PHARMACOGNOSTICAL STUDIES OF LEAVES OF ACACIA ETBAICA SUBSPECIES UNCINATA

bjective: The objective of the study is to analyze the microscopic, macroscopic, and physicochemical standards of the leaves of the Acacia etbaica subspecies uncinata. Methods: Pharamacognostic studies (macroscopic, microscopic, and powder microscopy) were carried out. Physicochemical standards (ash values, extractives values, and moisture content by loss on drying) were determined. Fluorescence analysis of powdered drug was also performed. Results: The macroscopic study showed that the leaves were bipinnate with 3–11 pairs of pinnae, each containing 7–25 pairs of leaflets. The leaflet was linear with parallel margins and a rounded at the apex, color was dark green, odor was characteristic and the taste was astringent. The characteristic microscopy of leaves showed the presence of polygonal and rectangular epidermal cells in the center of the lamina and rectangular at the edges, paracytic stomata, non-glandular trichomes, and reticulate venations. The microscopic study of petiole, rachis, and rachilla revealed the presence of elongate, swollen conical-shaped, flagelliform, and wavy trichomes. The powder microscopy also revealed paracytic stomata, trichomes with pedestals, and annular vessels. Physiochemical analysis of dried leaf powder showed total ash, acid insoluble ash, water-soluble ash, water extractive value, ethanol extractive value, and moisture content as 6.11%, 2.50%, 4.57%, 32.50%, 14.10%, and 7.26% w/w respectively. The fluorescence analysis of leaf powder was established. Conclusion: Various pharmacognostic, physicochemical, and fluorescence parameters observed in this study will help in the identification and standardization of the leaves of A. etbaica subspecies uncinata.

Pharmacognostical Study, Phytochemical and Physicochemical Evaluation and Establishment of Quality Standards for Certain Traditional Antidiabetic Medicinal Plants

Aim: The present work was aimed at evolving pharmacognostical and phytochemical quality standards for certain traditional herbs like Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum. These three plants are reported to possess antidiabetic activity. Study Design: The plants were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC) Place and Duration of Study: The study was carried out at College of Pharmacy, Dr. APJ Abdul Kalam University, Indore, during 2019-20 Methodology: The material were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC) profile of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum were determined using standard methods. Results: The macroscopical and microscopical studies revealed useful diagnostic features. Phytochemical screening reveals the presence of secondary metabolites, physicochemical including fluorescence analysis of powdered drug proved useful to differentiate the powdered drug material. Thin layer chromatography analysis showed the presence of important phytoconstituents such Phyllanthin, hypophyllanthin, glycerrizin and piperine Conclusion: The data generated from this study would serve as useful gauge for determining the quality of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum thereby correct identification and authentification of these plants. It would also help scientists to utilize such needful information regarding the plants identity and characteristics in building new polyherbal formulations.

Comprehensive Pharmacognostical Profile of Drynaria quercifolia (L.) J. Sm.

Pteridophytes are gaining importance as therapeutic agents due to the presence of various phytochemicals and their promising bioactivities. Drynaria quercifolia (L.) J. Sm., a Polypodiaceae member is endowed with numerous medicinal properties and finds wide usage in ethno as well as traditional medicines. The rhizome of D. quercifolia (L.) J. Sm. was subjected to macro-microscopic, physicochemical, phytochemical and HPTLC analysis to derive a standard for this drug. The microscopic detailing showed a wavy outline due to the presence of ridges and furrows and a broad ground tissue with diffusely arranged steles. The powdered drug showed trichomes, stellar tissue and silica crystals while the physicochemical and phytochemical screenings gave substantial values of different parameters. The rhizome extracts were subjected to HPTLC studies with Linomat 5 TLC applicator and diagnostic peaks were recorded under UV 254 nm, 366 nm and 620 nm. The study put forward an exclusive identity profile of this medicinal rhizome.

Pharmacognostical and Phytochemical Analysis of Ayurveda Medicine (Atibala) Abutilon indicum (Linn.) Sweet Roots Collected From Field

Atibala (Abutilon indicum Linn.) Sweet belonging to Family Malvaceae is very important plant since long in traditional medicine of India and grows in waste places. The drug is attributed Balya Kantivardhaka, Grahi, Ojovardhaka, Brinhana, Krimighna, Dahahara, Vatahara, Rasayana, Mutrajanana, Mridurechana, Vajikara, Kasahara and Vedanahara properties in different Ayurvedic classical texts. Pharmacognostical and phytochemical studies are essential for proper identification and authentication of the drug. Various methods including microscopy, physiochemical contents and phytochemical estimation along with quantitative analysis were conducted to evaluate Pharmacognostical, physiochemical and preliminary phytochemical standards of Atibala (Abutilon indicum (Linn.) Sweet roots. These studies can be helpful to define diagnostic features for identification and standardization of both intact and powdered drug. The organoleptic examination of the intact and powdered materials of roots of Atibala revealed light brown color, odourless odour and astringent and bitter taste. After analysing result of present study, we found presence of carbohydrates, tannins, protein, alkaloid, saponin, amino acids and phenolic compounds in both alcoholic and aqueous extract. Present study will be helpful in ensuring efficacy of the drug, safety, purity and about adulteration of drug.

