scholarly journals Increased sensitivity of lateral flow immunoassay for ochratoxin A through silver enhancement

2013 ◽  
Vol 405 (30) ◽  
pp. 9859-9867 ◽  
Author(s):  
L. Anfossi ◽  
F. Di Nardo ◽  
C. Giovannoli ◽  
C. Passini ◽  
C. Baggiani
Keyword(s):  
Ochratoxin A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Enhancement ◽  
Increased Sensitivity

scholarly journals Rapid and Simple Detection of Ochratoxin A using Fluorescence Resonance Energy Transfer on Lateral Flow Immunoassay (FRET-LFI)

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 292
Author(s):  
Hyun-Kyung Oh ◽  
Hyou-Arm Joung ◽  
Minhyuk Jung ◽  
Hohjai Lee ◽  
Min-Gon Kim
Keyword(s):  
Energy Transfer ◽  
Ochratoxin A ◽  
Fluorescence Resonance Energy Transfer ◽  
Matrix Effect ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
The Matrix ◽  
Fluorescence Resonance

The detection of mycotoxins is crucial because of their toxicity in plants, animals, and humans. It is very important to determine whether food products are contaminated with mycotoxins such as ochratoxin A (OTA), as mycotoxins can survive heat treatments and hydrolysis. In this study, we designed a fluorescence resonance energy transfer (FRET)-based system that exploits antibody-antigen binding to detect mycotoxins more rapidly and easily than other currently available methods. In addition, we were able to effectively counteract the matrix effect in the sample by using a nitrocellulose membrane that enabled fluorescence measurement in coffee samples. The developed FRET on lateral flow immunoassay (FRET-LFI) system was used to detect OTA at a limit of detection (LOD) of 0.64 ng∙mL−1, and the test can be completed in only 30 min. Moreover, OTA in coffee samples was successfully detected at a LOD of 0.88 ng∙mL−1, overcoming the matrix effect, owing to the chromatographic properties of the capillary force of the membrane. We believe that the developed system can be used as a powerful tool for the sensitive diagnosis of harmful substances such as mycotoxins and pesticides for environmental and food quality control monitoring.

A Lateral Flow Immunoassay for the Rapid Detection of Ochratoxin A in Wine and Grape Must

2012 ◽  
Vol 60 (46) ◽  
pp. 11491-11497 ◽  
Author(s):  
Laura Anfossi ◽  
Cristina Giovannoli ◽  
Gianfranco Giraudi ◽  
Flavia Biagioli ◽  
Cinzia Passini ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Grape Must

A lateral flow immunoassay for measuring ochratoxin A: Development of a single system for maize, wheat and durum wheat

Food Control ◽  
2011 ◽  
Vol 22 (12) ◽  
pp. 1965-1970 ◽  
Author(s):  
Anfossi Laura ◽  
D’Arco Gilda ◽  
Baggiani Claudio ◽  
Giovannoli Cristina ◽  
Giraudi Gianfranco
Keyword(s):  
Durum Wheat ◽  
Ochratoxin A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Single System

scholarly journals Comparative Study of In Situ Techniques to Enlarge Gold Nanoparticles for Highly Sensitive Lateral Flow Immunoassay of SARS-CoV-2

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 229
Author(s):  
Vasily G. Panferov ◽  
Nadezhda A. Byzova ◽  
Sergey F. Biketov ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Limit Of Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Silver Layer ◽  
Galvanic Replacement ◽  
Silver Enhancement ◽  
Ag Nps ◽  
Au Nps ◽  
Highly Sensitive

Three techniques were compared for lowering the limit of detection (LOD) of the lateral flow immunoassay (LFIA) of the receptor-binding domain of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) based on the post-assay in situ enlargement of Au nanoparticles (Au NPs) on a test strip. Silver enhancement (growth of a silver layer over Au NPs—Au@Ag NPs) and gold enhancement (growth of a gold layer over Au NPs) techniques and the novel technique of galvanic replacement of Ag by Au in Au@Ag NPs causing the formation of Au@Ag-Au NPs were performed. All the enhancements were performed on-site after completion of the conventional LFIA and maintained equipment-free assay. The assays demonstrated lowering of LODs in the following rows: 488 pg/mL (conventional LFIA with Au NPs), 61 pg/mL (silver enhancement), 8 pg/mL (galvanic replacement), and 1 pg/mL (gold enhancement). Using gold enhancement as the optimal technique, the maximal dilution of inactivated SARS-CoV-2-containing samples increased 500 times. The developed LFIA provided highly sensitive and rapid (8 min) point-of-need testing.

A novel magneto-gold nanohybrid-enhanced lateral flow immunoassay for ultrasensitive and rapid detection of ochratoxin A in grape juice

2021 ◽  
Vol 336 ◽  
pp. 127710
Author(s):  
Liangwen Hao ◽  
Jing Chen ◽  
Xirui Chen ◽  
Tongtong Ma ◽  
Xiaoxia Cai ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Rapid Detection ◽  
Grape Juice ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

Development of the sensitive lateral flow immunoassay with silver enhancement for the detection of Ralstonia solanacearum in potato tubers

Talanta ◽  
2016 ◽  
Vol 152 ◽  
pp. 521-530 ◽  
Author(s):  
Vasily G. Panferov ◽  
Irina V. Safenkova ◽  
Yury A. Varitsev ◽  
Natalia V. Drenova ◽  
Konstantin P. Kornev ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Potato Tubers ◽  
Silver Enhancement

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

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