A comprehensive review on carbon source effect of microalgae lipid accumulation for biofuel production

Abstract Background: Cutaneotrichosporon oleaginosus ATCC 20509 is a fast growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. Results: To increase our understanding of this yeast and to provide a knowledge base for further industrial use, a FAIR re-annotated genome was used to build a genome-scale, constraint-based metabolic model containing 1553 reactions involving 1373 metabolites in 11 compartments. A new description of the biomass synthesis reaction was introduced to account for massive lipid accumulation in conditions with high carbon to nitrogen (C/N) ratio in the media. This condition-speciﬁc biomass objective function is shown to better predict conditions with high lipid accumulation using glucose, fructose, sucrose, xylose, ethanol and glycerol as sole carbon source. Conclusion: Contributing to the economic viability of biodiesel as renewable fuel, C. oleaginosus ATCC 20509 can eﬀectively convert crude glycerol waste streams in lipids as a potential bioenergy source. Performance simulations are essential to identify optimal production conditions and to develop and ﬁne tune a cost-eﬀective production process. Our model suggests ATP-citrate lyase as a target for overexpression to further improve lipid production.

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Genome-scale metabolic modelling underscores the potential of Cutaneotrichosporon oleaginosus ATCC 20509 as a cell factory for biofuel production

10.21203/rs.3.rs-26414/v2 ◽

2020 ◽

Author(s):

Nhung TT Pham ◽

Maarten Reijnders ◽

Maria Suarez-Diez ◽

Bart Nijsse ◽

Jan Springer ◽

...

Keyword(s):

Carbon Source ◽

Lipid Accumulation ◽

Crude Glycerol ◽

Metabolic Model ◽

Biodiesel Production ◽

Biofuel Production ◽

Cell Factory ◽

A Genome ◽

Wide Range ◽

Genome Scale

Abstract Background: Cutaneotrichosporon oleaginosus ATCC 20509 is a fast growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. Results: To increase our understanding of this yeast and to provide a knowledge base for further industrial use, a FAIR re-annotated genome was used to build a genome-scale, constraint-based metabolic model containing 1553 reactions involving 1373 metabolites in 11 compartments. A new description of the biomass synthesis reaction was introduced to account for massive lipid accumulation in conditions with high carbon to nitrogen (C/N) ratio in the media. This condition-speciﬁc biomass objective function is shown to better predict conditions with high lipid accumulation using glucose, fructose, sucrose, xylose and glycerol as sole carbon source. Conclusion: Contributing to the economic viability of biodiesel as renewable fuel, C. oleaginosus ATCC 20509 can eﬀectively convert crude glycerol waste streams in lipids as a potential bioenergy source. Performance simulations are essential to identify optimal production conditions and to develop and ﬁne tune a cost-eﬀective production process. Our model suggests ATP-citrate lyase as a target for further improve lipid production. Keywords: Genome-scale metabolic model; Cutaneotrichosporon oleaginosus ATCC 20509; lipid accumulation; Crude glycerol; biodiesel production; ﬂux balance analysis; oleaginous yeast

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Genome-scale metabolic modeling underscores the potential of Cutaneotrichosporon oleaginosus ATCC 20509 as a cell factory for biofuel production

Biotechnology for Biofuels ◽

10.1186/s13068-020-01838-1 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Nhung Pham ◽

Maarten Reijnders ◽

Maria Suarez-Diez ◽

Bart Nijsse ◽

Jan Springer ◽

...

Keyword(s):

Carbon Source ◽

Lipid Accumulation ◽

Crude Glycerol ◽

Unsaturated Fatty Acids ◽

Biodiesel Production ◽

Biofuel Production ◽

Cell Factory ◽

A Genome ◽

Wide Range ◽

Genome Scale

Abstract Background Cutaneotrichosporon oleaginosus ATCC 20509 is a fast-growing oleaginous basidiomycete yeast that is able to grow in a wide range of low-cost carbon sources including crude glycerol, a byproduct of biodiesel production. When glycerol is used as a carbon source, this yeast can accumulate more than 50% lipids (w/w) with high concentrations of mono-unsaturated fatty acids. Results To increase our understanding of this yeast and to provide a knowledge base for further industrial use, a FAIR re-annotated genome was used to build a genome-scale, constraint-based metabolic model containing 1553 reactions involving 1373 metabolites in 11 compartments. A new description of the biomass synthesis reaction was introduced to account for massive lipid accumulation in conditions with high carbon-to-nitrogen (C/N) ratio in the media. This condition-specific biomass objective function is shown to better predict conditions with high lipid accumulation using glucose, fructose, sucrose, xylose, and glycerol as sole carbon source. Conclusion Contributing to the economic viability of biodiesel as renewable fuel, C. oleaginosus ATCC 20509 can effectively convert crude glycerol waste streams in lipids as a potential bioenergy source. Performance simulations are essential to identify optimal production conditions and to develop and fine tune a cost-effective production process. Our model suggests ATP-citrate lyase as a possible target to further improve lipid production.

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Evaluation of different fungi and bacteria strains for production of cellulases by submerged fermentation using sugarcane bagasse as carbon source: Effect of substrate concentration and cultivation temperature

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2020.17210 ◽

2020 ◽

Vol 19 (9) ◽

pp. 625-635

Author(s):

M. Pinotti Laura ◽

B. Paulino Larissa ◽

C. Agnezi Jacyele ◽

A. dos Santos Patrícia ◽

N. L. da Silva Henrique ◽

...