Macro and microscopic evaluation of Gmelina arborea Roxb. – A botanical pharmacognostic approach for quality control of raw drug material

Gmelina arborea Roxb. of family Verbenaceae, is one of the highly valued medicinal plant used in numerous traditional medical formulations. It belongs to the ‘Dasamoola’ group of ayurvedic medicinal plants and hence widely exploited. Pharmacognostic techniques involving macroscopic, microscopic and also dry powder analysis serve as botanical methods which help in the correct identification of the crude drug. Leaf constants such as stomatal number, stomatal index, vein islet number, vein termination number also have been determined. Better quality control practices in nutraceutical and pharmaceutical industries demand the correct identification of the dried plants or powdered drug thereby detects and prevents the adulterations, if any. The challenge ahead of this investigation is to validate the therapeutic efficacy and safety of the plant following standard methodology.

Evaluation of Hemidesmus indicus root as an antacid by In vitro

Medicinal plants are being widely used, either as single drug or in combination in health care delivery system. Indian Sarsaparilla, Hemidesmus indicus (Family: Asclepiadaceae) is a commonly known Indian Medicinal Plant, which is widely recognized in traditional systems of Medicine. It contains various phytoconstituents belonging to the category glycosides, flavonoids, tannins, sterols and volatile oils. It has been reported as useful in biliousness, blood diseases, dysentery, diarrhea, respiratory disorders, skin diseases, syphilis, fever, leprosy, leucoderma, leucorrhoea, itching, bronchitis, asthma, eye diseases, epileptic fits in children, kidney and urinary disorders, loss of appetite, burning sensation, dyspepsia, nutritional disorders, ulcer and rheumatism. Several studies are being carried towards its activities like analgesic, anti-inflammatory, antiulcer, hepatoprotective, antioxidant and helicobactericidal properties. In our study we have evaluated antacid activity of sariva (Anantmool) by using In-Vitro method, i.e. ANC (Acid Neutralizing Capacity). This evaluation was done by comparing the ANC of sariva macerated & powdered drug with water as blank & standard drug i.e. NaHCO3. Based on this In-Vitro experiment, we can conclude that, the macerated & powdered drug of sariva (Anantmool) evaluated in this study, varied in potency as measured in terms of their ANC. These results having ** i.e. P < 0.01 & Passed the normality test. However, the present study being in-vitro, the effects of antacid may vary In-Vitro; individual variations also contribute to the ultimate effectiveness of as antacid.

Innovations in the training of the respiratory muscles (literature review)

A developed stand of physical modeling of inhalation allows us to estimate the distribution of the powdered drug in the respiratory tract, depending upon the device and method of inhalation. The created means of mathematical and physical modeling of inhalation can be used in the purchase of dosage forms overseas pro-duction to determine the most effective models. The authors propose and justify a new way of training of the respiratory muscles and the corresponding device implementing peak load in the initial phase of inhalation and exhalation. Most effectiveness of the proposed respiratory simulator compared to simulators throttle type is identified.

Pharmacognostical and Preliminary Phytochemical Screening of the Leaf Extract of Jasminum auriculatum Vahl

Jasminum auriculatum belonging to the family Oleaceae are reported to have good medicinal values in traditional system of medicines. The present study deals with pharmacognostical examination of morphological and microscopical characters and phytochemical investigations of Jasminumauriculatum leaves including determination of loss on drying, ash values and extractive values. The preliminary phytochemical screening of powdered drug was also carried out, the qualitative chemical examinations revealed the presence of various phytoconstituents like alkaloids, steroids, terpenoids, saponins, carbohydrates, Glycosides, protein, mucilages, phenols ,tannins and flavonoids.

Dry powder inhalers and the right things to remember: a concept review

Dry powder inhalers (DPIs) are widely and increasingly used in clinical practice because they represent a substantial advancement in inhalation technology. The effectiveness of a powdered drug to inhale depends on the inspiratory flow rate generated by the patient and on the turbulence produced by the intrinsic resistance of the DPI. While the inspiratory flow is variable with the patient’s ability and conditions, the turbulence is differently sized within each device because depending of its technical design. There are higher - medium-, and low-resistance devices. With low-resistance DPIs, the disaggregation and the microdispersion of the drug highly depend on the patient’s inhalation airflow rate, because the role of the resistance-induced turbulence is obviously negligible in these cases. This flow-rate dependency is minimized in the presence of a sufficient regimen of turbulence as in the case of medium-resistance DPIs. Both the disaggregation and the micro-dispersion of the powdered drug are optimized in these circumstances even in the absence of a maximal inspiratory flow rate. The low resistance DPIs should not be regarded as the best performer DPIs because their intrinsic low-resistance regimen requires a higher inspiratory airflow rate and effort, which frequently cannot be achieved by subjects suffering from a disease-induced airflow limitation. Only when the ratio between the inhalation flow rate and the DPI intrinsic resistance is balanced, the speed of the particulate, the distribution of the drug within the lung, and the variability of the effective inhaled dose are optimized.