A label-free impedimetric immunosensor for direct determination of the textile dye Disperse Orange 1

Talanta ◽  
2015 ◽  
Vol 142 ◽  
pp. 183-189 ◽  
Author(s):  
Jing Yang ◽  
Carolina Gomes da Rocha ◽  
Shengfu Wang ◽  
Antonio Aparecido Pupim Ferreira ◽  
Hideko Yamanaka
Keyword(s):  
Direct Determination ◽  
Textile Dye ◽  
Label Free ◽  
Impedimetric Immunosensor

Encapsulation of hemin in Fe-based metal-organic frameworks and its application for the direct determination of aflatoxin M1

2021 ◽  
pp. 1-10
Author(s):  
F. Jahangiri-Dehaghani ◽  
H.R. Zare ◽  
Z. Shekari
Keyword(s):  
Electrochemical Impedance ◽  
Metal Organic Frameworks ◽  
Direct Determination ◽  
Differential Pulse ◽  
Aflatoxin M1 ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Selective Determination ◽  
Metal Organic

A label-free electrochemical aptasensor was constructed for the sensitive and selective determination of AFM1. For preparation of the aptasensor, the AFM1 aptamer was immobilised on the surface of a glassy carbon electrode modified with hemin encapsulated in Fe-based metal-organic frameworks (hemin@Fe-MIL-101). The morphology and the structure of Fe-MIL-101 and hemin@Fe-MIL-101 were evaluated by scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction and Brunauer-Emmett-Teller-N2 sorption methods. Electrochemical impedance spectroscopy and cyclic voltammetry were performed to monitor the fabrication process of the electrochemical aptasensor. The electrochemical reduction current of hemin encapsulated in Fe-MIL-101 serves as a signal for the quantitative determination of AFM1. Differential pulse voltammetry was done to determine the AFM1 concentration in the linear range of 1.0×10-1-100.0 ng/ml. The detection limit of AFM1 was estimated to be 4.6×10-2 ng/ml. Finally, the fabricated aptasensor was applied to determine AFM1 in raw and boiled milk samples.

Label-Free Amperometric Magnetoimmunosensors for Direct Determination of Lactoperoxidase in Milk

2013 ◽  
Vol 25 (4) ◽  
pp. 967-974 ◽  
Author(s):  
Rebeca M. Torrente-Rodríguez ◽  
Susana Campuzano ◽  
María Gamella ◽  
Felipe Conzuelo ◽  
A. Julio Reviejo ◽  
...  
Keyword(s):  
Direct Determination ◽  
Label Free

Study on the Novel Label Free Impedimetric Immunosensor for Aflatoxin B1 Based on Poly(o-phenylenediamine) Film Modified Gold Electrode

2011 ◽  
Vol 322 ◽  
pp. 385-389 ◽  
Author(s):  
Ning Ning Wu ◽  
Li Xin Cao ◽  
Pei Sheng Yan ◽  
Ming Hao Wang
Keyword(s):  
Aflatoxin B1 ◽  
Gold Electrode ◽  
Biomolecular Recognition ◽  
The Novel ◽  
Label Free ◽  
Buffer Solution ◽  
Solution Ph ◽  
Linear Detection ◽  
Impedimetric Immunosensor

A label free impedimetric immunosensor for the determination of aflatoxin B1 (AFB1) was fabricated by immobilizing anti-AFB1 onto Poly o-phenylenediamine (PoPD) electropolymerized film modified gold electrode by glutaraldehyde (GA) cross-linking. An electrochemical interfacial modeling of biomolecular recognition was recommended and reasonably interpreted. EIS technology was employed for the quantitative determination. The linear detection concentration ranges of AFB1 were 0.03~0.1 μg/mL and 0.1~0.7 μg/mL. The detection limit was 2.8×10-8μg/mL. The immunosensor could be reused more than 10 times when renewed by HCl-Glycine buffer solution (pH 2.6).

scholarly journals Electrochemical-Based Serological Detection of Bovine Immunoglobulin G in Calves

2020 ◽  
Author(s):  
Caoimhe Robinson ◽  
Niamh Creedon ◽  
Riona sayers ◽  
emer kennedy ◽  
Alan O'Riordan
Keyword(s):  
Immunoglobulin G ◽  
Rapid Determination ◽  
Passive Transfer ◽  
Label Free ◽  
Serological Detection ◽  
New Born ◽  
Risk Of Mortality ◽  
Bovine Immunoglobulin ◽  
Impedimetric Immunosensor

Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus,<i> in utero</i>. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 24 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficent detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves’ vitality and survival rate

scholarly journals Electrochemical-Based Serological Detection of Bovine Immunoglobulin G in Calves

2020 ◽  
Author(s):  
Caoimhe Robinson ◽  
Niamh Creedon ◽  
Riona sayers ◽  
emer kennedy ◽  
Alan O'Riordan
Keyword(s):  
Immunoglobulin G ◽  
Rapid Determination ◽  
Passive Transfer ◽  
Label Free ◽  
Serological Detection ◽  
New Born ◽  
Risk Of Mortality ◽  
Bovine Immunoglobulin ◽  
Impedimetric Immunosensor

Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus,<i> in utero</i>. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 24 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficent detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves’ vitality and survival rate

The Direct Determination of I131 in Heterogeneous Fecal Specimens

1961 ◽  
Vol 41 (4) ◽  
pp. 380-384 ◽  
Author(s):  
Arthur F. Dratz ◽  
James C. Coberly
Keyword(s):  
Direct Determination
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