Expression of cyclooxygenase-2 in the canine lower urinary tract with regard to the effects of gonadal status and gender

2009 ◽  
Vol 71 (8) ◽  
pp. 1276-1288 ◽  
Author(s):  
S. Ponglowhapan ◽  
D.B. Church ◽  
M. Khalid
2001 ◽  
Vol 56 (5-6) ◽  
pp. 455-463 ◽  
Author(s):  
Cheng Luo ◽  
Leena Strauss ◽  
Ari Ristimäki ◽  
Tomi Streng ◽  
Risto Santti

Expression of cyclooxygenase-2 (E. C. 1.14.99.1) in prostate and the lower urinary tract (LUT) of the neonatally estrogenized male rat has been studied by using a COX-2 ’s PCR fragment of 724 nt spanning 3 introns and a 478nt internal standard for quantitative RTPCR. The same fragment of 724 nt was used for RNA probe in Northern hybridization. Neonatal estrogenization (10 μg/day of diethylstilbestrol on days 1-5) had no effect on COX -2 expression in prostatic urethra, prostatic lobes, or bladder. Acute estrogen treatment of castrated animals did not induce COX-2 expression, either. In addition the differential expression of basal level of COX-2 in the different lobes of prostate in normal rat was demonstrated. Our results suggest a constant expression of COX-2 gene in prostate and the lower urinary tract of the neonatally estrogenized (neoDES) rats. The present study indicates that the increased expression of COX -2 is probably not essential for the estrogen-driven development of stromal inflammation or hyperplastic and dysplastic alterations in the prostate of neoDES rats.


Author(s):  
Richard J Bryant ◽  
James W Catto

Haematuria is the presence of blood within the urine and is best defined as visible or non-visible, and symptomatic or non-symptomatic. Non-visible haematuria may be further classified as microscopic or dipstick, depending on the investigation used to detect it. Around 40% of patients presenting with visible haematuria will be found to have a significant underlying cause, and around 20% of patients will be found to have an underlying urological malignancy. Many younger patients will be found to have an underlying nephrological disease. The risk of significant underlying malignancy increases with the extent of haematuria (visible versus non-visible), the absence of symptoms and various patient factors (such as rising age, smoking status, and gender). As such, haematuria is an important urological symptom that requires investigation. These investigations should include clinical assessment, urinalysis, lower urinary tract endoscopy, and upper urinary tract radiological evaluation.


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