scholarly journals Protective effect of Bosutinib with caspase inhibitors on human K562 cell lines

2021 ◽  
Author(s):  
Roua S. Baty
Keyword(s):  
Protective Effect ◽  
Cell Lines ◽  
K562 Cell ◽  
Caspase Inhibitors

scholarly journals Anti-K562 cell monoclonal antibody RTF8X recognizes tumor-associated antigen of erythroid lineage

Blood ◽  
1988 ◽  
Vol 72 (5) ◽  
pp. 1487-1491 ◽  
Author(s):  
T Sakurai ◽  
H Hara ◽  
K Nagai
Keyword(s):  
Monoclonal Antibody ◽  
Cell Lines ◽  
Surface Antigen ◽  
K562 Cell ◽  
K562 Cells ◽  
Erythroid Differentiation ◽  
Enzyme Treatment ◽  
Normal Peripheral Blood ◽  
Marrow Cells

Abstract A new anti-K562 cell monoclonal antibody, RTF8X, a cytotoxic IgM, recognized a surface antigen on erythroblasts from patients with erythroleukemia and polycythemia vera. RTF8X, which is highly specific to K562 cells, did not react with the other 14 hematopoietic cell lines and the seven nonhematopoietic cell lines. RTF8X antigen was not detected in normal peripheral blood, but was found in less than 1% of normal marrow cells. RTF8X did not inhibit in vitro colony formation of CFU-E and BFU-E in a complement-dependent cytotoxicity assay. Cell- sorting analysis showed that, morphologically, the RTF8X-positive marrow cells from the patients and normal volunteers contained more than 60% erythroblasts and that CFU-E and BFU-E were not demonstrated in cells with RTF8X antigen. Enzyme treatment suggested that RTF8X antigen was a sialoglycolipid. These results indicate that RTF8X may recognize the surface antigen found increasingly in association with tumors of erythroid lineage. RTF8X should be useful for studies of erythroid differentiation and proliferation in patients.

scholarly journals Precise Modeling of the Protective Effects of Quercetin against Mycotoxin via System Identification with Neural Networks

2019 ◽  
Vol 20 (7) ◽  
pp. 1725
Author(s):  
Changju Yang ◽  
Entaz Bahar ◽  
Shyam Adhikari ◽  
Seo-Jeong Kim ◽  
Hyongsuk Kim ◽  
...  
Keyword(s):  
Neural Networks ◽  
Artificial Neural Networks ◽  
Protective Effect ◽  
Cell Viability ◽  
Cell Lines ◽  
Cell Cytotoxicity ◽  
Hep G2 ◽  
Ldh Activity ◽  
Cytotoxicity Studies ◽  
Artificial Neural

Cell cytotoxicity assays, such as cell viability and lactate dehydrogenase (LDH) activity assays, play an important role in toxicological studies of pharmaceutical compounds. However, precise modeling for cytotoxicity studies is essential for successful drug discovery. The aim of our study was to develop a computational modeling that is capable of performing precise prediction, processing, and data representation of cell cytotoxicity. For this, we investigated protective effect of quercetin against various mycotoxins (MTXs), including citrinin (CTN), patulin (PAT), and zearalenol (ZEAR) in four different human cancer cell lines (HeLa, PC-3, Hep G2, and SK-N-MC) in vitro. In addition, the protective effect of quercetin (QCT) against various MTXs was verified via modeling of their nonlinear protective functions using artificial neural networks. The protective model of QCT is built precisely via learning of sparsely measured experimental data by the artificial neural networks (ANNs). The neuromodel revealed that QCT pretreatment at doses of 7.5 to 20 μg/mL significantly attenuated MTX-induced alteration of the cell viability and the LDH activity on HeLa, PC-3, Hep G2, and SK-N-MC cell lines. It has shown that the neuromodel can be used to predict the protective effect of QCT against MTX-induced cytotoxicity for the measurement of percentage (%) of inhibition, cell viability, and LDH activity of MTXs.

scholarly journals Oligo-2',5'-adenylate Synthetase Activity in K562 Cell Lines Persistently Infected with Measles or Mumps Virus

1988 ◽  
Vol 69 (8) ◽  
pp. 2085-2091 ◽  
Author(s):  
N. Fujii ◽  
K. Oguma ◽  
K. Kimura ◽  
T. Yamashita ◽  
S. Ishida ◽  
...  
Keyword(s):  
Cell Lines ◽  
K562 Cell ◽  
Mumps Virus ◽  
Synthetase Activity ◽  
Persistently Infected

scholarly journals Rubella Virus-Induced Apoptosis Varies among Cell Lines and Is Modulated by Bcl-XLand Caspase Inhibitors

Virology ◽  
1999 ◽  
Vol 255 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Robert Duncan ◽  
Jacqueline Muller ◽  
Nancy Lee ◽  
Ali Esmaili ◽  
Hira L. Nakhasi
Keyword(s):  
Cell Lines ◽  
Rubella Virus ◽  
Caspase Inhibitors ◽  
Induced Apoptosis

The interaction of 4-thiazolidinone derivatives containing indolin-2-one moiety with P-glycoprotein studied using K562 cell lines

2015 ◽  
Vol 101 ◽  
pp. 126-132 ◽  
Author(s):  
Feng Wang ◽  
Zijian Liu ◽  
Jian Wang ◽  
Jun Tao ◽  
Ping Gong ◽  
...  
Keyword(s):  
Cell Lines ◽  
K562 Cell ◽  
P Glycoprotein

scholarly journals Anti-K562 cell monoclonal antibody RTF8X recognizes tumor-associated antigen of erythroid lineage

Blood ◽  
1988 ◽  
Vol 72 (5) ◽  
pp. 1487-1491
Author(s):  
T Sakurai ◽  
H Hara ◽  
K Nagai
Keyword(s):  
Monoclonal Antibody ◽  
Cell Lines ◽  
Surface Antigen ◽  
K562 Cell ◽  
K562 Cells ◽  
Erythroid Differentiation ◽  
Enzyme Treatment ◽  
Normal Peripheral Blood ◽  
Marrow Cells

A new anti-K562 cell monoclonal antibody, RTF8X, a cytotoxic IgM, recognized a surface antigen on erythroblasts from patients with erythroleukemia and polycythemia vera. RTF8X, which is highly specific to K562 cells, did not react with the other 14 hematopoietic cell lines and the seven nonhematopoietic cell lines. RTF8X antigen was not detected in normal peripheral blood, but was found in less than 1% of normal marrow cells. RTF8X did not inhibit in vitro colony formation of CFU-E and BFU-E in a complement-dependent cytotoxicity assay. Cell- sorting analysis showed that, morphologically, the RTF8X-positive marrow cells from the patients and normal volunteers contained more than 60% erythroblasts and that CFU-E and BFU-E were not demonstrated in cells with RTF8X antigen. Enzyme treatment suggested that RTF8X antigen was a sialoglycolipid. These results indicate that RTF8X may recognize the surface antigen found increasingly in association with tumors of erythroid lineage. RTF8X should be useful for studies of erythroid differentiation and proliferation in patients.

Sign in / Sign up

Export Citation Format

Share Document