scholarly journals Electrochemical-Based Serological Detection of Bovine Immunoglobulin G in Calves

2020 ◽  
Author(s):  
Caoimhe Robinson ◽  
Niamh Creedon ◽  
Riona sayers ◽  
emer kennedy ◽  
Alan O'Riordan
Keyword(s):  
Immunoglobulin G ◽  
Rapid Determination ◽  
Passive Transfer ◽  
Label Free ◽  
Serological Detection ◽  
New Born ◽  
Risk Of Mortality ◽  
Bovine Immunoglobulin ◽  
Impedimetric Immunosensor

Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus,<i> in utero</i>. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 24 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficent detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves’ vitality and survival rate

scholarly journals Electrochemical-Based Serological Detection of Bovine Immunoglobulin G in Calves

2020 ◽  
Author(s):  
Caoimhe Robinson ◽  
Niamh Creedon ◽  
Riona sayers ◽  
emer kennedy ◽  
Alan O'Riordan
Keyword(s):  
Immunoglobulin G ◽  
Rapid Determination ◽  
Passive Transfer ◽  
Label Free ◽  
Serological Detection ◽  
New Born ◽  
Risk Of Mortality ◽  
Bovine Immunoglobulin ◽  
Impedimetric Immunosensor

Bovine antibodies, such as immunoglobulin G (IgG), cannot pass the placental barrier and as such are not transferred from the mother to the foetus,<i> in utero</i>. Instead a calf must absorb antibodies following ingestion of colostrum postpartum. Failure of Passive Transfer (FPT) is a condition that predisposes calves to development of disease and increases the risk of mortality. Thus, continuous early monitoring of IgG absorption in a calf, within the first 24 hours of life, is imperative to allow faster treatment and prevent FPT. In this paper, we present the development of a label-free impedimetric immunosensor device for bovine IgG in serum and demonstrate its suitability to determine early FPT in new-born calves. The developed sensors were challenged to discriminate between new born calf sera, both pre- and post-colostrum feeding, and demonstrated efficent detection of IgG in under 15 minutes. Such a device could enable rapid determination of FPT, thereby improving calves’ vitality and survival rate

Incidence of Colostrum in Raw Milk

1993 ◽  
Vol 56 (7) ◽  
pp. 625-626 ◽  
Author(s):  
JERZY ZAWISTOWSKI ◽  
RUFINA MACKINNON
Keyword(s):  
Immunoglobulin G ◽  
Raw Milk ◽  
Radial Immunodiffusion ◽  
Milk Samples ◽  
Bovine Immunoglobulin

A survey was conducted to determine the colostrum content in raw milk from dairies in Manitoba, Canada. Colostrum was indirectly measured by the determination of bovine immunoglobulin G (IgG) using a radial immunodiffusion assay. The results showed that 360 milk samples, which accounted for 89% of the total tested samples, were contaminated with colostrum. Of these, 320 samples had IgG levels in the range of 1.0 to 1.5 mg/ml, while 38 samples had an IgG content in the range of 1.5 to 2.0 mg/ml. Two milk samples contained IgG in excess of 2 mg/ml.

scholarly journals Determination of the Presence of Bovine Immunoglobulin G in Liquid or Spray-Dried Porcine Plasma and Whole Blood by Agar Gel Immunodiffusion

2004 ◽  
Vol 87 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Javier Polo ◽  
Neus Saborido ◽  
Jesús Ródenas ◽  
Carmen Rodríguez
Keyword(s):  
Immunoglobulin G ◽  
Whole Blood ◽  
Animal Feed ◽  
Cross Contamination ◽  
Agar Gel ◽  
Laboratory Equipment ◽  
Before And After ◽  
Bovine Immunoglobulin ◽  
Blood Centrifugation

Abstract There is currently urgent interest in identifying the species of origin of the components of different animal by-products. In Europe, this interest is expected to increase with authorization of the re-introduction of these proteins into animal feed formulations. The number of validated methods to differentiate the species of origin for most of these products is limited. An easy, inexpensive, and accurate test was developed to determine the cross-contamination of bovine blood or plasma in porcine whole blood and plasma, both before and after spray drying. Agar gel immunodiffusion (AGID), the studied technique, detected the presence of bovine immunoglobulin G (IgG) in porcine plasma and in whole blood at inclusion levels &gt;0.5% (v/v) in all cases. However, detectability was lower in liquid plasma (0.3%, v/v) and in liquid whole blood (0.5%, v/v). No differences were found when cross-contamination was simulated before or after whole blood centrifugation. The method described is reliable and inexpensive, and the samples are easy to prepare. Both minimal laboratory equipment and expertise are required for detection of bovine IgG in porcine blood products at inclusion levels of &gt;0.5% (v/v).

scholarly journals Rapid Determination of Ochratoxin A in Grape and Its Commodities Based on a Label-Free Impedimetric Aptasensor Constructed by Layer-by-Layer Self-Assembly

Toxins ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 71 ◽  
Author(s):  
Mina Nan ◽  
Yang Bi ◽  
Huali Xue ◽  
Sulin Xue ◽  
Haitao Long ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Self Assembly ◽  
Electrochemical Impedance ◽  
Rapid Determination ◽  
Grape Juice ◽  
High Sensitivity ◽  
Layer By Layer ◽  
Label Free ◽  
Transfer Resistance

