Heterotrophic nitrification and aerobic denitrification process: Promising but a long way to go in the wastewater treatment

2022 ◽  
Vol 805 ◽  
pp. 150212
Author(s):  
Haipeng Xi ◽  
Xiangtong Zhou ◽  
Muhammad Arslan ◽  
Zhijun Luo ◽  
Jing Wei ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Denitrification Process

Screening and Characterization of a Bacterium Capable of Simultaneous Heterotrophic Nitrification and Aerobic Denitrification at High Concentrations of Ammonia-Nitrogen

2014 ◽  
Vol 665 ◽  
pp. 487-490
Author(s):  
Te Wang ◽  
Zhao Xia Liu ◽  
Mei Juan Wu ◽  
Fu Hui Kang ◽  
Qing Chen ◽  
...  
Keyword(s):  
16S Rdna ◽  
Ammonia Nitrogen ◽  
Heterotrophic Nitrification ◽  
Denitrification Rate ◽  
Aerobic Denitrification ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Mass Ratios ◽  
High Concentrations ◽  
Denitrification Process

A bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of ammonia-nitrogen was screened and identified and the denitrification property was investigated in this paper. The strain was isolated from aeration tank of wastewater disposed by activated sludge and analyzed and identified by 16S rDNA. The effects of different carbon sources and carbon and nitrogen mass ratios on denitrification rate were studied. The changes of various forms of ammonia-nitrogens during the simultaneous heterotrophic nitrification and aerobic denitrification process were characterized. A strain capable of simultaneous heterotrophic nitrification and aerobic denitrification at 600 mg/L nitrogen concentration has been isolated and screened. Comparison of its 16S rDNA sequence showed 100% similarity to Bacillus licheniformis strain Lr124/6. The strain was named as Bacillus sp. A22. The optimal conditions for degradation of ammonia-nitrogen by Bacillus sp. A22 were trisodium citrate as carbon source and carbon and nitrogen mass ratios of 10. The denitrification rate was 98.2% after 96 h of culture under the optimal conditions and there was hardly any intermediates accumulation in the denitrification process. It has practical applications that the denitrification can be performed efficiently at high concentrations of ammonia-nitrogen by method of simultaneous heterotrophic nitrification and aerobic denitrification by Bacillus sp. A22 in nitrogen purification treatment of wastewater with high concentrations of ammonia-nitrogen.

Microbiological conversions in nitrogen removal

1998 ◽  
Vol 38 (1) ◽  
pp. 1-7 ◽  
Author(s):  
M. C. M. Van Loosdrecht ◽  
M. S. M. Jetten
Keyword(s):  
Wastewater Treatment ◽  
Nitrogen Removal ◽  
Organic Substrate ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Ammonium Oxidation ◽  
Treatment Processes ◽  
Nitrogen Conversion ◽  
The Many ◽  
Wastewater Treatment Systems

Nitrogen conversion processes are essential for most wastewater treatment systems. Due to the many possible conversions, and the complexity of analysing the reactions under actual conditions, there is much room for confusion. This review provides an overview of the possible microbiological nitrogen conversions described in literature. The relevance of these conversions with respect to wastewater treatment processes is discussed. Rates described for aerobic denitrification or denitrification by autotrophic nitrifiers are so low that these conversions probably do not play a significant role under practical conditions. Heterotrophic nitrification may be of relevance only when the wastewater contains a high COD/N ratio (>10). Anaerobic ammonium oxidation can occur in fully autotrophic systems with very long sludge retention times or biofilm systems. This conversion offers great opportunities since it allows us to denitrify with ammonium as electron donor, i.e. no organic substrate is needed in the nitrogen removal process.

scholarly journals Heterotrophic nitrification and aerobic denitrification using pure-culture bacteria for wastewater treatment

2018 ◽  
Vol 9 (1) ◽  
pp. 10-17 ◽  
Author(s):  
W. Khanichaidecha ◽  
A. Nakaruk ◽  
K. Ratananikom ◽  
R. Eamrat ◽  
F. Kazama
Keyword(s):  
Wastewater Treatment ◽  
Nitrogen Removal ◽  
Pure Culture ◽  
Water Reuse ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Nitrification Rate ◽  
Wastewater Reclamation ◽  
Treatment Unit ◽  
Term Operation

Abstract Due to the high water demand and unsustainable water resource, wastewater reclamation and wastewater treatment prior to discharge have become current important issues. Various treatment technologies, such as biological processes, have been improved as alternatives. In this study, the biological nitrogen removal system using pure-culture Bacillus licheniformis was developed and used as an internal treatment unit in an aquarium to improve the effluent quality for water reuse. The efficiencies for NH4-N and total nitrogen (TN) removal and the quality of treated water verified the occurrence of heterotrophic nitrification and aerobic denitrification; the nitrification rate was 0.84 mg/L-h and the denitrification rate was 0.62 mg/L-h. The maximal NH4-N and TN removal efficiencies were approximately 73% at the influent NH4-N of 30 mg/L. However, the other competitive heterotroph of Pseudomonas sp. was observed, which resulted in dramatically decreasing efficiencies and an enlarged ratio of carbon consumption and nitrogen removal. Although the overall performance of the B. licheniformis system was lower than the system using mixed-culture nitrifying and heterotrophic denitrifying microorganisms, the advantages of the B. licheniformis system were ease of operation and the fact that it is a land-limited treatment system. The research is ongoing to enhance performance and maintain excellent efficiency in a long-term operation.

scholarly journals Formulation and Characterization of a Heterotrophic Nitrification-Aerobic Denitrification Synthetic Microbial Community and its Application to Livestock Wastewater Treatment

Water ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 218 ◽  
Author(s):  
Qi-yu Zhang ◽  
Ping Yang ◽  
Lai-sheng Liu ◽  
Zeng-jin Liu
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Carbon Source ◽  
Batch Reactor ◽  
Treatment Plant ◽  
Ammonia Nitrogen ◽  
Ammonia Removal ◽  
Heterotrophic Nitrification ◽  
Aerobic Denitrification ◽  
Livestock Wastewater

There have been many studies on single strains in wastewater treatment and a new synthetic microbial community was prepared in this study, which provides a reference for the application of heterotrophic nitrification-aerobic denitrification in actual wastewater treatment. The growth period distribution of the composite bacteria was determined by plotting growth curves with different sole nitrogen sources, and the influence of the carbon source, carbon to nitrogen ratio (C/N) ratio, pH, and temperature on ammonia removal by the composite heterotrophic nitrifying-aerobic denitrifying strain was investigated. The optimal conditions for the heterotrophic nitrification process were sodium citrate as the carbon source, a C/N ratio of 10, a pH of 7, and a temperature of 30 °C, and only trace amounts of nitrate and nitrite were observed during the process. When the sequencing batch reactor (SBR) of a pig farm wastewater treatment plant was inoculated with the synthetic microbial community, the average removals of the chemical oxygen demand (COD) and ammonia nitrogen in the effluent were 92.61% and 20.56%, respectively. From the results, the synthetic microbial community was able to simultaneously perform heterotrophic nitrification-aerobic denitrification indicating great potential for full-scale applications.

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