scholarly journals Cytotoxicity and antiproliferative effects of ethyl acetate fraction of Padina australis against MCM-B2 and K562 cell lines

2019 ◽  
Vol 7 (2) ◽  
pp. 25-29
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
K562 Cell ◽  
Ethyl Acetate Fraction ◽  
Antiproliferative Effects

scholarly journals Cytotoxic Alkaloids from Leaves and Twigs of Dasymaschalon sootepense

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Sakchai Hongthong ◽  
Chutima Kuhakarn ◽  
Vichai Reutrakul ◽  
Surawat Jariyawat ◽  
Pawinee Piyachaturawat ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Cancer Cell Lines ◽  
Spectroscopic Methods ◽  
Aporphine Alkaloid ◽  
First Report ◽  
Bioassay Guided Fractionation

Bioassay-guided fractionation of the cytotoxic ethyl acetate fraction of the sequential methanol extract from the leaves and twigs of Dasymaschalon sootepense led to the isolation of a new 7-hydroxy aporphine alkaloid, 6a,7-dehydrodasymachaline (1) along with the five known compounds (-)-nordicentrine (2), dicentrinone (3), (-)-sinactine (4), aristolactam AII (5) and epiberberine (6). Their structures were elucidated by spectroscopic methods. This is the first report of alkaloids 1–2 and 5–6 from the genus Dasymaschalon. Compounds 1 and 5 showed cytotoxicity against a panel of cancer cell lines.

In-Vitro Cytotoxic and Apoptotic Effect of Wrightia tinctoria Roxb. Alcoholic Extract/Fractions

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Charanjeet H Singh ◽  
Dharmendra Ahuja ◽  
S C Mehta
Keyword(s):  
Cell Cycle ◽  
Ethyl Acetate ◽  
Dna Fragmentation ◽  
Cell Lines ◽  
Fluorescent Microscopy ◽  
Ethyl Acetate Fraction ◽  
Alcoholic Extract ◽  
Malignant Growth ◽  
Hela Cell Lines ◽  
Wrightia Tinctoria

The ayurvedic medicine system of prescription suggests Wrightia tinctoria Roxb. (Apocynaceae), for the treatment of tumors. The investigation is required as malignant growth is the second most basic reason for death on the planet; the greater part of the customary medications have appeared, minimal effort approaches, and higher harmfulness of the current drugs. The present examination means to decide the method of cell death instigated by the alcoholic extract fractions of Wrightia tinctoria on human malignant growth cell lines. Amongst crude alcoholic extract of Wrightia tinctoria Roxb. investigate for potential anticancer action, there are two fractions selected for investigation these are WTD (dichloromethane fraction) and WTE (ethyl acetate fraction) hey were found to have capable cytotoxic potential on all cancerous cell lines contemplated by MTT test and SRB test. Ethyl acetate fraction found to have increasingly powerful action in A549, U343HeLa, BT549 and HCT15 cells lines. Strangely, ethyl acetate was less cytotoxic in HBL-100 cell line, demonstrate the particular action towards malignant growth cells. AO/EB staining assay and Hoechst-33342 staining assay demonstrate membrane blebbing, consolidated and fragmented nuclei upon treatment with ethyl acetate and dichloromethane fraction in A549, U343 and HeLa cell lines. DNA fragmentation stepping stool and genomic DNA discontinuity were seen with DNA fragmentation test dependent on gel electrophoresis and COMET test by fluorescent microscopy method. Essentially, cell cycle examination by flow cytometer demonstrates mutilation of ordinary cell cycle and expanded subG0phase. Ethyl acetate fraction was found genotoxic by micronuclei development. These outcomes show that both fractions instigate apoptosis however not cells decomposition in the malignant growth cells. This plant keeps potent anticancer activity in vivo and further researches are to recognize the responsible phytoconstituents and mechanism of action.

