A novel rhodamine B-based lysosomal pH probe RML was developed. RML responded to acidic pH with short response time, high selectivity and high sensitivity and could detect lysosomal pH change in living cells.
A ratiometric fluorescent pH probe (DDXC) has been developed for extreme acidity, the sensing mechanism of which is based on the reversible protonation of the carbonyl oxygen followed by keto–enol tautomerization.
Traumatic brain injury (TBI) is a cause of neurodegenerative diseases accompanied by intracellular pH decrease. Herein, a FRET-based ratiometric two-photon fluorescent pH probe is designed to monitor pH change and understand TBI process.
3-[3-(4-Fluorophenyl)-1-(1-methylethyl)-1H-indol-2-yl]-(E)-2-propenal(FMIP) was used as a ratiometric fluorescent pH probe with favorable optical properties. The probe has excellent cell membrane permeability and is applied successfully to monitor pH in living cells.
We designed and synthesized a lysosome-targetable fluorescence probe, TPE-CA, which can sensitively and selectively monitor a subcellular organelle pH change.
A single design strategy for dual sensitive pH probe with a suitable range to map pH in living cells