Determination of Vascular Endothelial Growth Factor (VEGF) in Cell Culture Medium by Gold-Coated Magnetic Nanoparticle Based Label-Free Electrochemical Impedance Spectroscopy (EIS)

2021 ◽  
pp. 1-13
Author(s):  
Yaping Zhou ◽  
Yao Wan ◽  
Mingyu He ◽  
Ying Li ◽  
Qimei Wu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Vascular Endothelial Growth Factor ◽  
Magnetic Nanoparticle ◽  
Culture Medium ◽  
Electrochemical Impedance ◽  
Vascular Endothelial ◽  
Cell Culture Medium ◽  
Label Free ◽  
Endothelial Growth Factor

The Immobilization of VEGF-Fc for Promoting Endothelial Cells Proliferation in Porous PCL Scaffolds

2012 ◽  
Vol 554-556 ◽  
pp. 1721-1724
Author(s):  
Chuan Shun Zhu ◽  
Jian Bin Xu ◽  
Mei Hua Yu ◽  
Wei Zhao ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Vascular Endothelial Growth Factor ◽  
Culture Medium ◽  
Expression System ◽  
Glucose Consumption ◽  
Genetically Engineered ◽  
Vascular Endothelial ◽  
Cell Culture Medium ◽  
Simple Method ◽  
Endothelial Growth Factor

In this paper, a novel simple method for VEGF immobilization was developed using a new genetically engineered vascular endothelial growth factor (VEGF) fused to IgG-Fc (abbreviated as VEGF-Fc). The fusion protein of VEGF-Fc was biosynthesized by eukaryocyte expression system with the cloning plasmid encoding VEGF165 and Fc domain. Owe to the hydrophobic-bind property of the Fc domain, the VEGF-Fc is efficiently immobilized on the surface of porous polycaprolactone (PCL) scaffold in our study. The quantitative assay of VEGF-Fc immobilization by ELISA showes that the VEGF-Fc can uniformly distributed throughout the PCL scaffold and the immobilization rate is above 96% that is much higher than that of commercial VEGF. Furthermore, the proliferation of HUVECs was improved two times in the VEGF-Fc immobilized PCL scaffold comparing with that in commercial VEGF in cell culture medium, which detected by the cellular glucose consumption assays.

A novel approach for the discrimination of culture medium from Vascular Endothelial Growth Factor (VEGF) overexpressing colorectal cancer cells

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sinem Tunçer ◽  
Rafig Gurbanov
Keyword(s):  
Colorectal Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ftir Spectroscopy ◽  
Cancer Cells ◽  
Lower Amount ◽  
Vascular Endothelial ◽  
Colorectal Cancer Cells ◽  
Endothelial Growth Factor

AbstractObjectivesThe expression level of Vascular Endothelial Growth Factor (VEGF) is assumed as a prognostic marker for several tumor types, including colorectal cancer. Therefore, the determination of pre- and post-therapy levels of VEGF appears to have great value in the assessment of tumor prognosis. Enzyme-Linked Immunosorbent Assay (ELISA) is commonly used for the determination of serum or plasma VEGF levels, but the method is costly and time-consuming. In this study, we aimed to describe a rapid and cost-effective analysis method to discriminate VEGF overexpressing colorectal cancer-derived conditioned medium (CM).MethodsAttenuated Total Reflection (ATR)-Fourier Transform Infrared (FTIR) spectroscopy, combined with Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA), was used to differentiate VEGF overexpressing colorectal cancer cell line CM from CM obtained from the corresponding control cells which express and secrete relatively lower amount of VEGF.ResultsSamples belong to VEGF overexpressing colorectal cancer cells were clearly distinguished from the control group with very high PC scores as PC1 + PC2 = 96%. Besides, a 100% accurate distinction between these two groups was achieved by the LDA analysis.ConclusionsATR-FTIR spectroscopy combined with pattern recognition techniques was able to discriminate CM of VEGF overexpressing colorectal cancer cells with high efficiency and accuracy.

scholarly journals Determination of Vascular Endothelial Growth Factor (VEGF) and Tie-2 Levels in Patients with Primary Hypertension

2013 ◽  
Vol 62 (18) ◽  
pp. C93
Author(s):  
Ahmet Kemal Filiz ◽  
Hakan Güneş ◽  
Ercan Özdemir ◽  
Handan Güneş ◽  
Mehmet Birhan Yılmaz
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Primary Hypertension ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor
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