Inclusion as an efficient purification method for specific removal of tricyclic organic sulfur/nitrogen pollutants in fuel and effluent with cyclodextrin polymers

2021 ◽  
Vol 254 ◽  
pp. 117643
Author(s):  
Zunbin Duan ◽  
Shengchao Wei ◽  
He Bian ◽  
Chengdong Guan ◽  
Lijun Zhu ◽  
...  
Keyword(s):  
Organic Sulfur ◽  
Purification Method ◽  
Cyclodextrin Polymers ◽  
Nitrogen Pollutants

Uultrastructure of Microtubules

1972 ◽  
Vol 30 ◽  
pp. 252-253
Author(s):  
Ariaki Nagayama
Keyword(s):  
Fine Structure ◽  
Cultured Cells ◽  
Present Report ◽  
Confluent Monolayer ◽  
Purification Method ◽  
Vinca Rosea ◽  
L Cells ◽  
Antimitotic Activity ◽  
Monolayer Cultures ◽  
Rapid Purification

Vinblastine(Vb) or vincristine, alkaloid derived from Vinca rosea is known for its antimitotic activity by regrouping of microtubules into paracrystalline form within the cells. A rapid purification method of vinblastine-induced microtubular paracrystals(PC) has provided us with a fresh and pure microtubular material demonstrating the presence of a labile ATPase associated with the PC. The present report is concerned with the fine structure of purified microtubules of mammalian cultured cells.Confluent monolayer cultures of L cells were incubated for 20hrs with 10-5 M Vb (donated from Shionogi Seiyaku & Co., Osaka, Japan).

New purification method for vitamin A derived aging pigments

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
E Jee ◽  
S Kim ◽  
Y Jang
Keyword(s):  
Vitamin A ◽  
Purification Method

Isolation of Human Fibrinogen and its Derivatives by Affinity Chromatography on Gly-Pro-Arg-Pro-Lys-Fractogel

1990 ◽  
Vol 63 (03) ◽  
pp. 439-444 ◽  
Author(s):  
C Kuyas ◽  
A Haeberli ◽  
P Walder ◽  
P W Straub
Keyword(s):  
Gel Electrophoresis ◽  
Affinity Purification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Ease Of Use ◽  
Terminal Sequence ◽  
Purification Method ◽  
Human Fibrinogen ◽  
Isolation Methods ◽  
One Step ◽  
Complementary Binding

SummaryWith an immobilized synthetic pentapeptide GlyProArgProLys comprising the N-terminal sequence GlyProArg of the α-chain of fibrin, a new affinity method for the quantitative isolation of fibrinogen out of anticoagulated plasma was developed. The method proved to be superior to all known isolation methods in respect to ease of use and yield, since fibrinogen could be isolated in one step out of plasma with a recovery of more than 95% when compared to the immunologically measurable amounts of fibrinogen. Moreover the amounts of contaminating proteins such as fibronectin, factor XIII or plasminogen were negligible and the purity of the isolated fibrinogen was higher than 95% as measured by polyacrylamide gel electrophoresis. The clottability was 90% and more. Another advantage of this affinity purification method is the possibility to isolate fibrinogen quantitatively out of small plasma samples (<5 ml). Further, abnormal fibrinogen molecules, provided their complementary binding site for GlyProArg is preserved, may also be quantitatively isolated independent of any solubility differences as compared to normal fibrinogen. In addition fibrin(ogcn) fragments originating from plasmic digestion can be separated on the basis of their affinity to GlyProArg. The described affinity gel can be used more than 50 times without any loss of capacity.

Use of Factor VIII in the Treatment of Hemophilia

1962 ◽  
Vol 07 (02) ◽  
pp. 230-238 ◽  
Author(s):  
A Pavlovsky ◽  
H Peterson ◽  
G Casillas ◽  
C Simonetti ◽  
A Martinez Canaveri ◽  
...  
Keyword(s):  
Factor Viii ◽  
Tannic Acid ◽  
Deae Cellulose ◽  
Fibrinolytic System ◽  
Purification Method ◽  
Fraction I

SummaryThis publication describes the results obtained by treatment of haemophiliacs with factor VIII preparations isolated from Cohn fraction I by use of tannic acid, FI-O-Ta.The authors stress the rapidity of the disappearance of factor VIII after injection. Transfusions are generally well tolerated. One reaction of the pyrogen type has been observed and also a case of activation of the fibrinolytic system.A second purification method by means of chromatography on DEAE-cellulose is described.

