Enhanced sensitivity of lateral flow immunoassays by using water-soluble nanofibers and silver-enhancement reactions

2018 ◽  
Vol 273 ◽  
pp. 1323-1327 ◽  
Author(s):  
Wuseok Kim ◽  
Sanghee Lee ◽  
Sangmin Jeon
Keyword(s):  
Lateral Flow ◽  
Water Soluble ◽  
Silver Enhancement ◽  
Enhanced Sensitivity

Starburst dendrimers: enhanced performance and flexibility for immunoassays

1994 ◽  
Vol 40 (9) ◽  
pp. 1845-1849 ◽  
Author(s):  
P Singh ◽  
F Moll ◽  
S H Lin ◽  
C Ferzli ◽  
K S Yu ◽  
...  
Keyword(s):  
Solid Phase ◽  
Polymeric Materials ◽  
Feasibility Studies ◽  
Solid Support ◽  
Water Soluble ◽  
Model Systems ◽  
Enhanced Sensitivity ◽  
Composition And Structure ◽  
Creatine Kinase Mb ◽  
Better Than

Abstract Starburst dendrimers are novel, water-soluble polymeric materials, with a well-defined composition and structure. In our application, we used dendrimers composed of poly(amidoamine) groups to which we coupled several specific antibodies, to investigate potential formats based on radial partition immunoassay. The coupled antibodies have retained their stability and immunological binding after coupling, both in solution and when immobilized onto a solid support. On the basis of our feasibility studies with model systems, we conclude that immunoassays can be developed with performance equivalent to or better than that in many established systems. By application of a mixture of the dendrimer-coupled antibody and the analyte of interest to the solid phase, we have investigated the performance characteristics of solution-phase immunoassays. Our experiments demonstrate enhanced sensitivity for creatine kinase MB isoenzyme (CKMB), thyrotropin, and myoglobin assays and reduced instrumental analysis time for the CKMB assay.

scholarly journals Advanced trap lateral flow immunoassay sensor for the detection of cortisol in human bodily fluids

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyun-Kyung Oh ◽  
Kihyeun Kim ◽  
Jinhee Park ◽  
Hyungjun Jang ◽  
Min-Gon Kim
Keyword(s):  
Polyvinyl Alcohol ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Ease Of Use ◽  
Water Soluble ◽  
Stress Disorders ◽  
Lateral Flow Immunoassay ◽  
Detection Range ◽  
Bodily Fluids

AbstractPaper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL−1) over a broad detection range (0.01–1000 ng∙mL−1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human real saliva samples using this sensor and a conventional ELISA (R2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.

scholarly journals Enhanced Sensitivity of Lateral Flow Tests Using a Two-Dimensional Paper Network Format

2011 ◽  
Vol 83 (20) ◽  
pp. 7941-7946 ◽  
Author(s):  
Elain Fu ◽  
Tinny Liang ◽  
Jared Houghtaling ◽  
Sujatha Ramachandran ◽  
Stephen A. Ramsey ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Two Dimensional ◽  
Enhanced Sensitivity ◽  
Lateral Flow Tests

Highly sensitive visual detection of copper (II) using water-soluble azide-functionalized gold nanoparticles and silver enhancement

2014 ◽  
Vol 59 ◽  
pp. 40-44 ◽  
Author(s):  
Zhen Zhang ◽  
Wenqing Li ◽  
Qiuling Zhao ◽  
Ming Cheng ◽  
Li Xu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Visual Detection ◽  
Water Soluble ◽  
Silver Enhancement ◽  
Functionalized Gold Nanoparticles ◽  
Highly Sensitive

scholarly journals Comparative Study of In Situ Techniques to Enlarge Gold Nanoparticles for Highly Sensitive Lateral Flow Immunoassay of SARS-CoV-2

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 229
Author(s):  
Vasily G. Panferov ◽  
Nadezhda A. Byzova ◽  
Sergey F. Biketov ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Layer ◽  
Galvanic Replacement ◽  
Silver Enhancement ◽  
Ag Nps ◽  
Au Nps ◽  
Highly Sensitive

Three techniques were compared for lowering the limit of detection (LOD) of the lateral flow immunoassay (LFIA) of the receptor-binding domain of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) based on the post-assay in situ enlargement of Au nanoparticles (Au NPs) on a test strip. Silver enhancement (growth of a silver layer over Au NPs—Au@Ag NPs) and gold enhancement (growth of a gold layer over Au NPs) techniques and the novel technique of galvanic replacement of Ag by Au in Au@Ag NPs causing the formation of Au@Ag-Au NPs were performed. All the enhancements were performed on-site after completion of the conventional LFIA and maintained equipment-free assay. The assays demonstrated lowering of LODs in the following rows: 488 pg/mL (conventional LFIA with Au NPs), 61 pg/mL (silver enhancement), 8 pg/mL (galvanic replacement), and 1 pg/mL (gold enhancement). Using gold enhancement as the optimal technique, the maximal dilution of inactivated SARS-CoV-2-containing samples increased 500 times. The developed LFIA provided highly sensitive and rapid (8 min) point-of-need testing.

Synthesis and characterisation of quantum dots coupled to mycolic acids as a water‐soluble fluorescent probe for potential lateral flow detection of antibodies and diagnosis of tuberculosis

Luminescence ◽  
2021 ◽  
Author(s):  
Kapambwe P. Kabwe ◽  
Sifiso A. Nsibande ◽  
Yolandy Lemmer ◽  
Lynne A. Pilcher ◽  
Patricia B. C. Forbes
Keyword(s):  
Quantum Dots ◽  
Fluorescent Probe ◽  
Lateral Flow ◽  
Water Soluble ◽  
Mycolic Acids ◽  
Flow Detection

scholarly journals Advanced Trap Lateral Flow Immunoassay Sensor for the Detection of Cortisol in Human Bodily Fluids

2021 ◽  
Author(s):  
Hyun-Kyung Oh ◽  
Kihyeun Kim ◽  
Hyungjun Jang ◽  
Jinhee Park ◽  
Min-Gon Kim
Keyword(s):  
Polyvinyl Alcohol ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Ease Of Use ◽  
Water Soluble ◽  
Stress Disorders ◽  
Lateral Flow Immunoassay ◽  
Detection Range ◽  
Bodily Fluids

Abstract Paper-based biosensors based on lateral flow immunoassay (LFI) are promising candidates for POC diagnosis because of their ease of use and rapid target detection. However, the low sensitivity of LFI limits its application, and signal amplification has been used in numerous studies to increase its sensitivity. We developed an advanced trap LFI (α-trapLFI), a simple-to-use sensor, with an additional step for signal amplification. Here, signal amplification is automatically implemented following delayed release of enhancement solution induced by water-soluble polyvinyl alcohol tape. As the polyvinyl alcohol tape is exposed to water, its polymer structure is perturbed (within 5 min), allowing ions to pass through. This new sensor was designed to have a short time delay between the flow of solutions used for the immunoassay and signal amplification. The α-trapLFI was subsequently used to detect cortisol with high sensitivity (9.1 pg∙mL-1) over a broad detection range (0.01–1000 ng∙mL-1) in bodily fluids. Furthermore, an excellent correlation was obtained by analyzing 20 human saliva samples using this sensor and a conventional ELISA (R2 = 0.90). The new sensor will be helpful in detecting various small molecules for simple, rapid, and portable POC diagnosis of stress disorders.

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