scholarly journals Effects of coadministration of DHA and vitamin E on spermatogram, seminal oxidative stress, and sperm phospholipids in asthenozoospermic men: a randomized controlled trial

2020 ◽  
Vol 112 (3) ◽  
pp. 707-719 ◽  
Author(s):  
Ghazaleh Eslamian ◽  
Naser Amirjannati ◽  
Nazanin Noori ◽  
Mohammad-Reza Sadeghi ◽  
Azita Hekmatdoost
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Sperm Count ◽  
Controlled Trial ◽  
Sperm Concentration ◽  
Endocrine Function ◽  
Semen Parameters ◽  
Double Blind

ABSTRACT Background It is unknown which compounds in spermatozoa or seminal plasma may be involved in the regulation of sperm motility. Objectives The aim of this study was to investigate the effects of DHA (22:6n–3), vitamin E, and their probable interactions in men with asthenozoospermia. Methods A factorial, randomized, double-blind, placebo-controlled trial was conducted in infertility clinics in Tehran, Iran. The participants were idiopathic asthenozoospermic men aged 20–45 y, with normal endocrine function. Their concentration of spermatozoa and percentage of morphologically normal spermatozoa were equal to or above the lower reference limits, according to the fifth edition of the WHO guideline. Out of 717 men referred to the infertility clinics, 180 asthenozoospermic men were randomly assigned to 1 of 4 groups according to stratified blocked randomization by age and sperm concentration. Participants took daily 465 mg DHA plus 600 IU vitamin E (DE), 465 mg DHA plus placebo (DP), 600 IU vitamin E plus placebo (EP), or both placebo capsules (PP) for 12 wk. Sperm characteristics, oxidative stress of seminal plasma, serum and sperm membrane fatty acids, dietary intakes, anthropometric measurements, and physical activity were measured at baseline and after 12 wk. Results After the intervention, mean ± SD sperm progressive motility was greater in the DE group (27.9 ± 2.8) than in the DP (25.7 ± 3.4), EP (26.1 ± 2.8), and PP (25.8 ± 2.6) groups (P < 0.05). Sperm count (P = 0.001) and concentration (P = 0.044) increased significantly in the DE group compared with the other 3 groups, whereas other semen parameters were not significantly different between the groups after the intervention. Serum concentrations of n–3 PUFAs were significantly higher in the DE and DP groups than in the EP and PP groups. Conclusions Combined DHA and vitamin E supplements led to increased sperm motility; however, no significant changes occurred in sperm morphology and vitality in asthenozoospermic men. This trial was registered at clinicaltrials.gov as NCT01846325.

scholarly journals Antioxidant cosupplementation therapy with vitamin C, vitamin E, and coenzyme Q10 in patients with oligoasthenozoospermia

2014 ◽  
Vol 86 (1) ◽  
pp. 1 ◽  
Author(s):  
Yoshitomo Kobori ◽  
Shigeyuki Ota ◽  
Ryo Sato ◽  
Hiroshi Yagi ◽  
Shigehiro Soh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Coenzyme Q10 ◽  
Cell Structure ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Coenzyme Q10 Supplementation ◽  
Therapy Treatment

Objective: Overproduction of reactive oxygen species results in oxidative stress, a deleterious process that damages cell structure as well as lipids, proteins, and DNA. Oxidative stress plays a major role in various human diseases, such as oligoasthenozoospermia syndrome. Materials and methods: We evaluated the effectiveness of antioxidant co-supplementation therapy using vitamin C, vitamin E, and coenzyme Q10 in men with oligoasthenozoospermia. Overall, 169 infertile men with oligoasthenozoospermia received antioxidant therapy with 80 mg/day vitamin C, 40 mg/day vitamin E, and 120 mg/day coenzyme Q10. We evaluated spermiogram parameters at baseline and at 3 and 6 months of follow-up. Results: Significant improvements were evident in sperm concentration and motility following coenzyme Q10 therapy. Treatment resulted in 48 (28.4%) partner pregnancies, of which 16 (9.5%) were spontaneous. Significant improvements in sperm cell concentration and sperm motility were observed after 3 and 6 months of treatment. Conclusions: Vitamin C, vitamin E, and coenzyme Q10 supplementation resulted in a significant improvement in certain semen parameters. However, further studies are needed to empirically determine the effect of supplementation on pregnancy rate.

scholarly journals How does seminal plasma fatty-acid binding protein-9 level change in infertile males?

