Effect of melatonin treatment on semen parameters and endocrine function in Black Racka rams out of the breeding season

2014 ◽  
Vol 116 (2-3) ◽  
pp. 192-198 ◽  
Author(s):  
I. Egerszegi ◽  
P. Sarlós ◽  
J. Rátky ◽  
L. Solti ◽  
V. Faigl ◽  
...  
Keyword(s):  
Breeding Season ◽  
Endocrine Function ◽  
Semen Parameters ◽  
Melatonin Treatment

Testicular function and semen characteristics of Awassi rams treated with melatonin out of the breeding season

2009 ◽  
Vol 57 (4) ◽  
pp. 531-540 ◽  
Author(s):  
Vera Faigl ◽  
Mónika Keresztes ◽  
Margit Kulcsár ◽  
Sándor Nagy ◽  
Zsuzsanna Keresztes ◽  
...  
Keyword(s):  
Breeding Season ◽  
Slow Release ◽  
Endocrine Function ◽  
Semen Parameters ◽  
Control Group ◽  
Melatonin Treatment ◽  
Semen Characteristics ◽  
Igf I ◽  
Semen Donor

The objective of this study was to evaluate the effect of long-term melatonin treatment applied during the non-breeding season on semen characteristics, endocrine function of testicles and baseline level of insulin-like growth factor-I (IGF-I) in Awassi rams kept in the temperate continental zone of Europe and used as semen donors in an artificial insemination (AI) programme. On 23 February (day 0), slow-release melatonin implants were inserted subcutaneously into rams (n = 8). Control animals (n = 8) received no treatment. In both groups, basic semen parameters (concentration, total motility, fast and slow forward motility, morphology), GnRH-induced testosterone response and basal IGF-I concentration were evaluated on days 0, 47 and 71. No differences were found in concentration of spermatozoa, total motility, and numbers of spermatozoa with fast and slow progressive motility and normal/abnormal morphology between the melatonin-treated and the control group. However, in melatonin-treated animals, basal and GnRH-induced testosterone levels were slightly elevated on day 47 and became significantly higher on day 71 (P < 0.05) as compared to controls. There was no difference in plasma IGF-I levels between the groups. In conclusion, slow-release melatonin applied during the non-breeding season improves testicular testosterone production but does not influence the semen characteristics and the IGF-I level of semen donor Awassi rams used in an AI programme and kept in the temperate continental zone of Europe.

The effect of melatonin treatment during the seasonal anoestrus on the superovulatory response and embryo production of high-prolificacy Rasa Aragonesa ewes before culling

2003 ◽  
Vol 2003 ◽  
pp. 82-82
Author(s):  
F. Forcada ◽  
J.A. Abecia ◽  
J.A. Valares
Keyword(s):  
Embryonic Development ◽  
Breeding Season ◽  
Litter Size ◽  
Direct Effect ◽  
Ovulation Rate ◽  
Corpora Lutea ◽  
Melatonin Treatment ◽  
Embryo Production ◽  
Spring Equinox

The efficacy of melatonin implants inserted around the spring equinox to improve fertility and ovulation rate or litter size in Mediterranean ewes has been previously reported (Chemineau et al., 1996; Forcada et al., 2002a.), indicating the ability of the hormone to regulate the hypothalamic activity (Viguié et al., 1995). Moreover, a direct effect of melatonin on corpora lutea and embryonic development has been also reported (Wallace et al., 1988; Abecia et al., 2002). The use of prolific Rasa Aragonesa (RA) ewes (a Mediterranean breed) before culling as embryo donors has been previously tested in the breeding season (Forcada et al., 2002b.). The aim of this experiment was to improve embryo production during the seasonal anoestrus period in selected superovulated RA ewes at the end of their reproductive lives through the use of melatonin.

