Corrigendum to ‘Inflammatory and tumorigenic effects of environmental pollutants found in particulate matter on lung epithelial cells’ [Toxicol In Vitro 59 (2019) 300–311]

Particulate matter (PM) exposure induces a pathological response from both the lungs and the cardiovascular system. PM is capable of both manifestation into the lung epithelium and entrance into the bloodstream. Therefore, PM has the capacity for both direct and lung-mediated indirect effects on the heart. In the present studies, we exposed isolated rat cardiomyocytes to ultrafine particulate matter (diesel exhaust particles, DEP) and examined their contractile function and calcium handling ability. In another set of experiments, lung epithelial cells (16HBE14o- or Calu-3) were cultured on permeable supports that allowed access to both the basal (serosal) and apical (mucosal) media; the basal media was used to culture cardiomyocytes to model the indirect, lung-mediated effects of PM on the heart. Both the direct and indirect treatments caused a reduction in contractility as evidenced by reduced percent sarcomere shortening and reduced calcium handling ability measured in field-stimulated cardiomyocytes. Treatment of cardiomyocytes with various anti-oxidants before culture with DEP was able to partially prevent the contractile dysfunction. The basal media from lung epithelial cells treated with PM contained several inflammatory cytokines, and we found that monocyte chemotactic protein-1 was a key trigger for cardiomyocyte dysfunction. These results indicate the presence of both direct and indirect effects of PM on cardiomyocyte function in vitro. Future work will focus on elucidating the mechanisms involved in these separate pathways using in vivo models of air pollution exposure.

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Inflammatory and tumorigenic effects of environmental pollutants found in particulate matter on lung epithelial cells

Toxicology in Vitro ◽

10.1016/j.tiv.2019.05.022 ◽

2019 ◽

Vol 59 ◽

pp. 300-311 ◽

Cited By ~ 1

Author(s):

İsa Taş ◽

Rui Zhou ◽

So-Yeon Park ◽

Yi Yang ◽

Chathurika D.B. Gamage ◽

...

Keyword(s):

Particulate Matter ◽

Epithelial Cells ◽

Environmental Pollutants ◽

Lung Epithelial Cells ◽

Lung Epithelial

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Difference on oxidative stress in lung epithelial cells and macrophages induced by ambient fine particulate matter (PM2.5)

Air Quality Atmosphere & Health ◽

10.1007/s11869-020-00835-5 ◽

2020 ◽

Vol 13 (7) ◽

pp. 789-796 ◽

Cited By ~ 1

Author(s):

Ran Li ◽

Yixuan Wang ◽

Xinghua Qiu ◽

Fanfan Xu ◽

Rucheng Chen ◽

...

Keyword(s):

Oxidative Stress ◽

Particulate Matter ◽

Epithelial Cells ◽

Fine Particulate Matter ◽

Lung Epithelial Cells ◽

Fine Particulate ◽

Lung Epithelial

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Effect of Slit/Robo signaling on regeneration in lung emphysema

Experimental & Molecular Medicine ◽

10.1038/s12276-021-00633-8 ◽

2021 ◽

Author(s):

Jin-Soo Park ◽

RyeonJin Cho ◽

Eun-Young Kang ◽

Yeon-Mok Oh

Keyword(s):

Epithelial Cells ◽

Mouse Model ◽

Lung Epithelial Cells ◽

Mouse Lung ◽

Chronic Obstructive ◽

Irreversible Damage ◽

Lung Epithelial ◽

And Migration ◽

Proliferation And Migration

AbstractEmphysema, a pathological component of chronic obstructive pulmonary disease, causes irreversible damage to the lung. Previous studies have shown that Slit plays essential roles in cell proliferation, angiogenesis, and organ development. In this study, we evaluated the effect of Slit2 on the proliferation and migration of mouse lung epithelial cells and its role in regeneration in an emphysema lung mouse model. Here, we have shown that Slit2/Robo signaling contributes to the regeneration of lungs damaged by emphysema. Mouse epithelial lung cells treated with Slit2 exhibited increased proliferation and migration in vitro. Our results also showed that Slit2 administration improved alveolar regeneration in the emphysema mouse model in vivo. Furthermore, Slit2/Robo signaling increased the phosphorylation of ERK and Akt, which was mediated by Ras activity. These Slit2-mediated cellular signaling processes may be involved in the proliferation and migration of mouse lung epithelial cells and are also associated with the potential mechanism of lung regeneration. Our findings suggest that Slit2 administration may be beneficial for alveolar regeneration in lungs damaged by emphysema.

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