Detection of tumor necrosis factor (TNF-α) in cell culture medium with label free electrochemical impedance spectroscopy

2013 ◽  
Vol 181 ◽  
pp. 494-500 ◽  
Author(s):  
Tze Sian Pui ◽  
Patthara Kongsuphol ◽  
Sunil K. Arya ◽  
Tushar Bansal
Keyword(s):  
Cell Culture ◽  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Tumor Necrosis ◽  
Culture Medium ◽  
Electrochemical Impedance ◽  
Cell Culture Medium ◽  
Label Free ◽  
Tnf Α ◽  
Necrosis Factor

scholarly journals Impedimetric Detection of Femtomolar Levels of Interleukin 6, Interleukin 8, and Tumor Necrosis Factor Alpha Based on Thermally Modified Nanotubular Titanium Dioxide Arrays

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2399
Author(s):  
Katarzyna Arkusz ◽  
Ewa Paradowska
Keyword(s):  
Titanium Dioxide ◽  
Electrochemical Impedance Spectroscopy ◽  
Tumor Necrosis Factor ◽  
Impedance Spectroscopy ◽  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Electrochemical Impedance ◽  
Physical Adsorption ◽  
Interleukin 8 ◽  
Necrosis Factor

An inexpensive, easy to prepare, and label-free electrochemical impedance spectroscopy-based biosensor has been developed for the selective detection of human interleukin 6 (IL-6), interleukin 8 (CXCL8, IL-8), and tumor necrosis factor (TNFα)—potential inflammatory cancer biomarkers. We describe a, so far, newly developed and unexplored method to immobilize antibodies onto a titanium dioxide nanotube (TNT) array by physical adsorption. Immobilization of anti-IL-6, anti-IL-8, and anti-TNFα on TNT and the detection of human IL-6, IL-8, and TNFα were examined using electrochemical impedance spectroscopy (EIS). The impedimetric immunosensor demonstrates good selectivity and high sensitivity against human biomarker analytes and can detect IL-6, IL-8, and TNFα at concentrations as low as 5 pg/mL, equivalent to the standard concentration of these proteins in human blood. The calibration curves evidenced that elaborated biosensors are sensitive to three cytokines within 5 ÷ 2500 pg/mL in the 0.01 M phosphate-buffered saline solution (pH 7.4).

Label-free analysis of water-polluting parasite by electrochemical impedance spectroscopy

2010 ◽  
Vol 25 (5) ◽  
pp. 1122-1129 ◽  
Author(s):  
T. Houssin ◽  
J. Follet ◽  
A. Follet ◽  
E. Dei-Cas ◽  
V. Senez
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Label Free

DNAzyme catalytic beacons-based a label-free biosensor for copper using electrochemical impedance spectroscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (8) ◽  
pp. 6679-6685 ◽  
Author(s):  
Wenyong Hu ◽  
Xiaobo Min ◽  
Xinyu Li ◽  
Shengxiang Yang ◽  
Langbo Yi ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Gold Nanoclusters ◽  
Label Free ◽  
Selective Method

In this study, we developed a novel selective method for copper quantification based on gold nanoclusters (GNCs) and DNAzyme.

Label-Free and Ultra-Low Level Detection ofSalmonella entericaSerovar Typhimurium Using Electrochemical Impedance Spectroscopy

2009 ◽  
Vol 21 (20) ◽  
pp. 2267-2271 ◽  
Author(s):  
Jeffrey T. La Belle ◽  
Miti Shah ◽  
Justin Reed ◽  
Vivek Nandakumar ◽  
T. L. Alford ◽  
...  
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Electrochemical Impedance ◽  
Label Free ◽  
Low Level
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