110 EFFECT OF MELATONIN TREATMENT ON LIBIDO AND ENDOCRINE FUNCTION OF DROMEDARY CAMEL BULLS OUT OF THE BREEDING SEASON

2017 ◽  
Vol 29 (1) ◽  
pp. 163 ◽  
Author(s):  
A. Swelum ◽  
I. Saadeldin ◽  
H. Ba-Awadh ◽  
A. Alowaimer
Keyword(s):  
Breeding Season ◽  
Reproductive Performance ◽  
Repeated Measures ◽  
Endocrine Function ◽  
Gland Secretion ◽  
Control Group ◽  
Dromedary Camel ◽  
Testosterone Levels ◽  
Significant Difference ◽  
Length Changes

The reproductive performance of camels is poor and has remained a major obstacle to the growth of dromedary populations. The limited breeding season is one of the most important causes of the poor reproductive performance. In seasonal animals, melatonin is the chemical messenger that allows the perception of daylight length changes. Commercial melatonin products have been developed for the manipulation of seasonal breeding in animals. The present study aimed to evaluate the influence of melatonin implantation on libido, serum melatonin, and testosterone concentrations in dromedary camels during the non-breeding season (June and July). Ten camel bulls were used in the 35-day-long trial; 5 of them were implanted with 30 Melovine® implant (Ceva, Libourne, France) subcutaneously on Day 0, whereas the other 5 camel bulls remained untreated as a control. Libido was evaluated weekly in response to oestrous-induced female camels treated with oestrogen (1 mL Oestrocon; oestradiol benzoate 5 mg mL−1) 2 days before assessment of libido. Libido was scored as follows: 0 = not interested: the male did not show any libido; 1 = low interested: the male went near the female and showed low frequency of sniffing and flehmen; 2 = interested: the male went near the female, it showed sniffing, flehmen, grinding of teeth/whistling, yawning; 3 = high interested: the male went near the female and was very agitated, it showed sniffing, flehmen, grinding of teeth/whistling, yawning, urination, and tail raising. It stood with open legs, and poll gland secretion and neck rubbing were observed; 4 = excited, like 3, but the male showed blatering and dulaa extrusion, was very excited, stood with open legs, high poll gland secretion and neck rubbing were observed. Blood samples were collected weekly. Serum melatonin and testosterone concentrations were evaluated using commercial ELISA kits. Comparisons among groups were evaluated using repeated-measures ANOVA, using SAS software (SAS Institute Inc., Cary, NC, USA). A difference was considered significant at the P < 0.05 level. The results revealed that at Day 0, all camel bulls in 2 groups had no libido and there was no significant difference in the melatonin or testosterone levels in the 2 groups. The libido increased gradually in the melatonin group and reached the maximum (3–5) at week 4 and week 5. The control group had low libido (0–1) along the trial. Statistically, the libido was significantly higher in the melatonin group than control group. Additionally, testosterone levels were significantly higher in melatonin group than control group, especially in the fourth week of the present trial (565.07 ± 33.04 pg mL−1 and 458.49 ± 25.36 pg mL−1, respectively). In conclusion, melatonin implantation in the non-breeding season significantly improved the libido and the reproductive performance of dromedary camel bulls. Therefore, it may be possible to improve the reproductive efficiency of camels by extending the breeding season through treatment with melatonin during the non-breeding season.

scholarly journals Effects of coadministration of DHA and vitamin E on spermatogram, seminal oxidative stress, and sperm phospholipids in asthenozoospermic men: a randomized controlled trial

2020 ◽  
Vol 112 (3) ◽  
pp. 707-719 ◽  
Author(s):  
Ghazaleh Eslamian ◽  
Naser Amirjannati ◽  
Nazanin Noori ◽  
Mohammad-Reza Sadeghi ◽  
Azita Hekmatdoost
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Sperm Count ◽  
Controlled Trial ◽  
Sperm Concentration ◽  
Endocrine Function ◽  
Semen Parameters ◽  
Double Blind