Cytotoxic Evaluation of Daphne pontica L. Aerial Part Extracts on Three Cancerous Cell Lines by MTT Assay

2019 ◽  
Author(s):  
Behshad Eskandari ◽  
Maliheh Safavi ◽  
Seyede Nargess Sadati Lamardi ◽  
Mahdi Vazirian
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Ethyl Acetate Fraction ◽  
Chemotherapeutic Agents ◽  
Breast Cancer Cell Lines ◽  
Total Extract ◽  
Cancerous Cell

Nowadays, cancer is the second prevalent cause of mortality after cardiovascular diseases in developed and the third one in developing countries. Adverse effects of chemotherapeutic agents bring the necessity of investigating about new medications with fewer side effects. Daphne L. genus is one of the natural sources with valuable reported anticancer effects. This study aimed to assess the cytotoxic effect of some extracts from the aerial parts of Daphne pontica collected from North of Iran on cancer cell lines.Extraction of the plant material was performed by maceration (3×72 h) of 200 g of sample with petroleum ether, chloroform, ethyl acetate, and methanol, respectively. The total extract was also obtained by maceration of the sample with 80% ethanol. Different concentrations of the dried extracts were prepared to assess their cytotoxic effect by 24 h incubation of cell lines with different extracts and then MTT (dimethyl thiazolyl diphenyl tetrazolium) assay on three cancerous cell lines (MDA-MB-231, MCF-7 and T47D), performed in triplicate. IC50 was then estimated from curves constructed by plotting cell survival (%) versus sample concentration (µg/ml). Results indicated that ethyl acetate fraction of D. pontica had the most potent cytotoxic effect in MTT assay with IC50 = 977.46 µg/ml; while other fractions were weaker in toxicity (IC50>1000 µg/ml). By comparing to potent cytotoxic effects of other Daphne species, it seems that the cytotoxic properties of D. pontica is different from other species of this genus since according to this study, no significant antineoplastic properties against the three breast cancer cell lines were determined. Further studies on other pharmacological activities of this plant are recommended.

Effect of Ethyl Acetate Fraction of Marsilea crenata Presl. Leaf Extract on Major Histocompatibility Complex Class II Expression in Microglial HMC3 Cell Lines

2021 ◽  
pp. 6374-6378
Author(s):  
Mangestuti Agil ◽  
Hening Laswati ◽  
Hadi Kuncoro ◽  
Burhan Ma’arif
Keyword(s):  
Major Histocompatibility Complex ◽  
Ethyl Acetate ◽  
Major Histocompatibility Complex Class ◽  
Cell Lines ◽  
Leaf Extract ◽  
Ethyl Acetate Fraction ◽  
Complex Class ◽  
Mhc Ii ◽  
Histocompatibility Complex ◽  
M1 Phenotype

Phytoestrogens are plant-derived chemical substances that have estrogen-like structures or estrogenic functions. Deficiency of estrogen in human brain causes neuroinflammation characterized by increase of major histocompatibility complex class II (MHC II) expression as a marker of M1 phenotype in microglia. Recent research found phytoestrogen compounds in Marsilea crenata Presl. The aim of this study was to investigate the effect of ethyl acetate fraction of Marsilea crenata Presl. leaf extract in MHC II expression of microglial HMC3 cell lines, for resolution of inflammation and tissue repair. The fractions were given at concentrations of 62.5, 125, and 250 ppm to microglia, that had been previously induced by IFNγ 10 ng for 24 hours to stimulate the cells into M1 phenotype. Genistein as phytoestrogen was given at a concentration of 50 μM as positive control. Expression of MHC II was analyzed using immunocytochemistry method. Result showed reduction in MHC II expression of microglial cells, which indicated the activity of all extracts and, showed that 250 ppm of the fraction showed the strongest effect with MHC II value expression of 148.632 AU, and ED50 of 1,590 ppm. It was concluded from the study, that ethyl acetate fraction of Marsilea crenata Presl. leaves has antineuroinflammation effect.