2020 ◽  
Vol 107 (3) ◽  
pp. 419-430
Author(s):  
E. Menevse ◽  
R. Sevinc ◽  
D. Dursunoglu ◽  
N. Akdam ◽  
E.N. Korucu
Keyword(s):  
Fatty Acid ◽  
Seminal Plasma ◽  
Sperm Count ◽  
Binding Protein ◽  
Sperm Concentration ◽  
Lipid Binding ◽  
Semen Parameters ◽  
Fatty Acid Binding Proteins ◽  
Specific Expression ◽  
Fatty Acid Binding

AbstractIn recent years, free fatty acid binding proteins (FABPs) are implicated in spermatogenesis and sperm morphology. FABPs are members of the intracellular lipid-binding protein family; they exhibit tissue specific expression like the FABP9/PERF15 (Perforated15) male germ cell-specific fatty acid linkage-protein.The aim of the study was to assess the levels of seminal FABP-9 in normozoospermic and oligozoospermic men, and the possible relations between seminal FABP-9 levels and semen parameters.Research was carried out on 60 male volunteers who were admitted to Selcuk University Faculty of Medicine of Andrology Laboratory. Normozoospermic individuals (n = 30) were identified as Group 1, and Oligozoospermic individuals (n = 30) were identified as Group 2. The semen samples were collected in sterile plastic containers. Sperm parameters were assessed according to Kruger's criteria. Seminal plasma FABP-9 levels were analyzed by ELISA method. Outcomes were statistically evaluated at 0.05 significance level with SPSS (22.0). The Receiver Operating Characteristic (ROC) curve was used to evaluate the performance of FABP-9 levels as compared to that of the concentration and motility data of the sperm. FABP-9 levels were significantly higher in normozoospermic individuals (3.41 ± 1.64 ng/mL) than in oligozoospermic individuals (1.99 ± 0.78 ng/mL). There were significant correlations between FABP-9 levels and sperm concentration, total sperm count, motility, progressive motility, immobility, Total Progressive Motil Sperm Count (TPMSC), head anomaly, and teratozoospermia index.We suggest that FABP-9 level is an important biomarker, and low levels of semen FABP-9 may impact the fertility status based on the ROC findings.

scholarly journals Effect of selenium and vitamin E supplementation on reproductive performance of young boars

2005 ◽  
Vol 48 (1) ◽  
pp. 68-75 ◽  
Author(s):  
A. Kołodziej ◽  
E. Jacyno
Keyword(s):  
Vitamin E ◽  
Seminal Plasma ◽  
Reproductive Performance ◽  
Morphological Changes ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Selenium Concentration ◽  
Control Group ◽  
Young Boars ◽  
Experimental Group

Abstract. The studies were carried out on 40 young boars of the synthetic line 990. On their 70 days of age, the boars were divided into two groups; the control group received 0.2 mg Se and 30 mg vitamin E while the experimental group received 0.5 mg Se + 60 mg vitamin E per 1 kg of feed mixtures. The feeding test was carried on from 70 days until 180 days of age. During the experiment the boars were subjected to live evaluations, i.e. testes volume, libido level, semen characteristics, as well as selenium concentration and glutathione peroxidase (GSH-Px) activity in blood serum and seminal plasma. The boars of the experimental group, compared with the control, showed significantly (P ≤ 0.05) higher sperm concentration and total sperm count, significantly lower (P ≤ 0.05) percentage of spermatozoa with major or minor morphological changes, elevated (P ≤ 0.05) percentage of spermatozoa with normal acrosome, and significantly higher (P ≤ 0.01) ORT values. GSH-Px activity was higher (P ≤ 0.05) in seminal plasma of the control group boars.

scholarly journals The Role of Oxidative Stress, Selected Metals, and Parameters of the Immune System in Male Fertility

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Michał Dobrakowski ◽  
Zbigniew Kaletka ◽  
Anna Machoń-Grecka ◽  
Sławomir Kasperczyk ◽  
Stanisław Horak ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Immune System ◽  
Stress Intensity ◽  
Seminal Plasma ◽  
Antioxidant Defense ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
System Function ◽  
Immune System Function