ABSTRACT Background It is unknown which compounds in spermatozoa or seminal plasma may be involved in the regulation of sperm motility. Objectives The aim of this study was to investigate the effects of DHA (22:6n–3), vitamin E, and their probable interactions in men with asthenozoospermia. Methods A factorial, randomized, double-blind, placebo-controlled trial was conducted in infertility clinics in Tehran, Iran. The participants were idiopathic asthenozoospermic men aged 20–45 y, with normal endocrine function. Their concentration of spermatozoa and percentage of morphologically normal spermatozoa were equal to or above the lower reference limits, according to the fifth edition of the WHO guideline. Out of 717 men referred to the infertility clinics, 180 asthenozoospermic men were randomly assigned to 1 of 4 groups according to stratified blocked randomization by age and sperm concentration. Participants took daily 465 mg DHA plus 600 IU vitamin E (DE), 465 mg DHA plus placebo (DP), 600 IU vitamin E plus placebo (EP), or both placebo capsules (PP) for 12 wk. Sperm characteristics, oxidative stress of seminal plasma, serum and sperm membrane fatty acids, dietary intakes, anthropometric measurements, and physical activity were measured at baseline and after 12 wk. Results After the intervention, mean ± SD sperm progressive motility was greater in the DE group (27.9 ± 2.8) than in the DP (25.7 ± 3.4), EP (26.1 ± 2.8), and PP (25.8 ± 2.6) groups (P &lt; 0.05). Sperm count (P = 0.001) and concentration (P = 0.044) increased significantly in the DE group compared with the other 3 groups, whereas other semen parameters were not significantly different between the groups after the intervention. Serum concentrations of n–3 PUFAs were significantly higher in the DE and DP groups than in the EP and PP groups. Conclusions Combined DHA and vitamin E supplements led to increased sperm motility; however, no significant changes occurred in sperm morphology and vitality in asthenozoospermic men. This trial was registered at clinicaltrials.gov as NCT01846325.

Effect of melatonin on the peripheral concentrations of LH and progesterone after oestrus, and on conception rate in ewes

1988 ◽  
Vol 119 (3) ◽  
pp. 523-530 ◽  
Author(s):  
J. M. Wallace ◽  
J. J. Robinson ◽  
S. Wigzell ◽  
R. P. Aitken
Keyword(s):  
Breeding Season ◽  
Pulse Generator ◽  
Ovulation Rate ◽  
Melatonin Treatment ◽  
Embryo Survival ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Endocrine Status ◽  
Increased Activity ◽  
Intra Uterine Insemination

ABSTRACT It has previously been shown that administration of the indoleamine melatonin to advance the breeding season of ewes is also associated with an increase in ovulation rate and subsequent litter size. Experiment 1 assessed whether, in ewes receiving melatonin to advance the breeding season, the indoleamine acts directly on the corpus luteum to enhance progesterone secretion or indirectly through increased activity of the hypothalamic pulse generator. Six ewes received 3 mg melatonin orally at 15.00 h daily from 22 March onwards, six were induced to ovulate during mid-anoestrus following withdrawal of a progestagen pessary and injection of exogenous gonadotrophin and six acted as naturally ovulating controls. First overt oestrus occurred between 17 May and 8 July in melatonin-treated ewes, between 21 October and 3 January in control ewes and on 8 July in all induced ewes. On days 2 and 10 after the first overt oestrus, melatonin-treated ewes had pulsatile LH activity characteristic of that measured in control ewes ovulating naturally during the breeding season. There was an absence of any pulsatile LH activity in the induced ewes. Progesterone concentrations between days 7 and 12 following oestrus were significantly higher in melatonin-treated than in control and induced ewes, suggesting a luteotrophic role for melatonin. Experiment 2 was carried out to determine whether administration of melatonin commencing after induced ovulation and insemination would alter the endocrine status of the ewe and thereby influence the establishment of pregnancy and embryo survival. Thirty-two anoestrous ewes were induced to ovulate on 29 June. Starting 24 h after intra-uterine insemination, 16 ewes were given melatonin daily for 60 days and 16 acted as controls. Daily LH concentrations were higher in melatonin-treated than in control ewes from days 2 to 22 after oestrus, while prolactin concentrations declined in melatonin-treated ewes over the same period. Plasma progesterone concentrations were enhanced in melatonin-treated ewes between days 4 and 9 following oestrus, yet ovulation rates were the same as for controls. Successful pregnancies occurred in 0·56 control (9 of 16) and 0·69 melatonin-treated (11 of 16) ewes. For these ewes the number of fetuses surviving to term as a proportion of ovulation rate was 0·43 and 0·51 for the control and melatonin treatment respectively. J. Endocr. (1988) 119, 523–530

scholarly journals P–097 The impact of SARS-CoV–2 on male gonadal function. A longitudinal study