A simple and sensitive label-free impedimetric aptasensor for rapid determination of ochratoxin A (OTA) has been developed, which was based on the combination between thiolated aptamer and gold nanoparticles by layer-by-layer self-assembly. Because of the interaction between aptamer and OTA, the relative normalized electron-transfer resistance (ΔRct) values obtained by electrochemical impedance spectroscopy (EIS) was proportional to the concentration of OTA and showed a good linear relationship from 0.1 to 10.0 ng/mL, with a lower detection limit (0.030 ng/mL) than one-step thiolated DNA aptasensor. The established method was successfully applied to detect and analyze OTA in table wine and grape juice, and the recovery was 90.56%–104.21% when PVP effective removed of phenolic substances. The label-free impedimetric aptasensor was used for rapid detection and quantitation of OTA in the inoculated grapes with the Aspergillus Nigri (H1), and the production of OTA (62.4 μg/kg, 20 μg/kg) far exceeded the maximum levels of 2 μg/kg after inoculation for three days. The developed method exhibited a good specificity, high sensitivity, time-efficient, and it could be applied to detect the OTA concentration in grape and its commodities.

scholarly journals Using N-doped Carbon Dots Prepared Rapidly by Microwave Digestion as Nanoprobes and Nanocatalysts for Fluorescence Determination of Ultratrace Isocarbophos with Label-Free Aptamers

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 223 ◽  
Author(s):  
Xin Li ◽  
Xin Jiang ◽  
Qingye Liu ◽  
Aihui Liang ◽  
Zhiliang Jiang
Keyword(s):  
Catalytic Activity ◽  
Detection Limit ◽  
Fluorescent Probe ◽  
Carbon Dots ◽  
Rapid Determination ◽  
Microwave Digestion ◽  
Label Free ◽  
Laser Scattering ◽  
Doped Carbon Dots

The strongly fluorescent and highly catalytic N-doped carbon dots (CDN) were rapidly prepared by a microwave irradiation procedure and were characterized by electron microscopy (EM), laser scattering, infrared spectroscopy (IR), and by their fluorescence spectrum. It was found that the CDN had a strong catalytic effect on the fluorescence reaction of 3,3′,5,5′-tetramethylbenzidine hydroxide ((TMB)–H2O2) which produced the oxidation product of TMB (TMBOX) with strong fluorescence at 406 nm. The aptamer (Apt) was adsorbed on the CDN surfaces which weakened the fluorescence intensity due to the inhibition of catalytic activity. When the target molecule isocarbophos (IPS) was added, it reacted with the Apt to form a stable conjugate and free CDN which restored the catalytic activity to enhance the fluorescence. Using TMBOX as a fluorescent probe, a highly sensitive nanocatalytic method for determination of 0.025–1.5 μg/L IPS was established with a detection limit of 0.015 μg/L. Coupling the CDN fluorescent probe with the Apt–IPS reaction, a new CD fluorescence method was established for the simple and rapid determination of 0.25–1.5 μg/L IPS with a detection limit of 0.11 μg/L.

A label-free impedimetric immunosensor for direct determination of the textile dye Disperse Orange 1

Talanta ◽  
2015 ◽  
Vol 142 ◽  
pp. 183-189 ◽  
Author(s):  
Jing Yang ◽  
Carolina Gomes da Rocha ◽  
Shengfu Wang ◽  
Antonio Aparecido Pupim Ferreira ◽  
Hideko Yamanaka
Keyword(s):  
Direct Determination ◽  
Textile Dye ◽  
Label Free ◽  
Impedimetric Immunosensor

scholarly journals Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum

2017 ◽  
Vol 410 (3) ◽  
pp. 981-988 ◽  
Author(s):  
Inês I. Ramos ◽  
Luís M. Magalhães ◽  
Luisa Barreiros ◽  
Salette Reis ◽  
José L. F. C. Lima ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunoglobulin G ◽  
Label Free ◽  
Bead Injection ◽  
Automated Determination

scholarly journals Affinity Liquid Chromatography Method for the Quantification of Immunoglobulin G in Bovine Colostrum Powders

2006 ◽  
Vol 89 (5) ◽  
pp. 1249-1256 ◽  
Author(s):  
David E J Copestake ◽  
Harvey E Indyk ◽  
Don E Otter
Keyword(s):  
Liquid Chromatography ◽  
Immunoglobulin G ◽  
Reversed Phase ◽  
Chromatography Method ◽  
Relative Standard ◽  
Liquid Chromatography Method ◽  
And Performance ◽  
Bovine Immunoglobulin ◽  
Reversed Phase Lc

Abstract An affinity liquid chromatography (LC) method for the determination of bovine immunoglobulin G (IgG), using protein G coupled to an agarose support, was modified to permit the quantification of IgG in colostrum-based powders. Sample preparation included pH adjustment to 4.6 to precipitate casein and denatured whey protein. The method was applied to a range of colostrum powders and was compared with the alternative independent methods of surface plasmon resonance immunoassay, radial immunodiffusion, and reversed-phase LC. The method was rapid, and performance parameters included a working range of 10-150 μg IgG and precision relative standard deviation values of &lt;10%.

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