Keyword(s):

Carbon Source ◽

Sugarcane Bagasse ◽

Substrate Concentration ◽

Submerged Fermentation ◽

Cultivation Temperature ◽

Source Effect

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Biodiesel production from heterotrophic oleaginous microalga Thraustochytrium sp. BM2 with enhanced lipid accumulation using crude glycerol as alternative carbon source

Bioresource Technology ◽

10.1016/j.biortech.2020.123113 ◽

2020 ◽

Vol 306 ◽

pp. 123113 ◽

Cited By ~ 8

Author(s):

Chun-Yen Chen ◽

Meng-Hsiu Lee ◽

Yoong Kit Leong ◽

Jo-Shu Chang ◽

Duu-Jong Lee

Keyword(s):

Carbon Source ◽

Lipid Accumulation ◽

Crude Glycerol ◽

Biodiesel Production ◽

Alternative Carbon Source

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Lipid accumulation capability of typical non‐acclimated activated sludge microbial consortia using methane gas as secondary carbon source

Engineering Reports ◽

10.1002/eng2.12148 ◽

2020 ◽

Vol 2 (4) ◽

Author(s):

Dhan Lord B. Fortela ◽

Wayne Sharp ◽

Emmanuel Revellame ◽

Andrei Chistoserdov ◽

William Holmes ◽

...

Keyword(s):

Carbon Source ◽

Activated Sludge ◽

Lipid Accumulation ◽

Microbial Consortia ◽

Methane Gas ◽

Acclimated Activated Sludge ◽

Secondary Carbon

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N-Acetylglucosamine Utilization by Saccharomyces cerevisiae Based on Expression of Candida albicans NAG Genes

Applied and Environmental Microbiology ◽

10.1128/aem.00053-09 ◽

2009 ◽

Vol 75 (18) ◽

pp. 5840-5845 ◽

Cited By ~ 34

Author(s):

Jürgen Wendland ◽

Yvonne Schaub ◽

Andrea Walther

Keyword(s):

Saccharomyces Cerevisiae ◽

Candida Albicans ◽

Carbon Source ◽

Sole Carbon Source ◽

De Novo ◽

Biofuel Production ◽

Bioethanol Production ◽

Catabolic Pathway ◽

Cellular Functions ◽

Kinase A

ABSTRACT Synthesis of chitin de novo from glucose involves a linear pathway in Saccharomyces cerevisiae. Several of the pathway genes, including GNA1, are essential. Genes for chitin catabolism are absent in S. cerevisiae. Therefore, S. cerevisiae cannot use chitin as a carbon source. Chitin is the second most abundant polysaccharide after cellulose and consists of N-acetylglucosamine (GlcNAc) moieties. Here, we have generated S. cerevisiae strains that are able to use GlcNAc as a carbon source by expressing four Candida albicans genes (NAG3 or its NAG4 paralog, NAG5, NAG2, and NAG1) encoding a GlcNAc permease, a GlcNAc kinase, a GlcNAc-6-phosphate deacetylase, and a glucosamine-6-phosphate deaminase, respectively. Expression of NAG3 and NAG5 or NAG4 and NAG5 in S. cerevisiae resulted in strains in which the otherwise-essential ScGNA1 could be deleted. These strains required the presence of GlcNAc in the medium, indicating that uptake of GlcNAc and its phosphorylation were achieved. Expression of all four NAG genes produced strains that could use GlcNAc as the sole carbon source for growth. Utilization of a GlcNAc catabolic pathway for bioethanol production using these strains was tested. However, fermentation was slow and yielded only minor amounts of ethanol (approximately 3.0 g/liter), suggesting that fructose-6-phosphate produced from GlcNAc under these conditions is largely consumed to maintain cellular functions and promote growth. Our results present the first step toward tapping a novel, renewable carbon source for biofuel production.

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Evaluating the Potential of Rhodosporidium toruloides-1588 for High Lipid Production Using Undetoxified Wood Hydrolysate as a Carbon Source

Energies ◽

10.3390/en13225960 ◽

2020 ◽

Vol 13 (22) ◽

pp. 5960

Author(s):

Rahul Saini ◽

Krishnamoorthy Hegde ◽

Carlos Saul Osorio-Gonzalez ◽

Satinder Kaur Brar ◽

Pierre Vezina

Keyword(s):

Carbon Source ◽

Low Cost ◽

Lipid Production ◽

Rhodosporidium Toruloides ◽

Biofuel Production ◽

Sugar Consumption ◽

High Lipid ◽

Lipid Characterization ◽

Forestry Residues ◽

Wood Hydrolysate

The study aims to explore microbial lipid production using an abundant and low-cost lignocellulosic biomass derived from forestry residues. Sugar-rich undetoxified hydrolysate was prepared using hardwood and softwood sawdust and used for lipid production as a carbon source from an oleaginous yeast, Rhodosporidium toruloides-1588. The maximum biomass obtained was 17.09 and 19.56 g/L in hardwood and softwood hydrolysate, respectively. Sugar consumption in both hydrolysates was >95%, with a maximum lipid accumulation of 36.68% at 104 h and 35.24% at 96 h. Moreover, R. toruloides-1588 exhibited tolerance to several toxic compounds such as phenols, organic acids and furans present in hydrolysates. The lipid characterization showed several monosaturated and polyunsaturated fatty acids, making it a potential feedstock for biofuels and oleochemicals production. This study confirms the credibility of R. toruloides-1588 as a suitable lipid producer using hydrolysates from forestry residues as a substrate. Additionally, lipids obtained from R. toruloides-1588 could be a potential feedstock for advanced biofuel production as well as for food and pharmaceutical applications.

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