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
M P Lauritsen ◽  
T D Leineweber ◽  
C B Hansen ◽  
U V Schneider ◽  
H Westh ◽  
...  
Keyword(s):  
Cohort Study ◽  
Semen Quality ◽  
Endocrine Function ◽  
Semen Parameters ◽  
Reproductive Hormone ◽  
Rt Pcr ◽  
Hormone Profile ◽  
Oropharyngeal Swab ◽  
The Impact

Abstract Study question Can severe acute respiratory syndrome coronavirus 2 (SARS-CoV–2) be detected in the semen of SARS-CoV–2 positive men, and does SARS-CoV–2 infection affect male reproductive function? Summary answer No SARS-CoV–2 RNA was detected in semen. An impact of SARS-CoV–2 infection on semen quality and reproductive hormone profile awaits evaluation at 3 + 6 months follow-up. What is known already SARS-CoV–2 may use angiotensin-converting enzyme (ACE)2 as an entry point into the cell. As ACE2 is expressed in testicular tissue, it has been speculated that SARS-CoV–2 may affect the male reproductive system. A cohort study including 38 male COVID–19 patients showed that SARS-CoV–2 was present in the semen of six patients (15.8%) [Li et al., 2020]. Later studies including a total of 223 patients have not provided evidence of transmission of SARS-CoV–2 via semen. There are to date no available longitudinal studies on semen quality following SARS-CoV–2 infection. Study design, size, duration Longitudinal cohort study including 50 non-hospitalized men from the general population in the Capital Region of Denmark. All participants had a confirmed SARS-CoV–2 infection by reverse-transcription polymerase chain reaction (RT-PCR) on oropharyngeal swab material within the last week. The presence of SARS-CoV–2 in semen samples by RT-PCR, semen parameters and reproductive hormone profile were assessed at inclusion and at 3 + 6 months follow-up. SARS-CoV–2 antibody levels were assessed 3–5 weeks after inclusion. Participants/materials, setting, methods SARS-CoV–2-positive males (age 18–60 years) were included. Oropharyngeal and semen samples were tested by RT-PCR applying the E-Sarbeco primers and probe published by Corman et al. 2020 and adapted to TaqMan Fast Virus 1-step master mix and LightCycler 480 as previously reported by Jørgensen et al. 2020. SARS-CoV–2 antibodies were detected using the serological immunoassay from Shenzhen YHLO Biotech on the iFlash 1800. Semen quality parameters were analysed according to World Health Organisation (WHO) standards. Main results and the role of chance To date, 25 men with a mean age of 35 years have been included in the study. SARS-CoV–2 RNA could not be detected in the semen samples of any of the 25 men at the time of inclusion. Twenty-one of the 25 men (84,0%) had a same day RT-PCR-confirmed SARS-CoV–2 infection in an oropharyngeal swab. RT-PCR cycle threshold (ct) values were distributed as follows: four (19,0%) were strongly positive (ct &lt; 25), 16 (76,2%) intermediately positive (ct 25–35) and one (4,8%) weakly positive (ct 35–45). The four men without PCR-confirmed SARS-CoV–2 infection all had a positive IgG response to SARS-CoV–2 at the time of inclusion. Longitudinal semen and reproductive hormone profiles analyses will be performed. Further studies are needed to prove whether SARS-CoV–2 can be transmitted to the male reproductive tract and whether SARS-CoV–2 infection may cause alterations of spermatogenesis and endocrine function. Limitations, reasons for caution Strengths of this study are the unselected population of men examined within a week after confirmed SARS-CoV–2 infection and the follow-up of semen parameters and endocrine profile. Limitations are the limited sample size and the fact that semen quality was not known before the participants were diagnosed with COVID–19. Wider implications of the findings: Knowledge of viral detection and semen persistence of SARS-CoV–2 is essential for clinical practice and public health. There is a need for evidence-based counselling on the impact of SARS-CoV–2 infection for patients undergoing assisted reproduction technology and patients who have a need for semen cryopreservation. Trial registration number H–20027362