A Novel Method for the Rapid Purification of Human and Rat Fibrin(OGEN) Degradation Products in High Yields

1979 ◽  
Author(s):  
W. Nieuwenhuizen ◽  
I. A. M. van Ruijven-Vermeer ◽  
F. Haverkate ◽  
G. Timan
Keyword(s):  
Degradation Products ◽  
Complete Separation ◽  
Purification Method ◽  
Amino Terminal ◽  
Novel Method ◽  
Rapid Purification ◽  
The One ◽  
High Yields ◽  
Size Heterogeneity ◽  
D Dimer

A novel method will be described for the preparation and purification of fibrin(ogen) degradation products in high yields. The high yields are due to two factors. on the one hand an improved preparation method in which the size heterogeneity of the degradation products D is strongly reduced by plasmin digestion at well-controlled calcium concentrations. At calcium concentrations of 2mM exclusively D fragments, M.W.= 93-000 (Dcate) were formed; in the presence of 1OmM EGTA only fragments M.W.= 80.000 (D EGTA) were formed as described. on the other hand a new purification method, which includes Sephadex G-200 filtration to purify the D:E complexes and separation of the D and E fragments by a 16 hrs. preparative isoelectric focussing. The latter step gives a complete separation of D (fragments) (pH = 6.5) and E fragments (at pH = 4.5) without any overlap, thus allowing a nearly 100% recovery in this step. The overall recoveries are around 75% of the theoretical values. These recoveries are superior to those of existing procedures. Moreover the conditions of this purification procedure are very mild and probably do not affect the native configuration of the products. Amino-terminal amino acids of human Dcate, D EGTA and D-dimer are identical i.e. val, asx and ser. in the ratgly, asx and ser were found. E 1% for rat Dcate=17-8 for rat D EGTA=16.2 and for rat D- dimer=l8.3. for the corresponding human fragments, these values were all 20.0 ± 0.2.

β-Cyclodextrin Polymers on Microcrystalline Cellulose as a Granular Media for Organic Micropollutant Removal from Water

2018 ◽  
Author(s):  
Diego Alzate-Sanchez ◽  
Yuhan Ling ◽  
Chenjun Li ◽  
Benjamin Frank ◽  
Reiner Bleher ◽  
...  
Keyword(s):  
Microcrystalline Cellulose ◽  
Continuous Flow ◽  
Granular Media ◽  
Anaerobic Biodegradation ◽  
Thin Coating ◽  
Micropollutant Removal ◽  
Cyclodextrin Polymers ◽  
Flow Experiments ◽  
Aerobic And Anaerobic ◽  
Cellulose Composite

This manuscript describes cyclodextrin polymers formed as a thin coating on microcrystalline cellulose. The resulting polymer/cellulose composite shows promising performance for removing organic pollutants from water and can be packed into columns for continuous-flow experiments. The polymer/cellulose composite also shows excellent resistance to aerobic and anaerobic biodegradation.

Crosslinker Chemistry Determines the Uptake Potential of Perfluorinated Alkyl Substances by β-Cyclodextrin Polymers

2018 ◽  
Author(s):  
Leilei Xiao ◽  
Casey Ching ◽  
Yuhan Ling ◽  
Mohammadreza Nasiri ◽  
Max Justin Klemes ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Polymer Networks ◽  
Cyclodextrin Polymer ◽  
Perfluorinated Alkyl Substances ◽  
Cyclodextrin Polymers

This work describes several crosslinked β-cyclodextrin polymer networks and correlates the crosslinker chemistry with binding affinity for per- and polyfluorinated alkyl substances (PFASs), including PFOA and PFOS.

Experience in Implementation of Systems Applied for Drainage Water Ion-Selective Purification from Radionuclides at Units 1,2 of Novovoronezh-2 NPP

ANRI ◽  
2020 ◽  
Vol 0 (4) ◽  
pp. 64-70
Author(s):  
Vladimir Povarov ◽  
Igor' Gusev ◽  
Sergey Rosnovsky ◽  
Dmitriy Statsura ◽  
Vladimir Kazansky ◽  
...  
Keyword(s):  
Water Purification ◽  
Drainage Water ◽  
Purification Method ◽  
Selective Sorbent ◽  
Iron Based ◽  
Purification Plant ◽  
Nickel Ferrocyanide ◽  
Satisfactory Quality

The article is dedicated to the assessment of the experience in drainage water purification from radionuclides using ion-selective purification method on Units 1,2 Novovoronezh-2 NPP. Application of an ion-selective sorbent based on nickel ferrocyanide, as well as the preliminary oxidation of corrosive origin radiongjuclides, allow to achieve a satisfactory quality of purification from Cs-134, Cs-137, Co-60, Co 58, Mn-54, Cr-51. However, this method turned out to be ineffective for drainage water purification from Be-7, Sb-124, Sb-125. The article presents a possible method for drainage water purification from Be-7, Sb-124, Sb-125 using an iron-based coagulant, prospects for the modernization of the ion-selective drainage water purification plant, as well as plans to improve methods for drainage water purification at Novovoronezh-2 NPP

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