The aim of the study was to investigate the associations between standard semen parameters and the parameters reflecting oxidative stress intensity, antioxidant defense functions, levels of selected macro and trace elements, and parameters characterizing immune system function. The study group consisted of 103 fertile males. Based on semen volume, sperm concentration, total sperm count, and percentage of motile sperm at 1 hour postcollection, the individuals were divided into two equal groups—those with excellent (EX) semen quality and those with mediocre (ME) semen quality. The remaining measured parameters characterizing motility and the percentage of normal morphology were higher in the EX group than in the ME group; however, the seminal plasma pH did not differ between the examined groups. The phosphate level was 31% lower in the EX group than in the ME group, whereas there was a tendency toward a 25% lower level of Fe in the EX group than in the ME group (p=0.064). The activities of enzymes involved in antioxidant defense, CuZn-SOD, CAT, and G6PD, were 14%, 63%, and 39%, respectively, higher in the EX group than in the ME group. However, the level of alpha-tocopherol was 32% lower in the EX group than in the ME group. The other measured parameters characterizing antioxidant defense and the parameters of oxidative stress intensity and immune system function were not significantly different. The value of seminal plasma pH is not related to the semen quality of fertile males. Higher fertility potential estimated based on standard semen parameters in fertile males is associated with lower levels of Fe and higher activities of some antioxidant enzymes.

107 Administration of antioxidants improves serum total antioxidant capacity, testosterone, and seminal quality of Magra rams

2021 ◽  
Vol 33 (2) ◽  
pp. 160
Author(s):  
A. Kumar ◽  
J. S. Mehta ◽  
A. Kumar ◽  
G. N. Purohit
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Semen Parameters ◽  
Total Antioxidant

Oxidative stress is one of the most important factors responsible for poor quality of semen, and it is defined as an imbalance between the formation of reactive oxygen species (ROS, including hydroxyl radicals, superoxide anions, and hydrogen peroxide) and antioxidant capacity of semen. Antioxidants act as a powerful defence system against ROS attack in sperm cells by decreasing oxidative stress and they improve semen quality, which could be useful in the management of male infertility. Magra is a dual-purpose (wool and meat) hardy sheep breed that originated in the eastern and southern parts of the Bikaner district of Rajasthan, India. The present study evaluated the effects of the administration of vitamin E with selenium or vitamin C on serum antioxidants, testosterone, and seminal quality of Magra rams. Twenty-one Magra rams, aged between 1.5 and 2.5 years, were randomly assigned to treatment at weekly intervals with administration of either 1mL of vitamin E (tocopherol, 50mg mL−1) and selenium (1.5mg sodium selenite; n=7; VES), 8mL of vitamin C (250mg mL−1 sodium ascorbate; n=7; VC), or 1mL of saline (n=7; control) subcutaneously for 5 consecutive weeks. After the fourth week, semen and blood were collected twice a week for 5 consecutive weeks and assayed for seminal quality parameters, serum testosterone, and serum antioxidants. Data obtained were analysed statistically using te SPSS software (version 25.0; IBM Corp.). Mean values were compared using Duncan’s multiple range test. The semen parameters sperm motility, sperm concentration, live sperm percentage, and% HOST (hypo-osmotic swelling test)-positive sperm were significantly elevated, whereas reaction time and percentage of abnormal sperm were lower in both the treated groups compared with controls (P<0.05). Seminal pH declined significantly in the VES group (P<0.05), whereas it was not different between the VC and control groups of rams (P>0.05). Glutathione peroxidase, superoxide dismutase, and total antioxidant capacity concentrations were higher in serum and seminal plasma of treated rams (P<0.05). Serum testosterone concentrations were significantly elevated in VES and VC groups (P<0.05). We concluded that the administration of vitamin E and selenium or vitamin C improves the serum testosterone production and seminal quality of rams.

Comparison of the effect of Ceratonia siliqua L. fruit oral capsule and vitamin E on semen parameters in men with idiopathic infertility: a triple-blind randomized controlled clinical trial

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Sima Sanagoo ◽  
Azizeh Farshbaf-Khalili ◽  
Parina Asgharian ◽  
Samad Hazhir ◽  
Behnaz Sadeghzadeh Oskouei
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Controlled Clinical Trial ◽  
Ceratonia Siliqua ◽  
Randomized Controlled ◽  
Infertile Men ◽  
The Mean

Abstract Objectives To compare the effect of oral Ceratonia siliqua (Carob) and vitamin E on sperm parameters in idiopathic infertile men. Methods In this triple-blind randomized controlled trial study, fifty 20–45-year-old infertile men with idiopathic abnormal sperm parameters were selected among men referring to the infertility clinic of Al Zahra-Iran Hospital, and randomly allocated into two groups. The first group received Carob capsules, 500 mg, and the second group received vitamin E capsules three times a day for 90 days. The primary outcomes of this study were the sperm parameters (count, motility, and morphology). Results After the intervention, the mean of sperm count increased from 49.08 to 60.22 million/mL in the carob vs. it increased from 47.64 to 58.88 million/mL in the vitamin E group (adjusted mean difference (aMD)=1.124 million/ml, 95% CI: −12.892 to 15.141). The mean sperm motility increased from 45.90 to 52.90% in the carob group but decreased from 55.23 to 38.10% in the vitamin E group (aMD=17.22%, 95% CI: 8.53 to 25.92). The mean sperms with normal morphology increased from 11.52 to 67.05% in the carob, and from 10.20 to 77.47% in the vitamin E group (aMD=−10.88%, 95% CI: −22.30 to 0.536). There was no serious side effect during the treatment course. Conclusions Taking 1,500 mg oral dose of Carob capsule per day for 90 days improved sperm motility but had no significant effect for improving morphology and count comparing to vitamin E capsule.

