Maturation of spermatozoa from rainbow trout (Oncorhynchus mykiss) sex-reversed females using artificial seminal plasma or glucose–methanol extender

2015 ◽  
Vol 83 (7) ◽  
pp. 1213-1218 ◽  
Author(s):  
Andrzej Ciereszko ◽  
Grzegorz J. Dietrich ◽  
Joanna Nynca ◽  
Stefan Dobosz ◽  
Janusz Krom
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Seminal Plasma ◽  
Rainbow Trout Oncorhynchus Mykiss

Androgenetic diploids of rainbow trout (Oncorhynchus mykiss) produced by fused sperm

1995 ◽  
Vol 52 (5) ◽  
pp. 892-896 ◽  
Author(s):  
Kazuo Araki ◽  
Hisako Shinma ◽  
Hiroyuki Nagoya ◽  
Ichiro Nakayama ◽  
Hiroshi Onozato
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Seminal Plasma ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Wild Type ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Cryopreserved Sperm ◽  
Γ Ray

Androgenesis is a technique that produces individuals with all of their chromosomes from male parents. It can be useful in generating homozygous lines of fish, and in the recovery of species from cryopreserved sperm. We produced androgenetic diploids by using fused sperm to fertilize eggs. Eggs of albino "Yellow" rainbow trout (Oncorhynchus mykiss) were exposed to 60Co γ-ray irradiation to inactivate the maternally-derived nuclear DNA, and were then fertilized with wild-type rainbow trout sperm fused using an artificial seminal plasma containing 85 mM CaCl2. Embryos that reached the hatching stage (average 0.11%) were pigmented. The nuclear DNA content of cells from newly hatched fry was the same as the DNA content of erythrocyte nuclei of normal diploids.

Protective role of ascorbic acid against damage to male germ cells in rainbow trout (Oncorhynchus mykiss)

1999 ◽  
Vol 56 (2) ◽  
pp. 178-183 ◽  
Author(s):  
Andrzej Ciereszko ◽  
Konrad Dabrowski ◽  
Feng Lin ◽  
Li Liu
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Vitamin C ◽  
Oncorhynchus Mykiss ◽  
Seminal Plasma ◽  
Germ Cells ◽  
Plasma Ascorbic Acid ◽  
Male Germ Cells ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Low Levels

Low antioxidant levels have been implicated in damage to sperm DNA. We used a teleost fish to test if low paternal vitamin C status may cause mutations to the sperm and birth defects. During spermatogenesis, rainbow trout (Oncorhynchus mykiss) males were fed diets with graded levels of ascorbic acid derivative that were below and severalfold higher than those required for maximum growth. This treatment resulted in corresponding changes in levels of vitamin C in seminal plasma. We found that low levels of vitamin C in seminal plasma of rainbow trout were associated with a high percentage of abnormal embryos in the offspring. Among abnormal embryos, 34.8% were haploids or aneuploids, as revealed by flow cytometric measurement of DNA content. We found that UV-irradiated sperm gave rise to progeny with abnormalities similar to those resulting from sperm with low levels of antioxidants in seminal plasma. Ascorbic acid applied directly to semen did not prevent damage due to UV irradiation. These results provide the first experimental evidence that the lack or low levels of ascorbic acid in semen correlate with damage to male germ cells.

scholarly journals Oxidative stress and antioxidant enzyme defence system in seminal plasma of common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss) during spawning season

2018 ◽  
Vol 63 (No. 2) ◽  
pp. 78-84 ◽  
Author(s):  
A. Shaliutina-Kolešová ◽  
N. Rui ◽  
S. Ashtiani ◽  
M. Rodina ◽  
J. Cosson ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Antioxidant Enzyme ◽  
Seminal Plasma ◽  
Cyprinus Carpio ◽  
Spawning Season ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Assessment of seminal oxidative stress indicators is emerging as a valuable prognostic tool in assisted reproductive technology. We investigated levels of oxidative stress caused by reactive oxygen species (ROS) and the antioxidant enzyme defence system comprising superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx) in seminal plasma (SP) of common carp Cyprinus carpio and rainbow trout Oncorhynchus mykiss over the course of the spawning season. Oxidation was determined in lipids and proteins by assessing thiobarbituric acid reactive species (TBARS) and the 2,4-dinitrophenylhydrazine carbonyl groups, respectively. Levels of SOD were assessed by the autoxidation of pyrogallol. Determination of GR and GPx was based on the rate of NADPH oxidation. We observed clear alterations in lipids and proteins over the course of the spawning season in both species. The highest levels of TBARS and CP were recorded late in the season. SOD was not significantly altered in either species. The activity of GR was higher in carp SP late in spawning compared to other times (P < 0.05), while in rainbow trout, GR significantly increased (24.13 ± 2.8 mU/mg protein) in mid-season. A significantly lower GPx activity (9.18 ± 1.32 mU/mg protein) was found in rainbow trout SP early in the spawning season, but no significant differences in GPx were observed over the course of the season in carp. These results provide further understanding of the role of fish SP antioxidants and present new data on the oxidant and antioxidant balance in SP during the spawning season that may be of value in the development of methods for artificial reproduction of teleost species.

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