Establishment of a specific radioimmunoassay for bovine interferon τ

Specific Activities

ABSTRACT The preparation of radioiodinated human LH and human FSH with specific activities of 50—150 μc/μg for use in specific radioimmunoassay systems is described. Methods for minimising iodination damage, for removing severely damaged fractions after iodination and for assessing the immunological reactivity of the final products are detailed. The obligation upon immunoassayists to demonstrate that their labelled preparations do indeed represent the hormones under consideration is discussed and criteria which may contribute evidence on this question are put forward.

Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20020010 ◽

2002 ◽

Vol 67 (1) ◽

pp. 10-18 ◽

Cited By ~ 1

Author(s):

Richard Hampl ◽

Martin Hill ◽

Luboslav Stárka

Keyword(s):

Healthy Subjects ◽

Serum Levels ◽

Body Fluids ◽

Gas Chromatography Mass Spectrometry ◽

Order Of Magnitude ◽

Three Body ◽

First Time ◽

Nanomolar Range ◽

Detection And Quantification

3β,7α-Dihydroxyandrost-5-en-17-one (1) (7α-OH-DHEA) and its 7β-hydroxy epimer 2 (7β-OH-DHEA) - 7α- and 7β-hydroxydehydroepiandrosterone - were detected and quantified in three human body fluids: in blood serum, saliva and ejaculate. Specific radioimmunoassay and gas chromatography-mass spectrometry have been used. For the first time the data on changes of these dehydroepiandrosterone metabolites are reported for a representative group of healthy subjects of both sexes (172 females and 217 males) during the life span. The serum levels of both 7-hydroxydehydroepiandrosterone epimers in serum and also in semen were in the low nanomolar range, while concentrations by one order of magnitude lower were found in saliva, but still within the detection limit. The results will serve as a basis for comparative studies of 7-hydroxydehydroepiandrosterone levels under various pathophysiological conditions, with a particular respect to autoimmune disorders.

Measurement of Leukotriene Release from Rat Peritoneal Cells with a Specific Radioimmunoassay

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1984.tb13744.x ◽

1984 ◽

Vol 435 (1 First Colloqu) ◽

pp. 113-115

Author(s):

DAVID AHARONY ◽

PAUL DOBSON ◽

PETER BERNSTEIN ◽

ROBERT D. KRELL ◽

J. BRYAN SMITH

Keyword(s):

Peritoneal Cells ◽

Immunochemical analysis of cartilage proteoglycans. Radioimmunoassay of the molecules and the substructures

Biochemical Journal ◽

10.1042/bj1870687 ◽

1980 ◽

Vol 187 (3) ◽

pp. 687-694 ◽

Cited By ~ 19

Author(s):

J Wieslander ◽

D Heinegárd

Keyword(s):

Hyaluronic Acid ◽

Uronic Acid ◽

Chondroitin Sulphate ◽

Relative Content ◽

Binding Region ◽

Rich Region ◽

Link Protein ◽

Cartilage Proteoglycans ◽

Hyaluronic Acid Binding

Antibodies specifically reacting with the link proteins, the hyaluronic acid-binding region and chondroitin sulphate-peptides were used to design specific radioimmunoassay procedures. The sensitivity of the method used for the link protein was about 20 ng/ml, and the other two components could be determined at concentrations of about 2 ng/ml. The radioimmunoassay procedures were tested by using proteoglycan subfractions or fragments thereof. The procedures used to quantify link protein and hyaluronic acid-binding region showed no cross-interference. Fragments of trypsin-digested proteoglycan monomers still reacted in the radioimmunoassay for hyaluronic acid-binding region. Subfractions of proteoglycan monomers separated according to size had a gradually higher relative content of the hyaluronic acid-binding region compared with both chondroitin sulphate-peptides and uronic acid, when the molecules were smaller. The proteoglycans therefore may contain a variably large chondroitin sulphate-rich region, which has a constant substitution with polysaccharide side chains.

Validation of a specific radioimmunoassay to measure plasma cortisol in the fetal sheep

Steroids ◽

10.1016/s0039-128x(79)80005-7 ◽

1979 ◽

Vol 33 (1) ◽

pp. 45-54 ◽

Cited By ~ 15

Author(s):

David M. Magyar ◽

John E. Buster ◽

Carlene W. Eisner ◽

Peter W. Nathanielsz ◽

Rene Oliveros ◽

...