P–430 Male fertility preservation: is there a role for cancer-induced inflammation that affects semen quality in oncological patients?

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
C Omes ◽  
V Tomasoni ◽  
R Bassani ◽  
V Amico ◽  
R E Nappi
Keyword(s):  
Oxidative Stress ◽  
Fertility Preservation ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Cancer Type ◽  
Oncological Patients ◽  
Inflammatory State ◽  
Hematological Values

Abstract Study question What is the cause of semen quality impairment in oncological patients during fertility preservation programs? The cancer type and stadiation or the resulting inflammatory state? Summary answer The inflammatory state seems to be related to the decrease of sperm concentration, motility, morphology and viability due to the worsening of oxidative stress microenvironment. What is known already Fertility preservation acquired a great importance in the last decades due to increase survival of oncological patients, boost of diagnosis under 40 years and postponement of paternal age. At the time of cryopreservation, only one third of these males are normozoospermic. Tumor itself or other factors, added to psychological reasons, may be involved but there is no clear evidence. An imbalance of ROS (reactive oxygen species) in semen can compromise its quality. However, the correlation between cancer-related generalized stress state and fertility is poorly investigated. Inflammatory conditions induced by infections and pathologies, including cancer, increase ROS. Study design, size, duration Retrospective observational analysis was performed on 45 patients (29.0 ± 6.9 yrs) recruited during their fertility preservation program between 2016 and 2019 with written consent on use of their clinical data for research purpose. Patients presented several oncological diagnoses. Semen samples obtained from multiple collections (N = 58) were analyzed before applying standard freezing protocol. Data on semen parameters, inflammatory indices, hematological values and type/stage of tumors were collected. No exclusion criteria were applied. Participants/materials, setting, methods Routine semen analysis was performed according to the WHO standards. Sperm concentration and motility were evaluated on Makler Chamber, whereas eosin stain and Diff-quick slides were used for viability and morphology, respectively. Lymphoma was present in 72% of cases, leukemia in 8%, seminoma in 7% and other cancers in 13%. Correlations (Pearson/Spearman tests) among principal semen parameters and hematological values (leukocytes, erythrocytes, hemoglobin, RDW, albumin, etc.) were calculated with a P-value <0.05 considered statistically significant. Main results and the role of chance The majority of semen samples showed a severe impairment, with one or more parameters under lower reference limits (WHO): 48.3% had sperm concentration under 15 millions/ml, 43.1% had a progressive motility under 32%, 41.4% had viability under 58% and 91.4% had abnormal morphology (under 4%). The role of potential inflammatory state was analyzed by correlating semen parameters and some hematological values. No correlation was found with cancer type. Negative association resulted between progressive motility (%PR) and leukocytes (p = 0.041) or RDW% (p = 0.015), but positive one with albumin (p = 0.012). Even sperm count, total motility (%PR+NP) and morphology were significantly correlated to RDW% (p = 0.003, p = 0.032, p = 0.034, respectively). These findings suggest a possible role of inflammation and ROS related generation in semen quality impairment. Indeed, albumin exerts a protective action, but leukocytes are known to cause ROS increase. Cancer-induced oxidative stress state may alter red blood cells homeostasis and vitality and increase erythrocytes turnover resulting in high RDW values. It is likely semen is worse when blood values indicate more severe cancer-induced inflammatory condition. Limitations, reasons for caution Significant correlations with type/stage of cancer were not found due to small number of each diagnosis, in spite our study considered 3 years of patients inclusion. Moreover, we lack to analyze the same patient before the cancer onset to avoid the influence of inflammatory state generated by the tumor itself. Wider implications of the findings: Understanding the influence of cancer-induced inflammatory state on semen quality could increase the awareness that clinicians should direct patient to the fertility preservation as soon as possible, even if diagnosis is still ongoing. It should be evaluated whether offering specific treatments may reduce oxidative stress conditions. Trial registration number Not applicable

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