Development of the sensitive lateral flow immunoassay with silver enhancement for the detection of Ralstonia solanacearum in potato tubers

Talanta ◽  
2016 ◽  
Vol 152 ◽  
pp. 521-530 ◽  
Author(s):  
Vasily G. Panferov ◽  
Irina V. Safenkova ◽  
Yury A. Varitsev ◽  
Natalia V. Drenova ◽  
Konstantin P. Kornev ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Potato Tubers ◽  
Silver Enhancement

scholarly journals Comparative Study of In Situ Techniques to Enlarge Gold Nanoparticles for Highly Sensitive Lateral Flow Immunoassay of SARS-CoV-2

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 229
Author(s):  
Vasily G. Panferov ◽  
Nadezhda A. Byzova ◽  
Sergey F. Biketov ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Layer ◽  
Galvanic Replacement ◽  
Silver Enhancement ◽  
Ag Nps ◽  
Au Nps ◽  
Highly Sensitive

Three techniques were compared for lowering the limit of detection (LOD) of the lateral flow immunoassay (LFIA) of the receptor-binding domain of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) based on the post-assay in situ enlargement of Au nanoparticles (Au NPs) on a test strip. Silver enhancement (growth of a silver layer over Au NPs—Au@Ag NPs) and gold enhancement (growth of a gold layer over Au NPs) techniques and the novel technique of galvanic replacement of Ag by Au in Au@Ag NPs causing the formation of Au@Ag-Au NPs were performed. All the enhancements were performed on-site after completion of the conventional LFIA and maintained equipment-free assay. The assays demonstrated lowering of LODs in the following rows: 488 pg/mL (conventional LFIA with Au NPs), 61 pg/mL (silver enhancement), 8 pg/mL (galvanic replacement), and 1 pg/mL (gold enhancement). Using gold enhancement as the optimal technique, the maximal dilution of inactivated SARS-CoV-2-containing samples increased 500 times. The developed LFIA provided highly sensitive and rapid (8 min) point-of-need testing.

scholarly journals Increased sensitivity of lateral flow immunoassay for ochratoxin A through silver enhancement

2013 ◽  
Vol 405 (30) ◽  
pp. 9859-9867 ◽  
Author(s):  
L. Anfossi ◽  
F. Di Nardo ◽  
C. Giovannoli ◽  
C. Passini ◽  
C. Baggiani
Keyword(s):  
Ochratoxin A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Enhancement ◽  
Increased Sensitivity

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Upconversion nanoparticles-based lateral flow immunoassay for point-of-care diagnosis of periodontitis

2021 ◽  
Vol 334 ◽  
pp. 129673
Author(s):  
Wanghong He ◽  
Minli You ◽  
Zedong Li ◽  
Lei Cao ◽  
Feng Xu ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Upconversion Nanoparticles

scholarly journals Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Sensors ◽  
2021 ◽  
Vol 21 (15) ◽  
pp. 5185
Author(s):  
Fabio Di Nardo ◽  
Matteo Chiarello ◽  
Simone Cavalera ◽  
Claudio Baggiani ◽  
Laura Anfossi
Keyword(s):  
Environmental Control ◽  
Improve Patient Care ◽  
Turnaround Time ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Future Perspectives ◽  
Immunoassay Technique ◽  
Food And Feed ◽  
Application Fields ◽  
Feed Safety

The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform the on-site detection of target substances. LFIA can be considered as a sort of lab-in-a-hand and, together with other point-of-need tests, has represented a paradigm shift from sample-to-lab to lab-to-sample aiming to improve decision making and turnaround time. The features of LFIAs made them a very attractive tool in clinical diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. As a consequence, from the detection of molecules, organisms, and (bio)markers for clinical purposes, the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. This review aims to provide readers with a 10-years overview of applications, outlining the trends for the main application fields and the relative compounded annual growth rates. Moreover, future perspectives and challenges are discussed.

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