Duration of melatonin treatment and ovarian activity in ewes

1988 ◽  
Vol 1988 ◽  
pp. 92-92
Author(s):  
S. Wigzell ◽  
J.J. Robinson ◽  
J.M. Wallace ◽  
R.P. Aitken
Keyword(s):  
Breeding Season ◽  
Ovarian Activity ◽  
Similar Reaction ◽  
Melatonin Treatment ◽  
Oral Dosing ◽  
Starting Time ◽  
Highly Effective ◽  
Natural Photoperiod ◽  
The Mean

Daily oral dosing in mid afternoon with 3 mg of the indoleamine, melatonin, from early June onwards is highly effective in advancing the breeding season of both Greyface and Scottish Blackface ewes kept under a natural photoperiod; the mean reaction interval from initial dosing to the onset of ovarian activity in Scottish Blackface ewes being around 65 days compared with 110 days for untreated controls (Robinson et al 1985). Advancing the starting time for the melatonin treatment to mid-March gave a similar reaction interval with behavioural oestrus occurring in late May/early June (Wigzell et al 1986a). Following this reversal of the breeding season by melatonin, continued melatonin treatment in unmated ewes resulted in a period of oestrous cyclicity lasting 4 to 5 months. Thereafter the ewes became refractory to melatonin and returned to anoestrus at a time when untreated controls were commencing ovarian activity (Wigzell et al 1986b). These observations prompted us to investigate the effects of different durations of melatonin treatment on ovarian activity.

Effects of melatonin implantation on the fertility potentials of Kivircik and Charollais ewes and rams during the non-breeding season

2017 ◽  
Vol 20 (3) ◽  
pp. 501-506 ◽  
Author(s):  
M. Cevik ◽  
C. Yilmazer ◽  
A. Kocyigit
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Reproductive Performance ◽  
Reproductive Potential ◽  
Testicular Volume ◽  
Melatonin Treatment ◽  
Scrotal Circumference ◽  
Estrus Response

AbstractThis study examined the effect of melatonin implantation during the non-breeding season on the reproductive performance of ewes and the testicular dimensions of rams. In seasonally anestrus Kivircik and Charollais ewes and rams were subjected to melatonin. Estrus response was significantly higher in treated than control ewes of both breeds (p<0.001). The pregnancy rate was significantly lower (p<0.001) in the control than in the treated animals. The twinning rate was significantly lower in melatonin implanted Kivircik than Charollais ewes (p<0.05). The testicular dimensions after 42 days of melatonin treatment increased in both breeds. Scrotal length (SL) increased in Kivircik and Charollais rams (p<0.01). The increase in scrotal circumference (SC) was more marked in the Charollais (P<0.01) than in the Kivircik rams. There was a large increase in testicular volume (TV) in both Kivircik (p<0.01) and Charollais (p<0.001) rams. This study shows that melatonin implants can be applied to induce estrus in ewes approximately four months earlier than breeding season. Melatonin implantation in the non-breeding season significantly increased testicular dimensions in Kivircik and Charollais rams thus increasing their reproductive potential.

Melatonin treatment prior to the normal breeding season increases fetal number in United Kingdom sheep flocks

2009 ◽  
Vol 182 (2) ◽  
pp. 198-202 ◽  
Author(s):  
P.R. Scott ◽  
N.D. Sargison ◽  
A.I. Macrae ◽  
M.R. Gough
Keyword(s):  
United Kingdom ◽  
Breeding Season ◽  
Melatonin Treatment
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