Objective.To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY;Vaccinium macrocarpon).Methods.Resin samples were prepared (Bis-GMA/TEGDMA at 70/30 mol%) and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX), except for the control group. Samples were individually immersed in a bacterial culture (Streptococcus mutans) for 24 h. Cell viability (n=3) was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS) and elastic modulus (E) were tested on a universal testing machine (n=8). Compound release and chemical stability were evaluated by UV spectrophotometry and1H NMR (n=3). Data were analyzed by one-way ANOVA and Tukey’s test (α= 0.05).Results.Both compounds inhibited S. mutans growth, with CHX being most effective (P<0.05). Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties (P<0.05). The 24 h compound release rates were ACY: 1.33 μg/mL and CHX: 1.92 μg/mL.1H NMR spectra suggests that both compounds remained stable after being released in water.Conclusion.The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials.
Bacterial Growth Inhibition and Antioxidant Potentials of Leaf Infusions of (Moringa oleifera), locust beans (Parkia biglobosa) and bitter leaf (Vernonia amygladina)
