216. Experimental, Immunochemical Reactivity and Neutralizing Capacity of Rhinocerophis (Bothrops) alternatus Antivenoms, from Distinct Geographical Sources

Toxicon ◽  
2012 ◽  
Vol 60 (2) ◽  
pp. 206
Author(s):  
L.C. Lanari ◽  
A. Olvera ◽  
V. Costa de Oliveira ◽  
R.D. Laskowicz ◽  
P.I. Regner ◽  
...  
2016 ◽  
Vol 5 (04) ◽  
pp. 4524
Author(s):  
Abdullah Shaikh Farooque ◽  
Md. Azharuddin Ismail Atar*

Medicinal plants are being widely used, either as single drug or in combination in health care delivery system. Indian Sarsaparilla, Hemidesmus indicus (Family: Asclepiadaceae) is a commonly known Indian Medicinal Plant, which is widely recognized in traditional systems of Medicine. It contains various phytoconstituents belonging to the category glycosides, flavonoids, tannins, sterols and volatile oils. It has been reported as useful in biliousness, blood diseases, dysentery, diarrhea, respiratory disorders, skin diseases, syphilis, fever, leprosy, leucoderma, leucorrhoea, itching, bronchitis, asthma, eye diseases, epileptic fits in children, kidney and urinary disorders, loss of appetite, burning sensation, dyspepsia, nutritional disorders, ulcer and rheumatism. Several studies are being carried towards its activities like analgesic, anti-inflammatory, antiulcer, hepatoprotective, antioxidant and helicobactericidal properties. In our study we have evaluated antacid activity of sariva (Anantmool) by using In-Vitro method, i.e. ANC (Acid Neutralizing Capacity). This evaluation was done by comparing the ANC of sariva macerated & powdered drug with water as blank & standard drug i.e. NaHCO3. Based on this In-Vitro experiment, we can conclude that, the macerated & powdered drug of sariva (Anantmool) evaluated in this study, varied in potency as measured in terms of their ANC. These results having ** i.e. P < 0.01 & Passed the normality test. However, the present study being in-vitro, the effects of antacid may vary In-Vitro; individual variations also contribute to the ultimate effectiveness of as antacid.        


Pathogens ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 192 ◽  
Author(s):  
Sanae Akkaoui ◽  
Anders Johansson ◽  
Maâmar Yagoubi ◽  
Dorte Haubek ◽  
Adnane El hamidi ◽  
...  

In this study, the essential oil of Origanum vulgare was evaluated for putative antibacterial activity against six clinical strains and five reference strains of Aggregatibacter actinomycetemcomitans, in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography–mass spectrometry coupled (CG–MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), p-cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the A. actinomycetemcomitans strains. O. vulgare essential oil did not interfere with the neutralizing capacity of Psidium guajava against the A. actinomycetemcomitans leukotoxin. In addition, it was shown that the O. vulgare EO had an antibacterial effect against A. actinomycetemcomitans on a similar level as some tested antimicrobials. In view of these findings, we suggest that O.vulgare EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to A. actinomycetemcomitans. In addition, it can be used together with the previously tested leukotoxin neutralizing Psidium guajava.


Virology ◽  
1995 ◽  
Vol 211 (1) ◽  
pp. 339-344 ◽  
Author(s):  
Tahar Babas ◽  
Besma Belhadj-Jrad ◽  
Roger Le Grand ◽  
Dominique Dormont ◽  
Luc Montagnier ◽  
...  

2021 ◽  
Vol 184 ◽  
pp. 56-59
Author(s):  
Igor R. Santos ◽  
Anderson L. Carvalho ◽  
Luan C. Henker ◽  
Marcele B. Bandinelli ◽  
Lívia E. Surita ◽  
...  

Vaccines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 742
Author(s):  
José Javier Morales-Núñez ◽  
José Francisco Muñoz-Valle ◽  
Carlos Meza-López ◽  
Lin-Fa Wang ◽  
Andrea Carolina Machado Sulbarán ◽  
...  

The main expected result of a vaccine against viruses is the ability to produce neutralizing antibodies. Currently, several vaccines against SARS-CoV-2 are being applied to prevent mortal complications, being Pfizer-BioNTech (BNT162b2) one of the first to be authorized in the USA and Mexico (11 December 2020). This study evaluated the efficacy of this vaccine on antibody production with neutralizing capacity and its side effects in healthcare workers with and without prior SARS-CoV-2 infection and in a group of unvaccinated individuals with prior COVID-19. The main findings are the production of 100% neutralizing antibodies in both groups after the second dose, well-tolerated adverse effects, the possible presence of immunosenescence, and finally, we support that a single dose of this vaccine in individuals with prior COVID-19 would be sufficient to achieve an immunization comparable to people without prior COVID-19 with a complete vaccination program (2 doses).


Toxicon ◽  
2014 ◽  
Vol 86 ◽  
pp. 89-95 ◽  
Author(s):  
María E. Garcia Denegri ◽  
Silvana Maruñak ◽  
Juan S. Todaro ◽  
Luis A. Ponce-Soto ◽  
Ofelia Acosta ◽  
...  

1984 ◽  
Vol 26 (5) ◽  
pp. 247-253 ◽  
Author(s):  
L. S. Queiroz ◽  
C. A. Petta
Keyword(s):  

Veneno bruto de urutu (Bothrops alternatus) dissolvido em solução salina fisiológica foi injetado no músculo tibial anterior direito de camundongos adultos na dose de 80 μg. Os músculos foram examinados em cortes de parafina, corados por Hematoxilina e Eosina. Aos 10 minutos já havia intensa hemorragia difusa no M. tibial anterior, mas apenas raras fibras musculares estavam necróticas. Nas horas seguintes, contudo, observou-se rápido aumento do número de fibras afetadas, sendo que às 24 hs o músculo apresentava-se totalmente necrótico. Vasos sangüíneos intramusculares e nas proximidades do M. tibial anterior mostravam necrose hialina da camada média e por vezes trombose. A fagocitose dos restos celulares ocorreu da periferia para o centro e acompanhou-se de regeneração muscular. Após 1 a 2 meses, em vários animais houve recuperação considerável do músculo, embora com persistência de cicatriz. As fibras regeneradas possuiam núcleos centrais e variavam em diâmetro, estando muitas atróficas. Em outros camundongos a regeneração do M. tibial anterior foi muito precária, tendo este sido substituído por tecido fibroadiposo com apenas raras fibras musculares. Os resultados mostram que, apesar da gravidade das lesões iniciais devidas ao veneno, ocorre regeneração muscular em grau variável de animal para animal. Sugere-se que a má regeneração observada em alguns casos poderia ser devida, ao menos em parte, a dano vascular permanente.


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