Notes: Flavonols from Scurrula ferruginea Danser (Loranthaceae)

2002 ◽  
Vol 57 (11-12) ◽  
pp. 1092-1096 ◽  
Author(s):  
Françoise Lohézic-Le Dévéhat ◽  
Sophie Tomasi ◽  
Didier Fontanel ◽  
Joël Boustie
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Ethyl Acetate Fraction ◽  
Flavonol Glycoside ◽  
Spectroscopic Methods ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Isolated Structures

Three natural flavonols compounds have been isolated from the ethyl acetate fraction of Scurrula ferruginea Danser (Loranthaceae). Besides quercetin and quercitrin, an unusual flavonol glycoside 4″-O-acetylquercitrin was isolated. Structures were determined using spectroscopic methods including UV, NMR and HRMS-EI. The incidence of 4″-O-acetylquercitrin, not previously reported in the Loranthaceae, is discussed. Cytotoxic evaluation on four human cancer cell lines showed quercetin to be the most active with IC50 of 35 μм on U251 (human glioblastoma cells).

scholarly journals Leea indicaEthyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Wong Yau Hsiung ◽  
Habsah Abdul Kadir
Keyword(s):  
Cervical Cancer ◽  
Ethyl Acetate ◽  
Cancer Cells ◽  
Cell Lines ◽  
Morphological Changes ◽  
Chromatin Condensation ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Cervical Cancer Cells ◽  
Leea Indica

The anticancer potential ofLeea indica, a Chinese medicinal plant was investigated for the first time. The crude ethanol extract and fractions (ethyl acetate, hexane, and water) ofLeea indicawere evaluated their cytotoxicity on various cell lines (Ca Ski, MCF 7, MDA-MB-435, KB, HEP G2, WRL 68, and Vero) by MTT assay.Leea indicaethyl acetate fraction (LIEAF) was found showing the greatest cytotoxic effect against Ca Ski cervical cancer cells. Typical apoptotic morphological changes such as DNA fragmentation and chromatin condensation were observed in LIEAF-treated cells. Early signs of apoptosis such as externalization of phosphatidylserine and disruption of mitochondrial membrane potential indicated apoptosis induction. This was further substantiated by dose- and time-dependent accumulation of sub-G1cells, depletion of intracellular glutathione, and activation of caspase-3. In conclusion, these results suggested that LIEAF inhibited cervical cancer cells growth by inducing apoptosis and could be developed as potential anticancer drugs.

scholarly journals Cytotoxic Effects of Compounds Isolated from Ricinodendron heudelotii

Molecules ◽  
2019 ◽  
Vol 24 (1) ◽  
pp. 145 ◽  
Author(s):  
Omolara F. Yakubu ◽  
Abiodun H. Adebayo ◽  
Titilope M. Dokunmu ◽  
Ying-Jun Zhang ◽  
Emeka E.J. Iweala
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Fraction ◽  
Cytotoxic Effects ◽  
Ic50 Value ◽  
Ricinodendron Heudelotii ◽  
Galloyl Glucose ◽  
Mcf 7

This study was designed to explore the in vitro anticancer effects of the bioactive compounds isolated from Ricinodendron heudelotii on selected cancer cell lines. The leaves of the plant were extracted with ethanol and partitioned in sequence with petroleum ether, ethyl acetate, and n-butanol. The ethyl acetate fraction was phytochemically studied using thin layer chromatography (TLC) and column chromatography (CC). Structural elucidation of pure compounds obtained from the ethyl acetate fraction was done using mass spectra, 1H-NMR, and 13C-NMR analysis. The isolated compounds were subsequently screened using five different cancer cell lines: HL-60, SMMC-7721, A-549, MCF-7, SW-480, and normal lung epithelial cell line, BEAS-2B, to assess their cytotoxic effects. Nine compounds were isolated and structurally elucidated as gallic acid, gallic acid ethyl ester, corilagin, quercetin-3-O-rhamnoside, myricetin-3-O-rhamnoside, 1,4,6-tri-O-galloyl glucose, 3,4,6-tri-O-galloyl glucose, 1,2,6-tri-O-galloyl glucose, and 4,6-di-O-galloyl glucose. Corilagin exhibited the most cytotoxic activity with an IC50 value of 33.18 μg/mL against MCF-7 cells, which were comparable to cisplatin with an IC50 value of 27.43 µg/mL. The result suggests that corilagin isolated from R. heudelotii has the potential to be developed as an effective therapeutic agent against the growth of breast cancer cells.

