Evaluation of different fungi and bacteria strains for production of cellulases by submerged fermentation using sugarcane bagasse as carbon source: Effect of substrate concentration and cultivation temperature

AbstractThe high cost of commercial cellulases still hampers the economic competitiveness of the production of fuels and chemicals from lignocellulosic biomasses. This cost may be decreased by the on-site production of cellulases with the integrated use of the lignocellulosic biomass as carbon source. This integrated approach was evaluated in the present study whereby steam-pretreated sugarcane bagasse (SPSB) was used as carbon source for the production of cellulases by Trichoderma reesei Rut C30 and the produced enzymes were subsequently used for SPSB hydrolysis. An enzyme preparation with a high cellulase activity, of 1.93 FPU/mL, was obtained, and a significant β-glucosidase activity was achieved in buffered media, indicating the importance of pH control during enzyme production. The hydrolysis of SPSB with the laboratory-made mixture resulted in a glucose yield of 80%, which was equivalent to those observed for control experiments using commercial enzymes. Even though the supplementation of this mixture with external β-glucosidase from Aspergillus awamori was found to increase the initial hydrolysis rates, it had no impact on the final hydrolysis yield. It was shown that SPSB is a promising carbon source for the production of cellulases and β-glucosidases by T. reesei Rut C30 and that the enzyme preparation obtained is effective for the hydrolysis of SPSB, supporting the on-site integrated approach to decrease the cost of the enzymatic hydrolysis of lignocellulosic biomass.

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Studies on Nisin Production by Isolated Lactobacillus Species in Aqueous Two-phase System

Mapana Journal of Sciences ◽

10.12723/mjs.10.6 ◽

2007 ◽

Vol 6 (1) ◽

pp. 69-80

Author(s):

Namita Singh ◽

S. K. Mandal

Keyword(s):

Carbon Source ◽

Submerged Fermentation ◽

Biomass Yield ◽

Cheese Whey ◽

Maximum Yield ◽

Lactobacillus Species ◽

Phase System ◽

Two Phase ◽

Nisin Production ◽

Aqueous Two Phase System

Isolated lactobacillus sp.(from cheese whey) was examined for its ability to produce bacteriocin like nisin in submerged fermentation and aqueous two-phase system(ATPS) at pH 6.5, 30.C for 18 h fermentation using M17S medium with 5%(v/v) inoculum and the nisin yields were obtained 4500 IU/ml and 20216 IU/ml where as biomass yield obtained 19.55 mg/ml and 31.88 mg/ml respectively.The maximum yield of nisin (24800 IU/ml) and biomass (22.49 mg/ml) were obtained in ATPS (28% PEG 6000 and 4% MgSO4.7H20 with 5-times concentrated M17 medium containing 0.5% lactose as carbon source) under similar conditions.

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Multiplication Characteristics of FRNA Phage and Its Utility as an Indicator for Pathogenic Viruses

Water Science & Technology ◽

10.2166/wst.1993.0335 ◽

1993 ◽

Vol 27 (3-4) ◽

pp. 133-136 ◽

Cited By ~ 4

Author(s):

Naoyuki Kamiko ◽

Shinichiro Ohgaki

Keyword(s):

Bacterial Growth ◽

Growth Phase ◽

Substrate Concentration ◽

Pure Culture ◽

Environmental Samples ◽

Cultivation Temperature ◽

Pathogenic Viruses ◽

Phage Multiplication ◽

Rna Phage

The possibility of multiplication of F-specific RNA phage (FRNA phage) multiplication in the environment was investigated. Using the Qβ strain in pure culture as a model FRNA phage, the effects of bacterial growth phase, substrate concentration and cultivation temperature on phage multiplication were studied. Similar experiments with environmental samples from raw sewage were then preformed.

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Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Hoehnea ◽

10.1590/2236-8906-40/2017 ◽

2018 ◽

Vol 45 (1) ◽

pp. 134-142 ◽

Cited By ~ 5

Author(s):

Flaviane Lopes Ferreira ◽

Cesar Barretta Dall'Antonia ◽

Emerson Andrade Shiga ◽

Larissa Juliani Alvim ◽

Rosemeire Aparecida Bom Pessoni

Keyword(s):

Carbon Source ◽

Enzymatic Activity ◽

Sugarcane Bagasse ◽

Enzyme Production ◽

Sole Carbon Source ◽

Sole Source ◽

Maximum Activity ◽

Liquid Media ◽

Biotechnological Potential ◽

Efficient Producer

ABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.

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