Keyword(s):

Plasma Cortisol ◽

Fetal Sheep ◽

Development of a specific radioimmunoassay for cortisol 17-butyrate

Steroids ◽

10.1016/s0039-128x(83)90060-0 ◽

1983 ◽

Vol 42 (1) ◽

pp. 23-36

Author(s):

G.N. Smith ◽

Y. Foon Lee ◽

D.E. Bu'Lock ◽

P. August ◽

D.C. Anderson

Keyword(s):

Determination of genetic engineered insulin precursors from Escherichia coli by means of a carboxy terminus-specific radioimmunoassay

Analytica Chimica Acta ◽

10.1016/0003-2670(91)87033-4 ◽

1991 ◽

Vol 249 (1) ◽

pp. 271-278 ◽

Cited By ~ 1

Author(s):

S Müllner ◽

W König ◽

H.-P Neubauer ◽

M Schmalz ◽

D Tripier

Keyword(s):

Escherichia Coli ◽

Carboxy Terminus ◽

COMPARATIVE IMMUNOCHEMICAL CHARACTERIZATION OF PRORENIN/RENIN IN HUMAN EXTRARENAL TISSUES AND PLASMA WITH USE OF TWO SPECIFIC RADIOIMMUNOASSAY SYSTEMS

Biomedical Research ◽

10.2220/biomedres.16.371 ◽

1995 ◽

Vol 16 (6) ◽

pp. 371-380

Author(s):

KAZUSABURO KATAOKA ◽

NOBUO KUROKAWA ◽

MASAYOSHI SAWADA ◽

KAZUAKI IGUCHI ◽

NOBORU YANAIHARA ◽

...

Keyword(s):

Immunochemical Characterization ◽

Formation and Distribution of Sennosides in Cassia angustifolia, as Determined by a Sensitive and Specific Radioimmunoassay*

Planta Medica ◽

10.1055/s-2007-971666 ◽

1981 ◽

Vol 41 (01) ◽

pp. 1-14 ◽

Cited By ~ 19

Author(s):

R. Atzorn ◽

E. Weiler ◽

M. Zenk

Keyword(s):

Cassia Angustifolia ◽

The development of the hypothalamo–pituitary axis in the neonatal rat: sexual maturation in male and female rats as assessed by hypothalamic LHRH and pituitary and serum LH and FSH concentrations

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y77-149 ◽

1977 ◽

Vol 55 (5) ◽

pp. 1091-1097 ◽

Cited By ~ 20

Author(s):

J. H. Dussault ◽

P. Walker ◽

J. D. Dubois ◽

F. Labrie

Keyword(s):

Developmental Process ◽

Neonatal Period ◽

Neonatal Rat ◽

Female Rats ◽

Human Foetus ◽

Marked Rise ◽

Male And Female Rats ◽

Serum Lh ◽

And Control

Using specific radioimmunoassay techniques, we have measured hypothalamic LHRH concentration and pituitary and serum LH and FSH concentrations in neonatal rats from 0 to 60 days after birth. There were no sex differences demonstrable for hypothalamic LHRH concentration, which rose from minimal values at 2 days to peak concentrations at 22–28 days and declined significantly with the approach of puberty. Pituitary LH concentration in the two sexes rose to peak values at 19–25 days, with significantly higher values observed in females (p < 0.01). Serum LH concentration was high in both sexes at birth, the values in females being significantly higher than those observed in males (p < 0.01). While the serum LH concentration remained relatively stable throughout the study period in males, it declined rapidly to a nadir at 28 days in females. Pituitary FSH concentration was low at birth in both sexes. Females demonstrated a marked rise to peak concentrations at 16 days while males achieved significantly lower peak prepuberal values (p < 0.01) at 28 days. Serum FSH concentration was significantly higher in females (p < 0.01) at birth and rose further to peak values at 16 days. Males attained peak serum FSH concentrations at 35 days. These data demonstrate that the maturation of the hypothalamo–pituitary–gonadal axis develops in the neonatal period in the rat and confirm the presence of significant differences in hypothalamic sexual differentiation and control. The possibility of this developmental process in the neonatal rat as a model for the study of the midgestational hypothalamic maturation of the human foetus is discussed.