Antiproliferative activity of longan (Dimocarpus longan Lour.) leaf extracts

2020 ◽  
Author(s):  
Ratna Puspita ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Vero Cells ◽  
Leaf Extracts ◽  
Water Fraction ◽  
Lc50 Value

Longan (Dimocarpus longan Lour.) belongs to the Sapindaceae family. We examined the antiproliferative activity of longan leaf extracts against cancer-derived cell lines in vitro. The tested samples were water extract, ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction of longan leaf. Cytotoxicity test is against brine shrimps that were screened using Brine Shrimp Lethality Test. Antiproliferative activity assay on WEHI-164 cells (mouse fibrosarcoma cancer cell), THP-1 cells (human peripheral blood acute monocyte cell), and Vero cells (non-cancer or normal cell) that was conducted using a hemocytometer with Trypan Blue Dye exclusion. The 50% lethality concentration (LC50) value of water extract, ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 854.64, 305.81, 446.55, 1313.44, and 1621.8 μg/ml. Ethanol extract exhibited significant cytotoxic due to the lowest LC50 value. The ethanol extract was then used for further examination. The highest antiproliferative activity was achieved 44.93% by 600 μg/ml ethanol extract on WEHI-164 and 57.45% by 500 μg/ml ethanol extract on THP-1. It was significantly equal to doxorubicin antiproliferative activity. Ethanol extract dose had a low effect on Vero cells. This present study confirmed that the longan leaf ethanol extract possesses marked antiproliferative activity on cancer-derived cell lines.

scholarly journals ANTIOXIDANT AND ANTI-PROLIFERATIVE EFFECTS OF AN ETHYL ACETATE FRACTION OF THE HYDRO-ETHANOLIC EXTRACT OF SYNEDRELLA NODIFLORA (L) GAERTN

2020 ◽  
pp. 136-143
Author(s):  
PATRICK AMOATENG ◽  
EMELIA OPPONG BEKOE ◽  
SETH KWABENA AMPONSAH ◽  
REGINA APPIAH-OPONG ◽  
DORCAS OSEI-SAFO ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Normal Liver ◽  
Ethyl Acetate Fraction ◽  
Liver Cells ◽  
Total Phenol ◽  
Synedrella Nodiflora ◽  
Mcf 7

Objective: Synedrella nodiflora is traditionally used in the treatment of several ailments. Pharmacologically, this plant has anticonvulsant, sedative, anti-nociceptive and anti-proliferative effects. This study further investigated S. nodiflora for its antioxidant and in vitro inhibition of cancerous cell lines. Methods: Phytochemical assays, and the DPPH radical scavenging method were employed in preliminary screening for antioxidant activities of the crude hydro-ethanolic extract (SNE) and resulting fractions. The potent ethyl acetate fraction (EAF), was further investigated for total phenol and flavonoid contents, reducing power, lipid peroxidation potential, and cytotoxic effects on human breast cancer (MCF-7), leukemic (Jurkat), and normal liver (Chang’s liver) cell lines. Results: The extract contained phenols, flavonoids, tannins, glycosides, sterols, terpenoids, and alkaloids. It scavenged for DPPH with an IC50 of 114 µg/ml, whereas that of EAF was 8.9 µg/ml. EAF prevented peroxidation of egg lecithin at an IC50 of 24.01±0.08 µg/ml. These IC50s are four and three times lower than the reference standards. EAF produced anti-proliferative effects against MCF-7, and Jurkat cell lines with IC50s of 205.2 and 170.9 µg/ml, respectively. EAF had a high IC50 of 252.2 µg/ml against Chang’s liver cells. At 0.1 mg/ml EAF had similar total flavonoid content to SNE, but a significantly higher total phenol content. Conclusion: The ethyl acetate fraction of S. nodiflora, exhibited the most potent antioxidant activity. It inhibited the proliferation of breast and leukemic cancer cell lines, whiles having weak cytotoxic effect on normal liver cells. These can be explored for further drug development.

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