cinereum and E. sonchifolia were simply recognized at the leaf base. For microscopic characteristics, trichome and pappus features were different between the two plants. HPTLC profiles showed a distinct band that could be used to unambiguously differentiate C. cinereum from E. sonchifolia. Four triterpenoid compounds, β-amyrin, taraxasterol, lupeol, and betulin, were identified from the distinct HPTLC band of C. cinereum. The use of core DNA barcode regions; rbcL, matK, ITS and psbA-trnH provided species-level resolution to differentiate the two plants. Taken together, the integration of macroscopic and microscopic characterization, phytochemical analysis by HPTLC and DNA barcoding distinguished C. cinereum from E. sonchifolia. The signatures of C. cinereum obtained here can help manufacturers to increase the quality control of C. cinereum raw material in commercialized smoking cessation products.


2012 ◽  
Vol 143 (3) ◽  
pp. 755-771 ◽  
Author(s):  
Nontobeko Mncwangi ◽  
Weiyang Chen ◽  
Ilze Vermaak ◽  
Alvaro M. Viljoen ◽  
Nigel Gericke

2014 ◽  
Author(s):  
Florian Herrmann ◽  
Michael Wink

Traditional Chinese medicine has become increasingly popular in Europe and North America.There is evidence that quality control in terms of species authentication is sometimes inappropriate. Repeated incidents of adulterations and wrong identification, some even with serious consequences have occurred recently. The necessity of a quality control for TCM drugs to avoid these incidents is given since many years. DNA barcoding was used in this study to authenticate drugs which are often used in Chinese herbal medicine. 37 plants from 28 families were identified using nucleotide sequences of the rbcL gene. Only one adulteration could be detected. Both the advantages and limitations of rbcL as a marker gene for identification were analysed and discussed. We could show that DNA barcoding is a valid and fast method to identify medicinal herbs, showing some advantages over chemical profiling because of its universal application even for unknown plant species.


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