Protective effect of dry olive leaf extract in adrenaline induced DNA damage evaluated using in vitro comet assay with human peripheral leukocytes

Almost 30% of US residents ages 65 and older have diabetes. The cost of diabetes care was estimated at $174 billion in 2007, including $116 billion in additional medical costs, and $58 billion in reduced productivity. Globally, the estimated cost of diabetes care was $376 billion in 2010, representing 12% of health expenditures. Many individuals with diabetes make use of functional foods, nutritional supplements, and/or herbal remedies to manage their disease. The functional foods olive leaf extract, turmeric, and fenugreek are commonly used in traditional medicine systems to manage diabetes. All three of these functional foods have antioxidant and anti-inflammatory properties as well as specific insulin sensitizing qualities. In vitro studies offer proof of mechanism, and animal studies consistently show treatment efficacy for all three foods. The few human studies that have been conducted, however, use surrogate rather than clinical endpoints. The establishment of these and other functional foods as evidence based interventions for diabetes requires well designed, adequately powered, and randomized controlled pivotal trials with clinical endpoints.Keywords: type 2 diabetes, olive leaf extract, turmeric, and fenugreek

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Study on the protective effect of hydroalcoholic Olive Leaf extract (oleuropein) on the testis and sperm parameters in adult male NMRI mice exposed to Mancozeb

Gene Reports ◽

10.1016/j.genrep.2020.100870 ◽

2020 ◽

Vol 21 ◽

pp. 100870

Author(s):

Maryam Ashkanani ◽

Bahar Farhadi ◽

Erfan Ghanbarzadeh ◽

Hakimeh Akbari

Keyword(s):

Protective Effect ◽

Adult Male ◽

Leaf Extract ◽

Sperm Parameters ◽

Olive Leaf ◽

Nmri Mice ◽

Olive Leaf Extract

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Evolution of the phenolic compounds profile of olive leaf extract encapsulated by spray-drying during in vitro gastrointestinal digestion

Food Chemistry ◽

10.1016/j.foodchem.2018.11.127 ◽

2019 ◽

Vol 279 ◽

pp. 40-48 ◽

Cited By ~ 22

Author(s):

Estefanía González ◽

Ana María Gómez-Caravaca ◽

Begoña Giménez ◽

Rubén Cebrián ◽

Mercedes Maqueda ◽

...

Keyword(s):

Phenolic Compounds ◽

Spray Drying ◽

Leaf Extract ◽

Olive Leaf ◽

Gastrointestinal Digestion ◽

Olive Leaf Extract ◽

In Vitro Gastrointestinal Digestion

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Indirect Chronic Effects of an Oleuropein-Rich Olive Leaf Extract on Sucrase-Isomaltase In Vitro and In Vivo

Nutrients ◽

10.3390/nu11071505 ◽

2019 ◽

Vol 11 (7) ◽

pp. 1505

Author(s):

Alison Pyner ◽

Shuk Yan Chan ◽

Sarka Tumova ◽

Asimina Kerimi ◽

Gary Williamson

Keyword(s):

Leaf Extract ◽

Cell Model ◽

Area Under The Curve ◽

Surface Protein ◽

Intestinal Cell ◽

Olive Leaf ◽

Sucrose Hydrolysis ◽

Olive Leaf Extract

Consumption of dietary bioactives is an avenue to enhancing the effective healthiness of diets by attenuating the glycaemic response. The intestinal brush border enzyme sucrase-isomaltase (SI) is the sole enzyme hydrolysing consumed sucrose, and we previously showed the acute effects of olive leaf extract (OLE) on sucrase activity when given together with sugars both in vitro and in vivo. Here we tested whether OLE could affect sucrase expression when pre-incubated chronically, a “priming” effect not dependent on competitive interaction with SI, in both a cell model and a human intervention. Using differentiated Caco-2/TC7 cells, long-term pre-treatment with oleuropein-rich olive leaf extract (OLE) lowered SI mRNA, surface protein and activity, and attenuated subsequent sucrose hydrolysis. Based on these results, a randomised, double-blinded, placebo-controlled, crossover pilot study was conducted. OLE (50 mg oleuropein) was consumed in capsule form 3 times a day for 1 week by 11 healthy young women followed by an oral sucrose tolerance test in the absence of OLE. However this treatment, compared to placebo, did not induce a change in post-prandial blood glucose maximum concentration (Glcmax), time to reach Glcmax and incremental area under the curve. These results indicate that changes in SI mRNA, protein and activity in an intestinal cell model by OLE are not sufficient under these conditions to induce a functional effect in vivo in healthy volunteers.

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